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Clinical Chemistry and Laboratory Medicine (CCLM)

Published in Association with the European Federation of Clinical Chemistry and Laboratory Medicine (EFLM)

Editor-in-Chief: Plebani, Mario

Ed. by Gillery, Philippe / Greaves, Ronda / Lackner, Karl J. / Lippi, Giuseppe / Melichar, Bohuslav / Payne, Deborah A. / Schlattmann, Peter


IMPACT FACTOR 2018: 3.638

CiteScore 2018: 2.44

SCImago Journal Rank (SJR) 2018: 1.191
Source Normalized Impact per Paper (SNIP) 2018: 1.205

Online
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1437-4331
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Volume 50, Issue 10

Issues

Long-term (in)stability of folate and vitamin B12 in human serum

Eugène H.J.M. Jansen
  • Corresponding author
  • Laboratory for Health Protection Research, National Institute of Public Health and the Environment, Bilthoven, The Netherlands
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/ Piet K. Beekhof
  • Laboratory for Health Protection Research, National Institute of Public Health and the Environment, Bilthoven, The Netherlands
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Johannes W.J.M. Cremers
  • Laboratory for Health Protection Research, National Institute of Public Health and the Environment, Bilthoven, The Netherlands
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  • De Gruyter OnlineGoogle Scholar
/ Erna Schenk
  • Laboratory for Health Protection Research, National Institute of Public Health and the Environment, Bilthoven, The Netherlands
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
Published Online: 2012-04-16 | DOI: https://doi.org/10.1515/cclm-2012-0108

Abstract

Background: In epidemiological research it is very important to test the stability of biomarkers as a function of both storage time and temperature. In this study the stability of both folate and vitamin B12 in human serum samples have been tested after storage at three different temperatures up to 1 year.

Methods: Serum samples of 16 individuals were used in this study. The concentration of folate and vitamin B12 has been determined at T=0 and at several time points up to 1 year after storage at –20°C, –70°C and –196°C. The statistical difference from the initial value at T=0 were determined with a t-test.

Results: Folate in serum samples remained stable at –70°C but was not stable during storage at –20°C. A fast decrease was observed after Day 4 which resulted in a stable level of about 60% of the original value measured at T=0 (p<0.001). The rank order of folate concentration in the samples, however, was not affected. The stability of vitamin B12 was good at all temperatures tested.

Conclusions: Measurements of folate concentrations in serum stored at –20°C are not reliable. The rank order, however, was not changed. Vitamin B12 was stable at all temperatures tested. For both folate and vitamin B12 storage at –70°C is sufficient to maintain the original concentration for 1 year. Storage at –196°C in liquid nitrogen is not necessary for these nutrients.

Keywords: folate; stability; storage; vitamin B12

About the article

Corresponding author: Eugène H.J.M. Jansen, Laboratory for Health Protection Research, National Institute of Public Health and the Environment, PO Box 1, 3720 BA Bilthoven, The Netherlands Phone: +31 302742940, Fax: +31 302744446


Received: 2012-02-21

Accepted: 2012-03-25

Published Online: 2012-04-16

Published in Print: 2012-10-01


Citation Information: Clinical Chemistry and Laboratory Medicine, Volume 50, Issue 10, Pages 1761–1763, ISSN (Online) 1437-4331, ISSN (Print) 1434-6621, DOI: https://doi.org/10.1515/cclm-2012-0108.

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