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Clinical Chemistry and Laboratory Medicine (CCLM)

Published in Association with the European Federation of Clinical Chemistry and Laboratory Medicine (EFLM)

Editor-in-Chief: Plebani, Mario

Ed. by Gillery, Philippe / Lackner, Karl J. / Lippi, Giuseppe / Melichar, Bohuslav / Payne, Deborah A. / Schlattmann, Peter / Tate, Jillian R.

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1437-4331
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Volume 50, Issue 2 (Feb 2012)

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Quantitative performance of antibody array technology in a prenatal screening setting

Wendy Rodenburg
  • Laboratory for Health Protection Research, National Institute of Public Health and the Environment, Bilthoven, The Netherlands
/ Johan H. Reimerink
  • Laboratory for Infectious Diseases and Perinatal Screening, National Institute of Public Health and the Environment, Bilthoven, The Netherlands
/ Sandra Imholz
  • Laboratory for Health Protection Research, National Institute of Public Health and the Environment, Bilthoven, The Netherlands
/ Gert-Jan Godeke
  • Laboratory for Infectious Diseases and Perinatal Screening, National Institute of Public Health and the Environment, Bilthoven, The Netherlands
/ Jeroen L.A. Pennings
  • Laboratory for Health Protection Research, National Institute of Public Health and the Environment, Bilthoven, The Netherlands
/ Peter C.J.I. Schielen
  • Laboratory for Infectious Diseases and Perinatal Screening, National Institute of Public Health and the Environment, Bilthoven, The Netherlands
/ Maria P.H. Koster
  • Laboratory for Infectious Diseases and Perinatal Screening, National Institute of Public Health and the Environment, Bilthoven, The Netherlands
/ Annemieke de Vries
  • Laboratory for Health Protection Research, National Institute of Public Health and the Environment, Bilthoven, The Netherlands
  • Email:
Published Online: 2011-10-28 | DOI: https://doi.org/10.1515/cclm.2011.767

Abstract

Background: Antibody microarrays (Ab-array) represent a new, innovative proteomics platform for high-throughput protein expression profiling in body fluids. Because they allow for multiplexed measurements in small sample volumes, Ab-arrays are an interesting alternative to conventional indirect sandwich immunoassay (ELISA or DELFIA) tests in clinical or population screening if sets of markers are to be analyzed simultaneously. However, to allow implementation of Ab-arrays in clinical or population screening programs, it is of vital importance to establish that this method is both sensitive and quantitative.

Methods: This study developed and optimized a duplex Ab-array for pregnancy-associated plasma protein A (PAPP-A) and human chorion gonadotropin (fβ-hCG), two serum biomarkers currently analyzed by conventional biochemical techniques in prenatal screening. Serum samples from pregnant women, representing the dynamic range of both markers, were analyzed on Ab-arrays, and validated to the, in prenatal screening routinely applied, AutoDelfia system.

Results: Two different array hybridization conditions were tested, i.e., direct and indirect labeling, of which the indirect method displayed a sensitive and quantitative performance and a low intra- and inter-assay variation.

Conclusions: Taken together, these findings indicate that Ab-array technology is a promising alternative for ELISA or DELFIA in population screening programs, allowing future quantitative analysis of multiple biomarkers simultaneously in small volumes of serum.

This article offers supplementary material which is provided at the end of the article.

Keywords: antibody array; biomarker; prenatal screening; quantitative performance

About the article

Corresponding author: Dr. Annemieke de Vries, Laboratory for Health Protection Research, National Institute of Public Health and the Environment, PO Box 1, 3720 BA Bilthoven, The Netherlands Phone: +31 30 274 3483, Fax: +31 30 274 4446


Received: 2011-06-21

Accepted: 2011-10-03

Published Online: 2011-10-28

Published in Print: 2012-02-01


Citation Information: Clinical Chemistry and Laboratory Medicine (CCLM), ISSN (Online) 1437-4331, ISSN (Print) 1434-6621, DOI: https://doi.org/10.1515/cclm.2011.767.

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©2012 by Walter de Gruyter Berlin Boston. Copyright Clearance Center

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[1]
Jeroen L. A. Pennings, Sandra Imholz, Ilse Zutt, Maria P. H. Koster, Jacqueline E. Siljee, Annemieke de Vries, Peter C. J. I. Schielen, and Wendy Rodenburg
Disease Markers, 2015, Volume 2015, Page 1
[2]
Jeroen L. A. Pennings, Jacqueline E. Siljee, Sandra Imholz, Sylwia Kuc, Annemieke de Vries, Peter C. J. I. Schielen, and Wendy Rodenburg
Disease Markers, 2014, Volume 2014, Page 1

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