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Clinical Chemistry and Laboratory Medicine (CCLM)

Published in Association with the European Federation of Clinical Chemistry and Laboratory Medicine (EFLM)

Editor-in-Chief: Plebani, Mario

Ed. by Gillery, Philippe / Greaves, Ronda / Lackner, Karl J. / Lippi, Giuseppe / Melichar, Bohuslav / Payne, Deborah A. / Schlattmann, Peter


IMPACT FACTOR 2017: 3.556

CiteScore 2018: 2.44

SCImago Journal Rank (SJR) 2018: 1.191
Source Normalized Impact per Paper (SNIP) 2018: 1.205

Online
ISSN
1437-4331
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Volume 49, Issue 7

Issues

A simple liquid chromatography-tandem mass spectrometry method for measuring metanephrine and normetanephrine in urine

Jessica Gabler / Abby Miller / Sihe Wang
Published Online: 2011-05-17 | DOI: https://doi.org/10.1515/CCLM.2011.195

Abstract

Background: Measuring urinary fractionated metanephrines is one of the initial tests in the diagnosis of pheochromocytoma. Liquid chromatography-tandem mass spectrometry (LC-MS/MS) represents the most specific and accurate technology for this purpose. The goal of this work was to develop a simple LC-MS/MS method for measuring metanephrines in urine.

Methods: Each urine sample was complexed with diphenylboronic acid, and purified on a Bond-Elute Plexa cartridge. The extract was concentrated and analyzed on a short Atlantis T3 column in 8.5 min. Metanephrines and their deuterated internal standards were monitored in positive electrospray ionization mode by multiple reaction monitoring.

Results: Ion suppression was observed, but was compensated for by the respective internal standard. The analytical measurement range was 0.2–27.4 μmol/L and 0.3–14.6 μmol/L for metanephrine and normetanephrine, respectively. The intra-assay and total coefficient of variation throughout the linear ranges was 2.03%–2.11% and 2.20%–3.80% for metanephrine, and 4.50%–8.09% and 9.00%–10.00% for normetanephrine, respectively. Comparison with a commercial HPLC method using patient samples (n=65) by Passing-Bablok regression showed a slope of 1.000 and 1.014, y-intercept of –0.080 and –0.067, a correlation coefficient of 0.8830 and 0.9022, and a mean difference of 14.0% and –0.43% for metanephrine and normetanephrine, respectively.

Conclusions: This simple method for urine metanephrines is suitable for clinical use.

Keywords: liquid chromatography-tandem mass spectrometry; metanephrines; pheochromocytoma; urine

About the article

Corresponding author: Department of Clinical Pathology, Mail Code L11, 9500 Euclid Ave, Cleveland, OH 44195, USA Phone: +1 216-445-2634, Fax: +1 216-444-4414


Received: 2010-11-13

Accepted: 2011-03-01

Published Online: 2011-05-17

Published in Print: 2011-07-01


Citation Information: Clinical Chemistry and Laboratory Medicine, Volume 49, Issue 7, Pages 1213–1216, ISSN (Online) 1437-4331, ISSN (Print) 1434-6621, DOI: https://doi.org/10.1515/CCLM.2011.195.

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