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Clinical Chemistry and Laboratory Medicine (CCLM)

Published in Association with the European Federation of Clinical Chemistry and Laboratory Medicine (EFLM)

Editor-in-Chief: Plebani, Mario

Ed. by Gillery, Philippe / Lackner, Karl J. / Lippi, Giuseppe / Melichar, Bohuslav / Payne, Deborah A. / Schlattmann, Peter / Tate, Jillian R.

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1437-4331
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Identification of Bcl-2/IgH fusion sequences using real-time PCR and chip-based microcapillary electrophoresis

Yoko Tabe
  • Department of Clinical Laboratory Medicine, Juntendo University School of Medicine, Tokyo, Japan
  • Yoko Tabe and Yukiko Kawase contributed equally to this work.
  • :
/ Yukiko Kawase
  • Division of Clinical Laboratory, Juntendo University Hospital, Tokyo, Japan
  • Yoko Tabe and Yukiko Kawase contributed equally to this work.
/ Kazunori Miyake
  • Department of Clinical Laboratory Medicine, Juntendo University School of Medicine, Tokyo, Japan
/ Naotake Satoh
  • Division of Clinical Laboratory, Juntendo Tokyo Koto Geriatric Medical Center, Tokyo, Japan
/ Nanae Aritaka
  • Department of Hematology, Juntendo University School of Medicine, Tokyo, Japan
/ Yasushi Isobe
  • Department of Hematology, Juntendo University School of Medicine, Tokyo, Japan
/ Kazuo Oshimi
  • Department of Hematology, Juntendo University School of Medicine, Tokyo, Japan
/ Norio Komatsu
  • Department of Hematology, Juntendo University School of Medicine, Tokyo, Japan
/ Takashi Miida
  • Department of Clinical Laboratory Medicine, Juntendo University School of Medicine, Tokyo, Japan
/ Akimichi Ohsaka
  • Division of Clinical Laboratory, Juntendo University Hospital, Tokyo, Japan
Published Online: 2011-02-11 | DOI: https://doi.org/10.1515/CCLM.2011.141

Abstract

Background: The determination of polymerase chain reaction (PCR) amplification product sizes of the Bcl-2/IgH fusion gene from follicular lymphoma (FL) provides evidence of clonal identity.

Methods: The present study describes detection of Bcl-2/IgH fusion gene clonality utilizing a small, simple microcapillary electrophoretic chip combined with a real-time PCR method.

Results: The microcapillary electrophoretic chip system effectively detects size differences among the Bcl-2/IgH fusion gene amplification products of FL from patient samples; something that is not possible using traditional gel electrophoresis. We also describe the potential of this system to utilize formalin-fixed, paraffin-embedded tissue samples sectioned on charged slides.

Conclusions: The simple detection of Bcl-2/IgH fusion gene clonality using a microcapillary electrophoretic chip provides reliable information for monitoring minimal residual disease of FL, and can be an effective tool for use in clinical laboratories.

Keywords: Bcl-2/IgH; follicular lymphoma; formalin-fixed paraffin-embedded tissue; microcapillary electrophoretic chip; real-time PCR

Corresponding author: Yoko Tabe, MD, PhD, Department of Clinical Laboratory Medicine, Juntendo University School of Medicine, Hongo 2-1-1, Bunkyo-ku, Tokyo 113-8421, Japan Phone: +81-3-3813-3111 (ex) 5187, Fax: +81-3-5804-8637


Received: 2010-09-05

Accepted: 2010-12-15

Published Online: 2011-02-11

Published in Print: 2011-05-01


Citation Information: Clinical Chemistry and Laboratory Medicine. Volume 49, Issue 5, Pages 809–815, ISSN (Online) 1437-4331, ISSN (Print) 1434-6621, DOI: https://doi.org/10.1515/CCLM.2011.141, February 2011

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