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Clinical Chemistry and Laboratory Medicine (CCLM)

Published in Association with the European Federation of Clinical Chemistry and Laboratory Medicine (EFLM)

Editor-in-Chief: Plebani, Mario

Ed. by Gillery, Philippe / Greaves, Ronda / Lackner, Karl J. / Lippi, Giuseppe / Melichar, Bohuslav / Payne, Deborah A. / Schlattmann, Peter


IMPACT FACTOR 2018: 3.638

CiteScore 2018: 2.44

SCImago Journal Rank (SJR) 2018: 1.191
Source Normalized Impact per Paper (SNIP) 2018: 1.205

Online
ISSN
1437-4331
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Volume 51, Issue 6

Issues

Analysis of serous body fluids using the CELL-DYN Sapphire hematology analyzer

Jeffrey F.W. Keuren
  • Department of Clinical Chemistry and Hematology, Atrium Medical Center, Heerlen, the Netherlands
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Johannes J.M.L. Hoffmann / Mathie P.G. Leers
  • Corresponding author
  • Department of Clinical Chemistry and Hematology, Atrium Medical Center, Heerlen, the Netherlands
  • Email
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
Published Online: 2012-12-15 | DOI: https://doi.org/10.1515/cclm-2012-0549

Abstract

Background: Correct cell enumeration and differential analysis of body fluids are important in the diagnosis and management of several diseases. Currently, microscopic analysis is still considered the “gold standard”. The aim of the present study was to evaluate the analytical performance of the CELL-DYN Sapphire hematology analyzer for automated differentiation of cells in serous fluids and to explore whether manual analysis of the raw data files could improve the differential count compared with reference microscopy.

Methods: A total of 105 serous fluids (39 peritoneal and 66 pleural effusions) were analyzed by the CELL-DYN Sapphire using standard whole-blood algorithm. Additionally, we performed optimized manual gating of the Sapphire raw data file using standard flow cytometry software.

Results: The standard Sapphire algorithm showed substantial deviations from the reference microscopic differentiation: polymorphonuclear cell counts were too high because they contained some monocytic cells. However, when optimized manual gating strategy is used, a good correlation and negligible bias were found.

Conclusions: We have demonstrated that with a modified algorithm, CELL-DYN Sapphire will provide reliable identification and enumeration of blood cells in peritoneal and pleural fluids

Keywords: pleural effusion; mesothelial cells; microscopy

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About the article

Corresponding author: Mathie P.G. Leers, PhD, Department of Clinical Chemistry and Hematology, Atrium Medical Center, Henri Dunantstraat 5, 6401 CX Heerlen, the Netherlands, Phone: +31-45-576-7503, Fax: +31-45-576-6575


Received: 2012-08-28

Accepted: 2012-11-19

Published Online: 2012-12-15

Published in Print: 2013-06-01


Citation Information: Clinical Chemistry and Laboratory Medicine, Volume 51, Issue 6, Pages 1285–1290, ISSN (Online) 1437-4331, ISSN (Print) 1434-6621, DOI: https://doi.org/10.1515/cclm-2012-0549.

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