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Clinical Chemistry and Laboratory Medicine (CCLM)

Published in Association with the European Federation of Clinical Chemistry and Laboratory Medicine (EFLM)

Editor-in-Chief: Plebani, Mario

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1437-4331
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Volume 50, Issue 1 (Jan 2012)

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Extracellular matrix-associated (GAGs, CTGF), angiogenic (VEGF) and inflammatory factors (MCP-1, CD40, IFN-γ) in type 1 diabetes mellitus nephropathy

Olga Ellina
  • Department of Experimental Physiology, Medical School, National and Kapodistrian University of Athens, Athens, Greece
/ Antonios Chatzigeorgiou
  • Department of Experimental Physiology, Medical School, National and Kapodistrian University of Athens, Athens, Greece
  • A. Chatzigeorgiou asserts equal contribution and joint first authorship.
/ Sophia Kouyanou
  • Division of Genetics and Biotechnology, Department of Biology, National and Kapodistrian University of Athens, Athens, Greece
/ Maria Lymberi
  • Department of Experimental Physiology, Medical School, National and Kapodistrian University of Athens, Athens, Greece
/ Christina Mylona-Karagianni
  • Second Department of Pediatrics, Medical School, National and Kapodistrian University of Athens, Panagiotis and Aglaia Kyriakou Pediatric Hospital, Athens, Greece
/ Emmanouil Tsouvalas
  • Second Department of Pediatrics, Medical School, National and Kapodistrian University of Athens, Panagiotis and Aglaia Kyriakou Pediatric Hospital, Athens, Greece
/ Elli F. Kamper
  • Department of Experimental Physiology, Medical School, National and Kapodistrian University of Athens, Athens, Greece
  • Email:

Abstract

Background: The aim of the present study was to evaluate the role of extracellular matrix-associated glycosaminoglycans (GAGs), connective tissue growth factor (CTGF), angiogenic vascular endothelial growth factor (VEGF) and inflammatory factors (MCP-1, CD40, IFN-γ) in the development of diabetic nephropathy in type 1 diabetes (T1DM).

Methods: Plasma and urine samples from 30 T1DM patients and 20 healthy controls were used to measure the levels of CTGF, VEGF, MCP-1, CD40 and IFN-γ by ELISA. Plasma and urine GAGs were measured using a spectrophotometric method.

Results: Plasma levels of GAGs, CD40 and MCP-1 and urine levels of GAGs and CTGF were significantly elevated in normoalbuminuric T1DM patients. A tendency to higher plasma VEGF levels was found in patients compared to controls. The urine/plasma GAGs ratio of T1DM patients was almost similar to that of healthy subjects (HS), whereas the urine/plasma CTGF ratio was about three times greater in diabetic patients compared to HS.

Conclusions: Conclusively, increased GAGs and CTGF excretion are evident in T1DM normoalbuminuric juveniles, possibly reflecting early renal injury signs, before the initiation of albuminuria.

Keywords: connective tissue growth factor; diabetic nephro­pathy (DN); extracellular matrix (ECM); glycosaminoglycans (GAGs); inflammation; type 1 diabetes mellitus (T1DM)

About the article

Corresponding author: Dr. Elli F. Kamper, Department of Experimental Physiology, Medical School, National and Kapodistrian University of Athens 75, M. Asias Str., GR-11527, Goudi, Athens, Greece Phone: +30-210-7462595, Fax: +30-210-7462594


Received: 2011-06-28

Accepted: 2011-09-06

Published in Print: 2012-01-01



Citation Information: Clinical Chemistry and Laboratory Medicine, ISSN (Online) 1437-4331, ISSN (Print) 1434-6621, DOI: https://doi.org/10.1515/cclm.2011.881. Export Citation

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