Jump to ContentJump to Main Navigation
Show Summary Details
In This Section

Clinical Chemistry and Laboratory Medicine (CCLM)

Published in Association with the European Federation of Clinical Chemistry and Laboratory Medicine (EFLM)

Editor-in-Chief: Plebani, Mario

Ed. by Gillery, Philippe / Lackner, Karl J. / Lippi, Giuseppe / Melichar, Bohuslav / Payne, Deborah A. / Schlattmann, Peter / Tate, Jillian R.

12 Issues per year


IMPACT FACTOR 2016: 3.432

CiteScore 2016: 2.21

SCImago Journal Rank (SJR) 2015: 0.873
Source Normalized Impact per Paper (SNIP) 2015: 0.982

Online
ISSN
1437-4331
See all formats and pricing
In This Section
Volume 49, Issue 9 (Sep 2011)

Issues

Development of candidate reference reagent for HIV-1 RNA and comparison analysis for different HIV-1 RNA quantitative assay

Borae G. Park
  • Department of Laboratory Medicine, University of Ulsan College of Medicine and Asan Medical Center, Seoul, Republic of Korea
  • Department of Laboratory Medicine, College of Medicine, Chung-Ang University, Seoul, Republic of Korea
/ Ae Ja Park
  • Department of Laboratory Medicine, College of Medicine, Chung-Ang University, Seoul, Republic of Korea
  • Email:
/ Jee-Hye Choi
  • Department of Laboratory Medicine, College of Medicine, Chung-Ang University, Seoul, Republic of Korea
  • Department of Life Science, College of Natural Science, Chung-Ang University, Seoul, Republic of Korea
/ Jina Park
  • Department of Laboratory Medicine, College of Medicine, Chung-Ang University, Seoul, Republic of Korea
  • Department of Life Science, College of Natural Science, Chung-Ang University, Seoul, Republic of Korea
/ Sung Soon Kim
  • Division of AIDS, Center for Immunology and Pathology, National Institute of Health, Korea Center for Disease Control and Prevention, Seoul, Republic of Korea
/ Jin-Sook Wang
  • Division of AIDS, Center for Immunology and Pathology, National Institute of Health, Korea Center for Disease Control and Prevention, Seoul, Republic of Korea
/ Mee Kyung Kee
  • Division of AIDS, Center for Immunology and Pathology, National Institute of Health, Korea Center for Disease Control and Prevention, Seoul, Republic of Korea
/ Ju-yeon Choi
  • Division of AIDS, Center for Immunology and Pathology, National Institute of Health, Korea Center for Disease Control and Prevention, Seoul, Republic of Korea
Published Online: 2011-06-11 | DOI: https://doi.org/10.1515/CCLM.2011.231

Abstract

Background: Human immunodeficiency virus type-1 (HIV-1) RNA viral load is a surrogate marker that is routinely used to determine indications for, and monitor the effectiveness of HIV-1 treatment. We developed three reagents for potential use in routine quality control of HIV-1 RNA quantitative assays. In this report, we compare the stability of these re-agents in storage and compare their performance in three different HIV-1 RNA quantitative assays.

Methods: The candidate reagents were derived from readily available pre-existing reagents and examined for stability at different storage temperatures. They were compared in three commercially available HIV-1 RNA quantitative assays: the Cobas TaqMan HIV-1 Test (Cobas TaqMan), the RealTime HIV-1 Assay (Abbott RealTime), and the NucliSens EasyQ HIV-1 Assay v1.1 (NucliSens EasyQ).

Results: The candidate reagent derived from an HIV culture supernatant (candidate CS) was the most stable of the three candidates and showed good reproducibility. Candidate CS yielded the highest HIV-1 titer of the three candidates in the Cobas TaqMan assay and the lowest HIV-1 titer and stability of the three candidates in the NucliSens EasyQ system.

Conclusions: The candidate CS is the most appropriate of the three candidate reagents for quantitative testing of HIV-1 RNA. This working reagent should be useful for use in routine calibration for quality control in centers with limited financial resources. The Cobas TaqMan assay tended to yield higher viral load results than the other assays when used with our three candidate reagents.

Keywords: HIV-1 RNA; quantitation; real-time PCR; stability; working reagent

About the article

Corresponding author: Ae Ja Park, MD, Department of Laboratory Medicine, College of Medicine, Chung-Ang University, 224-1, Heuksuk-Dong, Dongjak-Gu, Seoul 156-755, Republic of Korea Phone: +82 2 6299 2717, Fax: +82 2 6298 8630


Received: 2010-11-11

Accepted: 2011-04-05

Published Online: 2011-06-11

Published in Print: 2011-09-01



Citation Information: Clinical Chemistry and Laboratory Medicine, ISSN (Online) 1437-4331, ISSN (Print) 1434-6621, DOI: https://doi.org/10.1515/CCLM.2011.231. Export Citation

Citing Articles

Here you can find all Crossref-listed publications in which this article is cited. If you would like to receive automatic email messages as soon as this article is cited in other publications, simply activate the “Citation Alert” on the top of this page.

[1]
Bianca Bruzzone, Francesca Bisio, Patrizia Caligiuri, Franc A. Mayinda Mboungou, Nicola Nigro, Laura Sticchi, Agostina Ventura, Francesco Saladini, Maurizio Zazzi, Giancarlo Icardi, and Claudio Viscoli
Journal of Virological Methods, 2014, Volume 203, Page 102

Comments (0)

Please log in or register to comment.
Log in