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Clinical Chemistry and Laboratory Medicine (CCLM)

Published in Association with the European Federation of Clinical Chemistry and Laboratory Medicine (EFLM)

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Ed. by Gillery, Philippe / Lackner, Karl J. / Lippi, Giuseppe / Melichar, Bohuslav / Payne, Deborah A. / Schlattmann, Peter / Tate, Jillian R.

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Sensitivity and specificity of the high fluorescent lymphocyte count-gate on the Sysmex XE-5000 hematology analyzer for detection of peripheral plasma cells

1 / Gert Jan Vrielink1 / Nicole Tjon-a-Tsoi1 / Henriët Hendriks1 / Wim de Kieviet1 / Edwin ten Boekel1

1Clinical Laboratory, Sint Lucas Andreas Hospital, Amsterdam, The Netherlands

Corresponding author: Dr. E. van Mirre, PhD, Clinical Laboratory, Sint Lucas Andreas Hospital, Jan Tooropstraat 164, 1061 Amsterdam, The Netherlands

Citation Information: Clinical Chemistry and Laboratory Medicine. Volume 49, Issue 4, Pages 685–688, ISSN (Online) 1437-4331, ISSN (Print) 1434-6621, DOI: https://doi.org/10.1515/CCLM.2011.100, March 2011

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Background: Plasma cells are one of the end products of the B-lymphocyte mediated immune response. These cells normally reside in the bone marrow or some peripheral lymphoid tissues. Increased numbers of plasma cells in the blood, usually indicates pathology such as infection, auto-immunity or haematological malignancy. Therefore, the ability to measure plasma cells (PCs) on an automated cell analyzer might be advantageous.

Methods: The performance of the Sysmex XE-5000 leukocyte differential channel [high fluorescent lymphocyte count (HFLC) area] was evaluated for the ability to detect plasma cells in peripheral blood and compared to the detection of plasma cells by flow cytometric analyses.

Results: Our results show that the HFL count from the XE-5000 correlates (R2=0.8) with the number of PCs in peripheral blood, but detects PCs with moderate to good sensitivity (88.9%) and specificity (87.8%).

Conclusions: The Sysmex XE-5000 is suitable for screening blood samples for the presence of elevated number of plasma cells in peripheral blood, but the actual quantification needs to be confirmed by flow cytometry.

Keywords: flow cytometry; hematology analyzer; high fluorescent lymphocyte count (HFLC); plasma cells

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