Jump to ContentJump to Main Navigation
Show Summary Details

Clinical Chemistry and Laboratory Medicine (CCLM)

Published in Association with the European Federation of Clinical Chemistry and Laboratory Medicine (EFLM)

Editor-in-Chief: Plebani, Mario

Ed. by Gillery, Philippe / Lackner, Karl J. / Lippi, Giuseppe / Melichar, Bohuslav / Payne, Deborah A. / Schlattmann, Peter / Tate, Jillian R.

IMPACT FACTOR increased in 2015: 3.017
Rank 5 out of 30 in category Medical Laboratory Technology in the 2014 Thomson Reuters Journal Citation Report/Science Edition

SCImago Journal Rank (SJR) 2015: 0.873
Source Normalized Impact per Paper (SNIP) 2015: 0.982
Impact per Publication (IPP) 2015: 2.238

See all formats and pricing


Select Volume and Issue


Interference of the new oral anticoagulant dabigatran with frequently used coagulation tests

1, a / Guenter Weigel2, a / Peter Quehenberger3 / Josef Tomasits4 / Alexander C. Haushofer5 / Gerold Aspoeck6 / Lorin Loacker2 / Mirjam Schnapka-Koepf2 / Georg Goebel7 / Andrea Griesmacher2

1Institute of Laboratory Medicine, Municipal Hospital Hietzing-Rosenhuegel, Vienna, Austria

2Central Institute of Medical and Chemical Laboratory Diagnostics, University Hospital – State Hospital Innsbruck, Innsbruck, Austria

3Clinical Institute of Laboratory Medicine, Medical University Vienna, Vienna, Austria

4Central Laboratory, General Hospital Linz, Linz, Austria

5Central Laboratory, Clinic St. Poelten, St. Poelten, Austria

6Institute of Laboratory Medicine I, Clinic Wels-Grieskirchen, Wels-Grieskirchen, Austria

7Department for Medical Statistics, Informatics and Health Economics, Medical University of Innsbruck, Innsbruck, Austria

aWMH and GW equally contributed to this work.

Corresponding author: Univ.-Doz. Dr. Walter-Michael Halbmayer, Institute of Laboratory Medicine, Municipal Hospital Hietzing-Rosenhuegel, Wolkersbergenstr. 1, 1130 Vienna, Austria Phone: +43 180110-2088, Fax: +43 180110-2688

Citation Information: Clinical Chemistry and Laboratory Medicine (CCLM). Volume 50, Issue 9, Pages 1601–1605, ISSN (Online) 1437-4331, ISSN (Print) 1434-6621, DOI: https://doi.org/10.1515/cclm-2011-0888, March 2012

Publication History

Published Online:


Background: Dabigatran etexilate is a new oral anticoagulant for the therapy and prophylaxis of venous thromboembolism and stroke prevention in patients with atrial fibrillation. To investigate the extent of interactions of this new anticoagulant with frequently used coagulation assays, we completed a multicenter in vitro trial with Conformité Européenne(CE)-labeled dabigatran-spiked plasma samples.

Methods: Lyophilized plasma samples with dabigatran concentrations ranging from 0.00 to 0.48 μg/mL were sent to the coagulation laboratories of six major Austrian hospitals for evaluation. Coagulation assays were performed under routine conditions using standard reagents and analyzer.

Results: Dabigatran led to a dose-dependent prolongation of the clotting times in coagulometric tests and influenced the majority of the parameters measured. Statistically significant interference could be observed with the prothrombin time (PT), activated partial thromboplastin time (aPTT) and PT/aPTT-based assays (extrinsic/intrinsic factors, APC-resistance test) as well as lupus anticoagulant testing. Even non-clotting tests, such as the colorimetric factor XIII activity assay and to a minor extent the amidolytic antithrombin activity assay (via factor IIa) were affected.

Conclusions: This multicenter trial confirms and also adds to existing data, demonstrating that laboratories should expect to observe strong interferences of coagulation tests with increasing concentrations of dabigatran. This finding might become particularly important in the elderly and in patients with renal impairment as well as patients whose blood is drawn at peak levels of dabigatran.

Keywords: coagulation tests; dabigatran; interference; misinterpretation; new oral anticoagulants

Citing Articles

Here you can find all Crossref-listed publications in which this article is cited. If you would like to receive automatic email messages as soon as this article is cited in other publications, simply activate the “Citation Alert” on the top of this page.

Alina Scridon and Răzvan C. Şerban
Therapeutic Drug Monitoring, 2016, Volume 38, Number 1, Page 12
Roslyn Bonar, Emmanuel J. Favaloro, Soma Mohammed, Leonardo Pasalic, John Sioufi, and Katherine Marsden
Pathology, 2015, Page 1
Paul K. L. Chin, David M. Patterson, Mei Zhang, Berit P. Jensen, Daniel F. B. Wright, Murray L. Barclay, and Evan J. Begg
British Journal of Clinical Pharmacology, 2014, Volume 78, Number 3, Page 630
B. A. Konkle
Hematology, 2014, Volume 2014, Number 1, Page 329
Ingrid Pabinger, Wilfried Lang, Franz Xaver Roithinger, Franz Weidinger, Sabine Eichinger-Hasenauer, Reinhold Glehr, Walter-Michael Halbmayer, Hans-Peter Haring, Bernd Jilma, Hans Christian Korninger, Sibylle Kozek-Langenecker, Paul Kyrle, Herbert Watzke, Ansgar Weltermann, Johann Willeit, and Kurt Huber
Wiener klinische Wochenschrift, 2014, Volume 126, Number 23-24, Page 792
M. Van Blerk, E. Bailleul, B. Chatelain, A. Demulder, K. Devreese, J. Douxfils, K. Jochmans, F. Mullier, W. Wijns, M. R. Soumali, W. Coucke, K. Vernelen, and P. Van de Walle
Thrombosis and Haemostasis, 2014, Volume 113, Number 1, Page 154
Adam Cuker, Deborah M. Siegal, Mark A. Crowther, and David A. Garcia
Journal of the American College of Cardiology, 2014, Volume 64, Number 11, Page 1128
A. Hillarp, K. M. Gustafsson, L. Faxälv, K. Strandberg, F. Baghaei, I. Fagerberg Blixter, M. Berndtsson, and T. L. Lindahl
Journal of Thrombosis and Haemostasis, 2014, Volume 12, Number 9, Page 1545
Sibylle A. Kozek-Langenecker
Seminars in Hematology, 2014, Volume 51, Number 2, Page 112
Dietmar Fries, Alexander Giurea, Manfred Gütl, Walter-Michael Halbmayer, Sibylle Kozek-Langenecker, Andreas Pachucki, Franz Roithinger, Barbara Steinlechner, Heinrich Thaler, and Ansgar Weltermann
Wiener klinische Wochenschrift, 2013, Volume 125, Number 21-22, Page 721
M. E. Martinuzzo, L. H. Barrera, M. A. D 'Adamo, J. C. Otaso, M. I. Gimenez, and J. Oyhamburu
International Journal of Laboratory Hematology, 2014, Volume 36, Number 2, Page 144
G. Hapgood, J. Butler, E. Malan, S. Chunilal, and H. Tran
Thrombosis and Haemostasis, 2013, Volume 110, Number 2, Page 308
A. Tripodi
Blood, 2013, Volume 121, Number 20, Page 4032
Wilson I. Gonsalves, Rajiv K. Pruthi, and Mrinal M. Patnaik
Mayo Clinic Proceedings, 2013, Volume 88, Number 5, Page 495
C. Eby
International Journal of Laboratory Hematology, 2013, Volume 35, Number 3, Page 262

Comments (0)

Please log in or register to comment.