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Clinical Chemistry and Laboratory Medicine (CCLM)

Published in Association with the European Federation of Clinical Chemistry and Laboratory Medicine (EFLM)

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Ed. by Gillery, Philippe / Lackner, Karl J. / Lippi, Giuseppe / Melichar, Bohuslav / Payne, Deborah A. / Schlattmann, Peter / Tate, Jillian R.

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Identification of Bcl-2/IgH fusion sequences using real-time PCR and chip-based microcapillary electrophoresis

1, a / Yukiko Kawase2, a / Kazunori Miyake1 / Naotake Satoh3 / Nanae Aritaka4 / Yasushi Isobe4 / Kazuo Oshimi4 / Norio Komatsu4 / Takashi Miida1 / Akimichi Ohsaka2

1Department of Clinical Laboratory Medicine, Juntendo University School of Medicine, Tokyo, Japan

2Division of Clinical Laboratory, Juntendo University Hospital, Tokyo, Japan

3Division of Clinical Laboratory, Juntendo Tokyo Koto Geriatric Medical Center, Tokyo, Japan

4Department of Hematology, Juntendo University School of Medicine, Tokyo, Japan

aYoko Tabe and Yukiko Kawase contributed equally to this work.

Corresponding author: Yoko Tabe, MD, PhD, Department of Clinical Laboratory Medicine, Juntendo University School of Medicine, Hongo 2-1-1, Bunkyo-ku, Tokyo 113-8421, Japan Phone: +81-3-3813-3111 (ex) 5187, Fax: +81-3-5804-8637

Citation Information: Clinical Chemistry and Laboratory Medicine. Volume 49, Issue 5, Pages 809–815, ISSN (Online) 1437-4331, ISSN (Print) 1434-6621, DOI: 10.1515/CCLM.2011.141, February 2011

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Background: The determination of polymerase chain reaction (PCR) amplification product sizes of the Bcl-2/IgH fusion gene from follicular lymphoma (FL) provides evidence of clonal identity.

Methods: The present study describes detection of Bcl-2/IgH fusion gene clonality utilizing a small, simple microcapillary electrophoretic chip combined with a real-time PCR method.

Results: The microcapillary electrophoretic chip system effectively detects size differences among the Bcl-2/IgH fusion gene amplification products of FL from patient samples; something that is not possible using traditional gel electrophoresis. We also describe the potential of this system to utilize formalin-fixed, paraffin-embedded tissue samples sectioned on charged slides.

Conclusions: The simple detection of Bcl-2/IgH fusion gene clonality using a microcapillary electrophoretic chip provides reliable information for monitoring minimal residual disease of FL, and can be an effective tool for use in clinical laboratories.

Keywords: Bcl-2/IgH; follicular lymphoma; formalin-fixed paraffin-embedded tissue; microcapillary electrophoretic chip; real-time PCR

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