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Clinical Chemistry and Laboratory Medicine (CCLM)

Published in Association with the European Federation of Clinical Chemistry and Laboratory Medicine (EFLM)

Editor-in-Chief: Plebani, Mario

Ed. by Gillery, Philippe / Lackner, Karl J. / Lippi, Giuseppe / Melichar, Bohuslav / Payne, Deborah A. / Schlattmann, Peter


IMPACT FACTOR 2017: 3.556

CiteScore 2017: 2.34

SCImago Journal Rank (SJR) 2017: 1.114
Source Normalized Impact per Paper (SNIP) 2017: 1.188

Online
ISSN
1437-4331
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Volume 49, Issue 12

Issues

Rapid and simple IgG specific test for the exclusion of heparin induced thrombocytopenia (HIT)

Hans-Jürgen Kolde / Ralf Dostatni / Susanne Mauracher
Published Online: 2011-08-29 | DOI: https://doi.org/10.1515/CCLM.2011.693

Abstract

Background: The exclusion of heparin induced thrombocytopenia (HIT) is required for selecting the most appropriate anticoagulation therapy in affected patients. It requires the combination of clinical data with the detection of antibodies directed against platelet factor 4 (PF4) in complex with polyanions (PA) such as heparin.

Methods: We developed a lateral flow immunoassay (LFIA) for PF4/PA complex specific IgG antibodies based on gold nanoparticles. Unlike most other assays, the initial immune reaction takes place in the liquid phase. The sensitivity of the assay has been adjusted with clinical samples aiming in the reliable detection of sera which are positive in a functional platelet activation assay.

Results: Sera from 60 patients with suspected HIT were investigated. LFIA identified correctly all samples (n=20) which were positive in a functional assay (HIPA) and an IgG specific ELISA. It correlated with ELISA, but false positive results were less frequent (7 samples were negative with LFIA and HIPA but positive with ELISA).

Conclusions: The LFIA may be a suitable tool for the rapid exclusion of HIT within 10 min.

Keywords: anticoagulants; heparin induced thrombocytopenia (HIT); immuno assay; sensitivity; specificity

About the article

Corresponding author: Hans-Jürgen Kolde, PhD, Consulting Diagnostics, Gartenstr. 4, 85521 Ottobrunn, Germany Phone: +49 89 6098292, Fax: +49 89 6097580


Received: 2011-07-27

Accepted: 2011-08-01

Published Online: 2011-08-29

Published in Print: 2011-12-01


Citation Information: Clinical Chemistry and Laboratory Medicine (CCLM), Volume 49, Issue 12, Pages 2065–2068, ISSN (Online) 1437-4331, ISSN (Print) 1434-6621, DOI: https://doi.org/10.1515/CCLM.2011.693.

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