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Clinical Chemistry and Laboratory Medicine (CCLM)

Published in Association with the European Federation of Clinical Chemistry and Laboratory Medicine (EFLM)

Editor-in-Chief: Plebani, Mario

Ed. by Gillery, Philippe / Greaves, Ronda / Lackner, Karl J. / Lippi, Giuseppe / Melichar, Bohuslav / Payne, Deborah A. / Schlattmann, Peter

IMPACT FACTOR 2018: 3.638

CiteScore 2018: 2.44

SCImago Journal Rank (SJR) 2018: 1.191
Source Normalized Impact per Paper (SNIP) 2018: 1.205

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Volume 50, Issue 6


Evaluation of Prevent ID and Quantum Blue rapid tests for fecal calprotectin

Jan Hessels / Geke Douw / Duygu D. Yildirim / Gerrit Meerman / Margot A. van Herwaarden / Frank A.J.T.M. van den Bergh
  • Laboratory for Clinical Chemistry, Hospital Medisch Spectrum Twente, Enschede, The Netherlands
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
Published Online: 2012-01-13 | DOI: https://doi.org/10.1515/cclm-2011-0855


Background: Tests for fecal calprotectin are usually either enzyme-linked immunosorbent assays (ELISA) or a time-resolved fluorimetric immunoassay (TRFIA). These time-consuming tests are performed only once every 1 or 2 weeks. Before the results of the tests are known most patients have already undergone colonoscopy. A rapid test, performed on outpatients, could minimize the number of necessary colonoscopies. To establish optimal cut-off values minimizing the necessity for colonoscopies, we compared two commercially available rapid tests with a quantitative TRFIA.

Methods: Fecal samples were collected from 85 patients with lower gastrointestinal complaints. Calprotectin was measured using quantitative TRFIA as well as using two rapid tests: Prevent ID CalDetect and Quantum Blue calprotectin. We used the TRFIA method as the golden standard with a cut-off value of 50 μg/g. The percentage correct classification, sensitivity, specificity and positive and negative predictive value were calculated for both rapid tests at various cut-off levels.

Results: Correlation between both of the rapid tests with TRFIA was significant. Quantum Blue calprotectin (κ 0.77) correlated better than Prevent ID CalDetect (κ 0.46). Optimal cut-off levels for Prevent ID CalDetect and Quantum Blue calprotectin rapid tests were 15 μg/g and 40 μg/g with a reduction in the number of necessary colonoscopies of 39% and 62%, respectively.

Conclusions: The Quantum Blue calprotectin rapid test demonstrated better analytical performance than the Prevent ID CalDetect in reducing the number of colonoscopies. Furthermore, the former test has the advantage of using a point of care reader for quantitative measurement and for establishing an optimal cut-off level.

Keywords: calprotectin; fecal; rapid test; time-resolved fluorimetric immunoassay

About the article

Corresponding author: Dr. Jan Hessels, Clinical Chemist, Laboratory for Clinical Chemistry, Deventer Ziekenhuis, Nico Bolkesteinlaan 75, 7416 SE Deventer, The Netherlands Phone: +31 570 535031, Fax: +31 570 501428

Received: 2011-08-04

Accepted: 2011-12-14

Published Online: 2012-01-13

Published in Print: 2012-06-01

Citation Information: Clinical Chemistry and Laboratory Medicine, Volume 50, Issue 6, Pages 1079–1082, ISSN (Online) 1437-4331, ISSN (Print) 1434-6621, DOI: https://doi.org/10.1515/cclm-2011-0855.

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