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Clinical Chemistry and Laboratory Medicine (CCLM)

Published in Association with the European Federation of Clinical Chemistry and Laboratory Medicine (EFLM)

Editor-in-Chief: Plebani, Mario

Ed. by Gillery, Philippe / Lackner, Karl J. / Lippi, Giuseppe / Melichar, Bohuslav / Payne, Deborah A. / Schlattmann, Peter / Tate, Jillian R.

12 Issues per year


IMPACT FACTOR 2017: 3.556

CiteScore 2017: 2.34

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Source Normalized Impact per Paper (SNIP) 2017: 1.188

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1437-4331
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Volume 49, Issue 11

Issues

Phenotype of Gc-globulin influences the macrophage activating factor (MAF) levels in serum

Evi Debruyne
  • Department of Clinical Chemistry, Microbiology and Immunology, Ghent University Hospital, Ghent, Belgium
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Marijn Speeckaert
  • Department of Clinical Chemistry, Microbiology and Immunology, Ghent University Hospital, Ghent, Belgium
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Yannick Vande Weygaerde
  • Department of Clinical Chemistry, Microbiology and Immunology, Ghent University Hospital, Ghent, Belgium
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Joris Delanghe
  • Department of Clinical Chemistry, Microbiology and Immunology, Ghent University Hospital, Ghent, Belgium
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
Published Online: 2011-08-23 | DOI: https://doi.org/10.1515/cclm.2011.676

Abstract

Background: Gc-globulin is a polymorphic protein with three phenotypes: Gc 1-1, Gc 2-1 and Gc 2-2. Deglycosylation of Gc-globulin results in a Gc-macrophage activating factor (Gc-MAF). This study investigated the potential of MAF as a tumour marker and the influence of Gcphenotypes on serum MAF-concentrations.

Methods: Gc-phenotype of 98 healthy individuals and 60 cancer patients was determined. MAF-levels of healthy individuals and cancer patients were analysed according to their Gc-phenotype using a Helix pomatia agglutinin-based ELISA. ROC curves analysed the efficiency of MAF as a tumour marker.

Results: MAF-levels between controls and patients were significantly different (p<0.001). No phenotypic differences were found in the patients. In comparison with the controls, MAF-values were significantly lower in cancer patients carrying Gc 1-1 (p<0.01) and Gc 2-1 (p<0.001). No difference was observed in Gc 2-2 phenotype. Diagnostic accuracy of MAF as a tumour marker also demonstrated pronounced differences between Gc-phenotypes.

Conclusions: Gc-phenotype is a confounding factor when interpreting MAF-data. The value of MAF as a tumour marker varies according to phenotype. Future studies on MAF will have to consider the effect of Gc-phenotype.

Keywords: Gc-globulin; Gc-MAF; Helix pomatia agglutinin; lectin-based ELISA; phenotype; tumour marker

About the article

Corresponding author: Joris Delanghe, Department of Clinical Chemistry, Ghent University Hospital, De Pintelaan 185, 9000 Gent, Belgium Phone: +32 9 332 29 56, Fax: +32 9 332 36 59,


Received: 2011-05-23

Accepted: 2011-07-25

Published Online: 2011-08-23

Published in Print: 2011-11-01


Citation Information: Clinical Chemistry and Laboratory Medicine, Volume 49, Issue 11, Pages 1855–1860, ISSN (Online) 1437-4331, ISSN (Print) 1434-6621, DOI: https://doi.org/10.1515/cclm.2011.676.

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