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Clinical Chemistry and Laboratory Medicine (CCLM)

Published in Association with the European Federation of Clinical Chemistry and Laboratory Medicine (EFLM)

Editor-in-Chief: Plebani, Mario

Ed. by Gillery, Philippe / Lackner, Karl J. / Lippi, Giuseppe / Melichar, Bohuslav / Payne, Deborah A. / Schlattmann, Peter / Tate, Jillian R.

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Volume 48, Issue 6 (Jun 2010)


New quantitative electrochemiluminescence method (ECLIA) for interleukin-6 (IL-6) measurement

Belén Prieto / Diego Miguel / Marta Costa / Daniel Coto / Francisco V. Álvarez
  • Clinical Biochemistry Laboratory, Hospital Universitario Central de Asturias, Oviedo, Spain
  • Department of Biochemistry and Molecular Biology, Universidad de Oviedo, Oviedo, Asturias, Spain
  • Other articles by this author:
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Published Online: 2010-03-19 | DOI: https://doi.org/10.1515/CCLM.2010.153


Background: A new interleukin-6 (IL-6) electrochemiluminescent immunoassay (ECLIA, Roche Diagnostics) was evaluated and compared to a previous semiquantitative immunoassay (Milenia Biotec). Reference ranges for cord blood plasma were also calculated.

Methods: The new IL-6 ECLIA test was performed using a Cobas E601 analyzer (Roche Diagnostics). The comparison method (Milenia Biotec) was a semiquantitative lateral flow immunoassay, coupled to a digital image capture system (PicoScan). Healthy at term newborns were recruited to establish reference ranges for IL-6 in cord blood plasma.

Results: Total imprecision ranged from 3.7% to 8.0% depending on the IL-6 concentrations. The calculated limit of detection for IL-6 measured by ECLIA was 2.63 pg/mL, almost twice as high as that claimed by the manufacturer (1.5 pg/mL). The linearity of the method was verified to 5000 pg/mL. The IL-6 reference limit, obtained from 148 cord blood samples, was 30 pg/mL (90% confidence interval: 19–48 pg/mL).

Conclusions: The new quantitative ECLIA method showed good reproducibility, linearity and functional sensitivity. Additional clinically based studies are needed to elucidate the usefulness of the IL-6 reference limit, calculated here for the first time by ECLIA in cord blood plasma, to aid in the diagnosis of vertical transmission of sepsis.

Clin Chem Lab Med 2010;48:835–8.

Keywords: cord blood IL-6; interleukin-6 (IL-6); neonatal sepsis

About the article

Corresponding author: Francisco V. Álvarez, PhD, Servicio de Bioquímica Clínica, Hospital Universitario Central de Asturias, 33006 Oviedo, Spain Phone/Fax: +34 985108073,

Received: 2009-11-11

Accepted: 2010-01-17

Published Online: 2010-03-19

Published in Print: 2010-06-01

Citation Information: Clinical Chemistry and Laboratory Medicine, ISSN (Online) 1437-4331, ISSN (Print) 1434-6621, DOI: https://doi.org/10.1515/CCLM.2010.153.

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©2010 by Walter de Gruyter Berlin New York. Copyright Clearance Center

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