Jump to ContentJump to Main Navigation
Show Summary Details
More options …

Clinical Chemistry and Laboratory Medicine (CCLM)

Published in Association with the European Federation of Clinical Chemistry and Laboratory Medicine (EFLM)

Editor-in-Chief: Plebani, Mario

Ed. by Gillery, Philippe / Lackner, Karl J. / Lippi, Giuseppe / Melichar, Bohuslav / Payne, Deborah A. / Schlattmann, Peter / Tate, Jillian R.

12 Issues per year


IMPACT FACTOR 2016: 3.432

CiteScore 2016: 2.21

SCImago Journal Rank (SJR) 2016: 1.000
Source Normalized Impact per Paper (SNIP) 2016: 1.112

Online
ISSN
1437-4331
See all formats and pricing
More options …
Volume 50, Issue 9 (Sep 2012)

Issues

Interference of the new oral anticoagulant dabigatran with frequently used coagulation tests

Walter-Michael Halbmayer
  • Corresponding author
  • Institute of Laboratory Medicine, Municipal Hospital Hietzing-Rosenhuegel, Vienna, Austria
  • WMH and GW equally contributed to this work.
  • Email
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Guenter Weigel
  • Central Institute of Medical and Chemical Laboratory Diagnostics, University Hospital – State Hospital Innsbruck, Innsbruck, Austria
  • WMH and GW equally contributed to this work.
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Peter Quehenberger / Josef Tomasits / Alexander C. Haushofer / Gerold Aspoeck / Lorin Loacker
  • Central Institute of Medical and Chemical Laboratory Diagnostics, University Hospital – State Hospital Innsbruck, Innsbruck, Austria
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Mirjam Schnapka-Koepf
  • Central Institute of Medical and Chemical Laboratory Diagnostics, University Hospital – State Hospital Innsbruck, Innsbruck, Austria
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Georg Goebel
  • Department for Medical Statistics, Informatics and Health Economics, Medical University of Innsbruck, Innsbruck, Austria
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Andrea Griesmacher
  • Central Institute of Medical and Chemical Laboratory Diagnostics, University Hospital – State Hospital Innsbruck, Innsbruck, Austria
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
Published Online: 2012-03-16 | DOI: https://doi.org/10.1515/cclm-2011-0888

Abstract

Background: Dabigatran etexilate is a new oral anticoagulant for the therapy and prophylaxis of venous thromboembolism and stroke prevention in patients with atrial fibrillation. To investigate the extent of interactions of this new anticoagulant with frequently used coagulation assays, we completed a multicenter in vitro trial with Conformité Européenne(CE)-labeled dabigatran-spiked plasma samples.

Methods: Lyophilized plasma samples with dabigatran concentrations ranging from 0.00 to 0.48 μg/mL were sent to the coagulation laboratories of six major Austrian hospitals for evaluation. Coagulation assays were performed under routine conditions using standard reagents and analyzer.

Results: Dabigatran led to a dose-dependent prolongation of the clotting times in coagulometric tests and influenced the majority of the parameters measured. Statistically significant interference could be observed with the prothrombin time (PT), activated partial thromboplastin time (aPTT) and PT/aPTT-based assays (extrinsic/intrinsic factors, APC-resistance test) as well as lupus anticoagulant testing. Even non-clotting tests, such as the colorimetric factor XIII activity assay and to a minor extent the amidolytic antithrombin activity assay (via factor IIa) were affected.

Conclusions: This multicenter trial confirms and also adds to existing data, demonstrating that laboratories should expect to observe strong interferences of coagulation tests with increasing concentrations of dabigatran. This finding might become particularly important in the elderly and in patients with renal impairment as well as patients whose blood is drawn at peak levels of dabigatran.

Keywords: coagulation tests; dabigatran; interference; misinterpretation; new oral anticoagulants

About the article

Corresponding author: Univ.-Doz. Dr. Walter-Michael Halbmayer, Institute of Laboratory Medicine, Municipal Hospital Hietzing-Rosenhuegel, Wolkersbergenstr. 1, 1130 Vienna, Austria Phone: +43 180110-2088, Fax: +43 180110-2688


Received: 2011-11-28

Accepted: 2012-02-06

Published Online: 2012-03-16

Published in Print: 2012-09-01


Citation Information: Clinical Chemistry and Laboratory Medicine (CCLM), ISSN (Online) 1437-4331, ISSN (Print) 1434-6621, DOI: https://doi.org/10.1515/cclm-2011-0888.

Export Citation

©2012 by Walter de Gruyter Berlin Boston. Copyright Clearance Center

Citing Articles

Here you can find all Crossref-listed publications in which this article is cited. If you would like to receive automatic email messages as soon as this article is cited in other publications, simply activate the “Citation Alert” on the top of this page.

[1]
A. Antovic, E. Norberg, M. Berndtsson, A. Rasmuson, R. E. Malmström, M. Skeppholm, and J. Antovic
Thrombosis and Haemostasis, 2017, Volume 117, Number 9, Page 1700
[2]
Elias Kyriakou, Ignatios Ikonomidis, Dimitrios Stylos, Stefanos Bonovas, Ioannis Papadakis, Georgios K. Nikolopoulos, Styliani Kokoris, Dimitrios Kalantzis, Christine Economopoulou, Petros Kopterides, John Lekakis, and Argirios E. Tsantes
Clinical and Applied Thrombosis/Hemostasis, 2015, Volume 21, Number 5, Page 434
[3]
Sebastian Boehr and Ekkehard Haen
Therapeutic Drug Monitoring, 2017, Volume 39, Number 1, Page 66
[4]
Bethany T. Samuelson, Adam Cuker, Deborah M. Siegal, Mark Crowther, and David A. Garcia
Chest, 2017, Volume 151, Number 1, Page 127
[5]
Ariela Hoxha, Alessandra Banzato, Amelia Ruffatti, and Vittorio Pengo
Autoimmunity Reviews, 2017, Volume 16, Number 2, Page 173
[6]
Savino Sciascia, Chary Lopez-Pedrera, Irene Cecchi, Clara Pecoraro, Dario Roccatello, and Maria Josè Cuadrado
Rheumatology, 2016, Volume 55, Number 10, Page 1726
[7]
B. Schenk, P. Würtinger, W. Streif, W. Sturm, D. Fries, M. Bachler, and H. C. Hemmings
British Journal of Anaesthesia, 2016, Volume 117, Number 5, Page 583
[8]
F. Ratzinger, M. Lang, S. Belik, P. Jilma-Stohlawetz, K. G. Schmetterer, H. Haslacher, T. Perkmann, and P. Quehenberger
Thrombosis and Haemostasis, 2016, Volume 116, Number 2, Page 235
[9]
Yuta Fujimori, Masatoshi Wakui, Hisako Katagiri, Kentaro Ohira, Nobuko Shimizu, and Mitsuru Murata
Journal of Clinical Pathology, 2016, Volume 69, Number 4, Page 370
[10]
Alina Scridon and Răzvan C. Şerban
Therapeutic Drug Monitoring, 2016, Volume 38, Number 1, Page 12
[11]
Roslyn Bonar, Emmanuel J. Favaloro, Soma Mohammed, leonardo pasalic, John Sioufi, and Katherine Marsden
Pathology, 2015, Volume 47, Number 4, Page 355
[12]
Paul K. L. Chin, David M. Patterson, Mei Zhang, Berit P. Jensen, Daniel F. B. Wright, Murray L. Barclay, and Evan J. Begg
British Journal of Clinical Pharmacology, 2014, Volume 78, Number 3, Page 630
[13]
B. A. Konkle
Hematology, 2014, Volume 2014, Number 1, Page 329
[14]
Ingrid Pabinger, Wilfried Lang, Franz Xaver Roithinger, Franz Weidinger, Sabine Eichinger-Hasenauer, Reinhold Glehr, Walter-Michael Halbmayer, Hans-Peter Haring, Bernd Jilma, Hans Christian Korninger, Sibylle Kozek-Langenecker, Paul Kyrle, Herbert Watzke, Ansgar Weltermann, Johann Willeit, and Kurt Huber
Wiener klinische Wochenschrift, 2014, Volume 126, Number 23-24, Page 792
[15]
M. Van Blerk, E. Bailleul, B. Chatelain, A. Demulder, K. Devreese, J. Douxfils, K. Jochmans, F. Mullier, W. Wijns, M. R. Soumali, W. Coucke, K. Vernelen, and P. Van de Walle
Thrombosis and Haemostasis, 2014, Volume 113, Number 1, Page 154
[16]
Adam Cuker, Deborah M. Siegal, Mark A. Crowther, and David A. Garcia
Journal of the American College of Cardiology, 2014, Volume 64, Number 11, Page 1128
[17]
A. Hillarp, K. M. Gustafsson, L. Faxälv, K. Strandberg, F. Baghaei, I. Fagerberg Blixter, M. Berndtsson, and T. L. Lindahl
Journal of Thrombosis and Haemostasis, 2014, Volume 12, Number 9, Page 1545
[18]
Steve Kitchen, Elaine Gray, Ian Mackie, Trevor Baglin, and Mike Makris
British Journal of Haematology, 2014, Volume 166, Number 6, Page 830
[19]
Sibylle A. Kozek-Langenecker
Seminars in Hematology, 2014, Volume 51, Number 2, Page 112
[20]
Dietmar Fries, Alexander Giurea, Manfred Gütl, Walter-Michael Halbmayer, Sibylle Kozek-Langenecker, Andreas Pachucki, Franz Roithinger, Barbara Steinlechner, Heinrich Thaler, and Ansgar Weltermann
Wiener klinische Wochenschrift, 2013, Volume 125, Number 21-22, Page 721
[21]
M. E. Martinuzzo, L. H. Barrera, M. A. D 'Adamo, J. C. Otaso, M. I. Gimenez, and J. Oyhamburu
International Journal of Laboratory Hematology, 2014, Volume 36, Number 2, Page 144
[22]
G. Hapgood, J. Butler, E. Malan, S. Chunilal, and H. Tran
Thrombosis and Haemostasis, 2013, Volume 110, Number 2, Page 308
[23]
A. Tripodi
Blood, 2013, Volume 121, Number 20, Page 4032
[24]
Wilson I. Gonsalves, Rajiv K. Pruthi, and Mrinal M. Patnaik
Mayo Clinic Proceedings, 2013, Volume 88, Number 5, Page 495
[25]
C. Eby
International Journal of Laboratory Hematology, 2013, Volume 35, Number 3, Page 262

Comments (0)

Please log in or register to comment.
Log in