Jump to ContentJump to Main Navigation
Show Summary Details
More options …

Clinical Chemistry and Laboratory Medicine (CCLM)

Published in Association with the European Federation of Clinical Chemistry and Laboratory Medicine (EFLM)

Editor-in-Chief: Plebani, Mario

Ed. by Gillery, Philippe / Lackner, Karl J. / Lippi, Giuseppe / Melichar, Bohuslav / Payne, Deborah A. / Schlattmann, Peter / Tate, Jillian R.

12 Issues per year


IMPACT FACTOR 2016: 3.432

CiteScore 2017: 2.34

SCImago Journal Rank (SJR) 2017: 1.114
Source Normalized Impact per Paper (SNIP) 2017: 1.188

Online
ISSN
1437-4331
See all formats and pricing
Online
Institutional Subscription
€ [D] 1975.00 / US$ 2960.00 / GBP 1619.00*
Individual Subscription
€ [D] 249.00 / US$ 374.00 / GBP 205.00*
Print
Institutional Subscription
€ [D] 1975.00 / US$ 2960.00 / GBP 1619.00*
Individual Subscription
€ [D] 1975.00 / US$ 2960.00 / GBP 1619.00*
Print + Online
Institutional Subscription
€ [D] 2370.00 / US$ 3551.00 / GBP 1943.00*
Individual Subscription
€ [D] 2370.00 / US$ 3551.00 / GBP 1943.00*
*Prices in US$ apply to orders placed in the Americas only. Prices in GBP apply to orders placed in Great Britain only. Prices in € represent the retail prices valid in Germany (unless otherwise indicated). Prices are subject to change without notice. Prices do not include postage and handling if applicable. RRP: Recommended Retail Price.
More options …
Volume 51, Issue 11

Issues

Volume 56 (2018)

Volume 55 (2017)

Volume 54 (2016)

Volume 53 (2015)

Volume 52 (2014)

Volume 51 (2013)

Volume 50 (2012)

Volume 49 (2011)

Volume 48 (2010)

Volume 47 (2009)

Volume 46 (2008)

Volume 45 (2007)

Volume 44 (2006)

Volume 43 (2005)

Volume 42 (2004)

Volume 41 (2003)

Volume 40 (2002)

Volume 39 (2001)

Volume 38 (2000)

Volume 37 (1999)

Volume 36 (1998)

Volume 35 (1997)

Volume 34 (1996)

Volume 33 (1995)

Volume 32 (1994)

Volume 31 (1993)

Volume 30 (1992)

Volume 29 (1991)

Volume 28 (1990)

Volume 27 (1989)

Volume 26 (1988)

Volume 25 (1987)

Volume 24 (1986)

Volume 23 (1985)

Volume 22 (1984)

Volume 21 (1983)

Volume 20 (1982)

Volume 19 (1981)

Volume 18 (1980)

Volume 17 (1979)

Volume 16 (1978)

Volume 15 (1977)

Volume 14 (1976)

Volume 13 (1975)

Volume 12 (1974)

Volume 11 (1973)

Volume 10 (1972)

Volume 9 (1971)

Volume 8 (1970)

Volume 7 (1969)

Volume 6 (1968)

Volume 5 (1967)

Volume 4 (1966)

Volume 3 (1965)

Volume 2 (1964)

Volume 1 (1963)

Influence of physical properties of cuvette surface on measurement of serum lipase

Lien Cattoir / Valerie Pede / An-Sofie Decavele / Marc L. De Buyzere / Marijn M. Speeckaert / David Seccombe / Veerle Boterberg / Peter Dubruel / Frank de Bisschop / Joris R. Delanghe
Published Online: 2013-07-10 | DOI: https://doi.org/10.1515/cclm-2013-0369

30,00 € / $42.00 / £23.00

Get Access to Full Text

Abstract

Background: Despite striking similarities among colorimetric lipase assay recipes, marked intervendor differences are noted in the reported lipase values. In the present study, the effect of physical properties of the cuvette surface on measurement of serum lipase was investigated.

Methods: Lipase activity was measured concomitantly in cuvettes from three different analyzers: Vista (Siemens), Modular (Roche), and Synchron (Beckman Coulter). The surface/volume ratio of the cuvettes and the contact angle of the cuvette polymers were determined. The effects of various characteristics of serum (biochemical parameters, surface tension) were also examined.

Results: Serum lipase activities based on the colorimetric methylresorufin assay differed markedly according to the cuvettes used. More specifically, in the lower activity rate, marked differences were reported. The physical properties of the various cuvettes showed remarkable differences, especially in the contact angles. Other biochemical parameters (bilirubin, alkaline phosphatase, total cholesterol, high-density lipoprotein cholesterol, low-density lipoprotein cholesterol, and triglycerides) and serum surface tension did not affect the results.

Conclusions: Serum lipase activity is affected by the physical properties of the cuvette surface.

Keywords: colorimetric method; cuvette; lipase; serum

References

  • 1.

    Lott JA, Patel ST, Sawhney AK, Kazmierczak SC, Love JE Jr. Assays of serum lipase: analytical and chemical considerations. Clin Chem 1986;32:1290–302.Google Scholar

  • 2.

    Lott JA, Lu CJ. Lipase isoforms and amylase isoenzymes: assays and application in the diagnosis of acute pancreatitis. Clin Chem 1991;37:361–8.Google Scholar

  • 3.

    Tietz NW, Shuey DF. Lipase in serum – the elusive enzyme: an overview. Clin Chem 1993;39:746–56.Google Scholar

  • 4.

    Gilham D, Lehner R. Techniques to measure lipase and esterase activity in vitro. Methods 2005;36:139–47.Google Scholar

  • 5.

    Sarda L, Desnuelle P. Action de la lipase pancréatique sur les esters en émulsion. Biochim Biophys Acta 1958;30:513–21.Google Scholar

  • 6.

    Predotova M, Kretschmann R, Gebauer J, Buerkert A. Effects of cuvette surface material on ammonia-, nitrous oxide-, carbon dioxide-, and methane-concentration measurements. J Plant Nutr Soil Sci 2011;174:347–9.Web of ScienceGoogle Scholar

  • 7.

    Available from www.google.com/patents/US4988497. Accessed May 15, 2013.

  • 8.

    Friedewald WT, Levy RI, Frederickson DS. Estimation of the concentration of low-density lipoprotein cholesterol in plasma, without use of the preparative ultracentrifuge. Clin Chem 1972;18:499–502.Google Scholar

  • 9.

    De Bisschop FR, Rigole WJ. A physical model for liquid capillary bridges between adsorptive solid spheres: the nodoid of plateau. J Colloid Interf Sci 1982;88:117–28.Google Scholar

  • 10.

    Rosina J, Kvasnak E, Suta D, Kolarova H, Malek J, Krajci L. Temperature dependence of blood surface tension. Physiol Res 2007;56:S93–8.Google Scholar

  • 11.

    Hrncír E, Rosina J. Surface tension of blood. Physiol Res 1997;46:319–21.Google Scholar

  • 12.

    Suttiprasit P, Krisdhasima V, McGuire J. The surface activity of α-lactalbumin, β-lactoglobulin, and bovine serum albumin: I. Surface tension measurements with single-component and mixed solutions. J Colloid Interf Sci 1992;154:316–26.Google Scholar

  • 13.

    Gutnick DL, Bach H. Biosurfactants. Comprehensive biotechnology, 2nd ed. 2011;3:699–715.Google Scholar

About the article

Corresponding author: Joris R. Delanghe, Department of Clinical Chemistry, De Pintelaan 185, 9000 Gent, Belgium, Phone: +32-9-332-2956, Fax: +32-9-332-4985, E-mail:

Received: 2013-05-16

Accepted: 2013-06-13

Published Online: 2013-07-10

Published in Print: 2013-11-01

Citation Information: Clinical Chemistry and Laboratory Medicine, Volume 51, Issue 11, Pages 2109–2114, ISSN (Online) 1437-4331, ISSN (Print) 1434-6621, DOI: https://doi.org/10.1515/cclm-2013-0369.

Export Citation

©2013 by Walter de Gruyter Berlin Boston.Get Permission

Citing Articles

Here you can find all Crossref-listed publications in which this article is cited. If you would like to receive automatic email messages as soon as this article is cited in other publications, simply activate the “Citation Alert” on the top of this page.

[1]
Jan-Hendrik Grosch, David Wagner, Niklas Knaup, Timm Keil, and Antje C. Spieß
Biotechnology Progress, 2017, Volume 33, Number 1, Page 87

Comments (0)

Please log in or register to comment.
Log in