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Clinical Chemistry and Laboratory Medicine (CCLM)

Published in Association with the European Federation of Clinical Chemistry and Laboratory Medicine (EFLM)

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1437-4331
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Volume 50, Issue 2 (Feb 2012)

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A new dot immunoassay for simultaneous detection of celiac specific antibodies and IgA-deficiency

Karsten Conrad
  • Institute of Immunology, Technical University Dresden, Dresden, Germany
  • Karsten Conrad and Dirk Roggenbuck contributed equally.
/ Dirk Roggenbuck
  • GA Generic Assays GmbH, Dahlewitz, Germany
  • Karsten Conrad and Dirk Roggenbuck contributed equally.
/ Annelore Ittenson
  • Institute of Molecular and Clinical Immunology, Otto-von-Guericke University Magdeburg, Magdeburg, Germany
/ Dirk Reinhold
  • Institute of Molecular and Clinical Immunology, Otto-von-Guericke University Magdeburg, Magdeburg, Germany
/ Thomas Buettner
  • GA Generic Assays GmbH, Dahlewitz, Germany
/ Martin W. Laass
  • Children’s Hospital, Technical University Dresden, Dresden, Germany
  • Email:

Abstract

Background: This study investigated whether a dot immunoassay (DIA) can provide simultaneous detection of anti-tissue transglutaminase (tTG), anti-deamidated gliadin (DG) and total IgA antibodies, as required in the work-up of celiac disease (CD) patients.

Methods: Celiac disease patients (n=111) consecutively diagnosed from 2001 to 2011 at the Children’s Hospital and Institute of Immunology (Technical University Dresden) were tested for anti-tTG, anti-DG and total IgA by enzyme-linked immunosorbent assay (ELISA) and DIA retrospectively. Blood donors (n=45) and non-CD individuals with low IgA serum levels (n=8) were included as controls. Antibodies to endomysial antigens (EmA) were assessed by indirect immunofluorescence (IIF).

Results: Four (3.6%) of 111 CD patients demonstrated an IgA deficiency with total IgA below 50 mg/L by ELISA. Total IgA of the 107 IgA-non-deficient CD patients varied from 70 to 6000 mg/L. All four IgA-deficient CD patients were detected by a reduced reaction control of DIA and demonstrated positive anti-tTG or anti-DG IgG by DIA or ELISA. Detection of anti-tTG and anti-DG by DIA and ELISA showed a very good agreement (IgA: κ=0.972, 0.856, respectively; IgG: 0.921, 0.895, respectively).

Conclusions: Immunodot assay is a reliable and easy-to-use technique for the detection of IgA-deficient CD patients. Simultaneous assessment of anti-tTG and anti-DG IgA antibodies, and IgA deficiency by DIA can improve the efficacy of CD serology.

Keywords: anti-deamidated gliadin antibody; anti-transglutaminase antibody; celiac disease; IgA deficiency

About the article

Corresponding author: Dr. Martin W. Laass, Children’s Hospital, Technical University Dresden, 01307 Dresden, 74 Fetscherstraße, Germany Phone: +(49)-351-458 3449, Fax: +(49)-351-458 5865


Received: 2011-08-21

Accepted: 2011-09-29

Published in Print: 2012-02-01


Citation Information: Clinical Chemistry and Laboratory Medicine (CCLM), ISSN (Online) 1437-4331, ISSN (Print) 1434-6621, DOI: https://doi.org/10.1515/cclm.2011.760. Export Citation

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