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Licensed Unlicensed Requires Authentication Published by De Gruyter December 25, 2012

A multiplex assay to rapidly exclude HLA-DQ2.5 and HLA-DQ8 expression in patients at risk for celiac disease

  • Ellen M. van Beek EMAIL logo , Elianne A. Roelandse-Koop , Raymon Vijzelaar , Rizkat Yilmaz , Ingrid M.W. van Hoogstraten , Marco W.J. Schreurs , Alice A.M. Verheul , Arend Jan van Houte and Wouter Kortlandt

Abstract

Background: Celiac disease (CD) is an inflammatory disorder of the small intestine induced by gluten ingestion. CD has a strong genetic association with human leukocyte antigen (HLA)-DQ2.5 and HLA-DQ8. The absence of HLA-DQ2.5 and HLA-DQ8 has a strong negative predictive value for CD. Genetic screening of HLA-DQ2.5 and HLA-DQ8 in patients at risk is of great value.

Methods: We designed, developed, and validated a multiplex assay based on multiplex ligation-dependent probe amplification (MLPA) technology, allowing the simultaneous detection of DQA1*05-DQB1*02, encoding HLA-DQ2.5, and DQA1*03-DQB1*03:02, encoding HLA-DQ8. The amplified products were separated and identified using capillary electrophoresis.

Results: When compared with a polymerase chain reaction followed by single-strand conformation polymorphism/ heteroduplex analysis, one discrepancy was found. Sequencing analysis showed that the developed MLPA assay result was correct. Furthermore, we demonstrated that the MLPA method is able to distinguish between the heterozygote and homozygote expression of HLA-DQ2.5 or HLA-DQ8.

Conclusions: This study shows that it is possible to rapidly and accurately screen for the absence of HLA-DQ2.5 and HLA-DQ8 using MLPA, excluding patients at risk for CD for further serological or histological follow-up. In addition, MLPA might be an accurate tool to screen for other specific HLA types in the context of disease association in a diagnostic laboratory setting.


Corresponding author: Ellen M. van Beek, Department of Clinical Chemistry, Hematology and Immunology, Diakonessenhuis Utrecht, Bosboomstraat 1, 3582 KE Utrecht, The Netherlands

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Received: 2012-09-27
Accepted: 2012-11-17
Published Online: 2012-12-25
Published in Print: 2013-06-01

©2013 by Walter de Gruyter Berlin Boston

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