Jump to ContentJump to Main Navigation
Show Summary Details
More options …

Clinical Chemistry and Laboratory Medicine (CCLM)

Published in Association with the European Federation of Clinical Chemistry and Laboratory Medicine (EFLM)

Editor-in-Chief: Plebani, Mario

Ed. by Gillery, Philippe / Greaves, Ronda / Lackner, Karl J. / Lippi, Giuseppe / Melichar, Bohuslav / Payne, Deborah A. / Schlattmann, Peter

IMPACT FACTOR 2017: 3.556

CiteScore 2018: 2.44

SCImago Journal Rank (SJR) 2018: 1.191
Source Normalized Impact per Paper (SNIP) 2018: 1.205

See all formats and pricing
More options …
Volume 49, Issue 7


A simple liquid chromatography-tandem mass spectrometry method for measuring metanephrine and normetanephrine in urine

Jessica Gabler / Abby Miller / Sihe Wang
Published Online: 2011-05-17 | DOI: https://doi.org/10.1515/CCLM.2011.195


Background: Measuring urinary fractionated metanephrines is one of the initial tests in the diagnosis of pheochromocytoma. Liquid chromatography-tandem mass spectrometry (LC-MS/MS) represents the most specific and accurate technology for this purpose. The goal of this work was to develop a simple LC-MS/MS method for measuring metanephrines in urine.

Methods: Each urine sample was complexed with diphenylboronic acid, and purified on a Bond-Elute Plexa cartridge. The extract was concentrated and analyzed on a short Atlantis T3 column in 8.5 min. Metanephrines and their deuterated internal standards were monitored in positive electrospray ionization mode by multiple reaction monitoring.

Results: Ion suppression was observed, but was compensated for by the respective internal standard. The analytical measurement range was 0.2–27.4 μmol/L and 0.3–14.6 μmol/L for metanephrine and normetanephrine, respectively. The intra-assay and total coefficient of variation throughout the linear ranges was 2.03%–2.11% and 2.20%–3.80% for metanephrine, and 4.50%–8.09% and 9.00%–10.00% for normetanephrine, respectively. Comparison with a commercial HPLC method using patient samples (n=65) by Passing-Bablok regression showed a slope of 1.000 and 1.014, y-intercept of –0.080 and –0.067, a correlation coefficient of 0.8830 and 0.9022, and a mean difference of 14.0% and –0.43% for metanephrine and normetanephrine, respectively.

Conclusions: This simple method for urine metanephrines is suitable for clinical use.

Keywords: liquid chromatography-tandem mass spectrometry; metanephrines; pheochromocytoma; urine

About the article

Corresponding author: Department of Clinical Pathology, Mail Code L11, 9500 Euclid Ave, Cleveland, OH 44195, USA Phone: +1 216-445-2634, Fax: +1 216-444-4414

Received: 2010-11-13

Accepted: 2011-03-01

Published Online: 2011-05-17

Published in Print: 2011-07-01

Citation Information: Clinical Chemistry and Laboratory Medicine, Volume 49, Issue 7, Pages 1213–1216, ISSN (Online) 1437-4331, ISSN (Print) 1434-6621, DOI: https://doi.org/10.1515/CCLM.2011.195.

Export Citation

©2011 by Walter de Gruyter Berlin Boston.Get Permission

Citing Articles

Here you can find all Crossref-listed publications in which this article is cited. If you would like to receive automatic email messages as soon as this article is cited in other publications, simply activate the “Citation Alert” on the top of this page.

Andrew G. Ellis, Philip T. Zeglinski, Kate E. Coleman, and Malcolm J. Whiting
Clinical Mass Spectrometry, 2017
Joanne E Adaway, Brian G Keevil, and Laura J Owen
Annals of Clinical Biochemistry, 2015, Volume 52, Number 1, Page 18
Graeme Eisenhofer, Mirko Peitzsch, and Brett C. McWhinney
TrAC Trends in Analytical Chemistry, 2016, Volume 84, Page 106
Mariëtte Theodora Ackermans and Erik Endert
Bioanalysis, 2014, Volume 6, Number 1, Page 43
Mirko Peitzsch, Daniela Pelzel, Stephan Glöckner, Aleksander Prejbisz, Martin Fassnacht, Felix Beuschlein, Andrzej Januszewicz, Gabriele Siegert, and Graeme Eisenhofer
Clinica Chimica Acta, 2013, Volume 418, Page 50
Joana Bicker, Ana Fortuna, Gilberto Alves, and Amílcar Falcão
Analytica Chimica Acta, 2013, Volume 768, Page 12
Graeme Eisenhofer, Arthur S. Tischler, and Ronald R. de Krijger
Endocrine Pathology, 2012, Volume 23, Number 1, Page 4

Comments (0)

Please log in or register to comment.
Log in