Jump to ContentJump to Main Navigation
Show Summary Details
More options …

Clinical Chemistry and Laboratory Medicine (CCLM)

Published in Association with the European Federation of Clinical Chemistry and Laboratory Medicine (EFLM)

Editor-in-Chief: Plebani, Mario

Ed. by Gillery, Philippe / Greaves, Ronda / Lackner, Karl J. / Lippi, Giuseppe / Melichar, Bohuslav / Payne, Deborah A. / Schlattmann, Peter

IMPACT FACTOR 2018: 3.638

CiteScore 2018: 2.44

SCImago Journal Rank (SJR) 2018: 1.191
Source Normalized Impact per Paper (SNIP) 2018: 1.205

See all formats and pricing
More options …
Volume 52, Issue 12


UF-1000i: validation of the body fluid mode for counting cells in body fluids

Chérina Fleming
  • Clinical Chemistry, Erasmus MC, University Medical Center Rotterdam, Rotterdam, The Netherlands
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Rob Brouwer
  • Clinical Chemistry, Erasmus MC, University Medical Center Rotterdam, Rotterdam, The Netherlands
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Adriaan van Alphen / Jan Lindemans
  • Clinical Chemistry, Erasmus MC, University Medical Center Rotterdam, Rotterdam, The Netherlands
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Robert de Jonge
  • Corresponding author
  • Clinical Chemistry, Erasmus MC, University Medical Center Rotterdam, Rotterdam, The Netherlands
  • Email
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
Published Online: 2014-06-25 | DOI: https://doi.org/10.1515/cclm-2014-0512


Background: We evaluated the new body fluid mode on the UF-1000i urinalysis analyzer for counting total white blood cells (WBC) and red blood cells (RBC) in continuous ambulatory peritoneal dialysis (CAPD), ascites and pleural fluids.

Methods: We collected 154 body fluid samples, and compared the results of the UF-1000i BF mode with the Fuchs-Rosenthal counting chamber and the XN-1000 BF mode. Linearity, carry over and precision were also assessed.

Results: Method comparison results showed acceptable WBC agreement between UF-1000i and chamber (y=1.27x+3.13, n=135, r=0.99) and between UF-1000i and XN (y=1.15x+0.31, n=135, r=1.00). Comparison between the UF-1000i and both comparison methods showed good agreement for RBC counts. Overall results were better when UF-1000i was compared with the XN-1000 than with the Fuchs-Rosenthal chamber. The lower limit of quantitation was defined at 9×106 WBC/L and at 25×106 RBC/L. Linearity for both WBC (r=1.00) and RBC (r=0.99) was good. Carry over was negligible, and it never exceeded 0.01%. In one sample, a high discrepancy was observed between WBC results for both automated analyzers and the counting chamber. This discrepancy was due to interfering factors, such as bacteria and yeast cells, and it led to a false increased WBC count on both automated systems.

Conclusions: The UF-1000i BF mode offers rapid and reliable total WBC and RBC counts for initial screening of CAPD, ascites and pleural fluid, and can improve the workflow in a routine laboratory; however, when using automated analyzers, the inspection of scattergrams is required to ensure the most accurate results are obtained.

Keywords: body fluid; counting chamber; red blood cells (RBC); UF-1000i; white blood cells (WBC)


  • 1.

    de Jonge R, Brouwer R, de Graaf MT, Luitwieler RL, Fleming C, de Frankrijker-Merkestijn M, et al. Evaluation of the new body fluid mode on the Sysmex XE-5000 for counting leukocytes and erythrocytes in cerebrospinal fluid and other body fluids. Clin Chem Lab Med 2010;48:665–75.Web of ScienceGoogle Scholar

  • 2.

    Barnes PW, Eby CS, Shimer G. An evaluation of the utility of performing body fluid counts on the Coulter LH 750. Lab Hematol 2004;10:127–31.Google Scholar

  • 3.

    de Jonge R, Brouwer R, van Rijn M, van Acker BA, Otten HJ, Lindemans J. Automated analysis of pleural fluid total and differential leukocyte counts with the Sysmex XE-2100. Clin Chem Lab Med 2006;44:1367–71.Google Scholar

  • 4.

    De Smet D, Van Moer G, Martens GA, Nanos N, Smet L, Jochmans K, et al. Use of the Cell-Dyn Sapphire hematology analyzer for automated counting of blood cells in body fluids. Am J Clin Path 2010;133:291–9.Google Scholar

  • 5.

    Angeloni S, Nicolini G, Merli M, Nicolao F, Pinto G, Aronne T, et al. Validation of automated blood cell counter for the determination of polymorphonuclear cell count in the ascitic fluid of cirrhotic patients with or without spontaneous bacterial peritonitis. Am J Gastroenterol 2003;98:1844–8.CrossrefPubMedGoogle Scholar

  • 6.

    Zimmermann M, Ruprecht K, Kainzinger F, Heppner FL, Weimann A. Automated vs. manual cerebrospinal fluid cell counts: a work and cost analysis comparing the Sysmex XE-5000 and the Fuchs-Rosenthal manual counting chamber. Int J Lab Hematol 2011;33:629–37.Web of ScienceGoogle Scholar

  • 7.

    Fleming Chérina RB, Lindemans J, deJonge R. Validation of the body fluid module on the new Sysmex XN-1000 for counting blood cells in cerebrospinal fluid and other body fluids. Clin Chem Lab Med 2012;50:1791–8.Web of ScienceGoogle Scholar

  • 8.

    Butch AW, Wises PK, Wah DT, Gornet TG, Fritsche HA. A multicenter evaluation of the Iris iQ200 automated urine microscopy analyzer body fluids module and comparison with hemacytometer cell counts. Am J Clin Pathol 2008;129:445–50.Google Scholar

  • 9.

    Boer K, Deufel T, Reinhoefer M. Evaluation of the XE-5000 for the automated analysis of blood cells in cerebrospinal fluid. Clin Biochem 2009;42:684–91.Web of ScienceGoogle Scholar

  • 10.

    Perné A, Hainfellner JA, Womastek I, Haushofer A, Szekeres T, Schwarzinger I. Performance evaluation of the Sysmex XE-5000 hematology analyzer for white blood cell analysis in cerebrospinal fluid. Arch Pathol Lab Med 2012;136:194–8.Web of ScienceGoogle Scholar

  • 11.

    Van Acker JT, Delanghe JR, Langlois MR, Taes YE, De Buyzere ML, Verstraete AG. Automated flow cytometric analysis of cerebrospinal fluid. Clin Chem 2001;47:556–60.PubMedGoogle Scholar

  • 12.

    Yamanishi H, Imai N, Ohmine T, Nishiyama M, Suehisa E, Kanakura Y, et al. Urine flow cytometer quantification of leukocytes in samples containing a large proportion of lymphocytes. Clin Biochem 2006;39:857–9.PubMedCrossrefGoogle Scholar

  • 13.

    Penders J, Fiers T, Dhondt AM, Claeys G, Delanghe JR. Automated flow cytometry analysis of peritoneal dialysis fluid. Nephrol Dial Transpl 2004;19:463–8.CrossrefGoogle Scholar

  • 14.

    Ziebig R, Lun A, Sinha P. Leukocyte counts in cerebrospinal fluid with the automated hematology analyzer CellDyn 3500 and the urine flow cytometer UF-100. Clin Chem 2000;46:242–7.Google Scholar

  • 15.

    Nanos NE, Delanghe JR. Evaluation of Sysmex UF-1000i for use in cerebrospinal fluid analysis. Clin Chim Acta 2008;392: 30–3.Web of ScienceGoogle Scholar

  • 16.

    England JM, Rowan RM, Assendelft OW, Bull BS, Coulter WH, McLaren CE. Guidelines for the evaluation of blood cell analysers including those used for differential leucocyte and reticulocyte counting and cell marker applications. Clin Lab Haematol 1994;16:157–74.Google Scholar

  • 17.

    Keane WF, Bailie GR, Boeschoten E, Gokal R, Golper TA, Holmes CJ, et al. Adult peritoneal dialysis-related peritonitis treatment recommendations: 2000 update. Perit Dial Int 2000;20: 396–411.PubMedGoogle Scholar

  • 18.

    Rimola A, Garcia-Tsao G, Navasa M, Piddock LJ, Planas R, Bernard B, et al. Diagnosis, treatment and prophylaxis of spontaneous bacterial peritonitis: a consensus document. International Ascites Club. J Hepatol 2000;32:142–53.CrossrefPubMedGoogle Scholar

  • 19.

    Soogarun S, Sirimongkolsakul S, Wiwanitkit V, Siritantikorn A, Pimsane K, Srivijarn R. Leukocyte counts in cerebrospinal fluid with the automated hematology analyzer, Technicon H*3. Clin Lab 2002;48:623–9.Google Scholar

  • 20.

    Omuse G, Makau P. Interference of cerebrospinal fluid white blood cell counts performed on the Sysmex XT-4000i by yeast and bacteria. Int J Lab Hematol 2013;35:5–7.Web of ScienceGoogle Scholar

  • 21.

    Langlois MR, Delanghe JR, Steyaert SR, Everaert KC, De Buyzere ML. Automated flow cytometry compared with an automated dipstick reader for urinalysis. Clin Chem 1999;45:118–22.PubMedGoogle Scholar

  • 22.

    Conner BD, Lee YC, Branca P, Rogers JT, Rodriguez RM, Light RW. Variations in pleural fluid wbc count and differential counts with different sample containers and different methods. Chest 2003;123:1181–7.CrossrefPubMedGoogle Scholar

About the article

Corresponding author: Dr. Robert de Jonge, Department of Clinical Chemistry, Na-412 Erasmus MC, University Medical Center Rotterdam, Wytemaweg 80, 3015 CN Rotterdam, The Netherlands, Phone: +31 107033473, Fax: +31 107044895, E-mail:

Received: 2014-05-12

Accepted: 2014-06-02

Published Online: 2014-06-25

Published in Print: 2014-12-01

Citation Information: Clinical Chemistry and Laboratory Medicine (CCLM), Volume 52, Issue 12, Pages 1781–1790, ISSN (Online) 1437-4331, ISSN (Print) 1434-6621, DOI: https://doi.org/10.1515/cclm-2014-0512.

Export Citation

©2014 by De Gruyter.Get Permission

Citing Articles

Here you can find all Crossref-listed publications in which this article is cited. If you would like to receive automatic email messages as soon as this article is cited in other publications, simply activate the “Citation Alert” on the top of this page.

A. Maleb, O. Bouayadi, J. El Malki, S. Rifai, S. Lamrabat, E. Benaissa, Y. Ben Lahlou, M. Frikh, and M. Elouennass
Analytical Biochemistry, 2019, Page 113501
Jooyoung Cho, Joowon Oh, Sang-Guk Lee, You-Hee Lee, Jaewoo Song, and Jeong-Ho Kim
Annals of Laboratory Medicine, 2020, Volume 40, Number 2, Page 122
Mo Sae Koo, Jinsook Lim, Seon Young Kim, Sun Hoe Koo, and Gye Cheol Kwon
Journal of Laboratory Medicine and Quality Assurance, 2019, Volume 41, Number 3, Page 172
Yimin Shen, Jun Cao, Zhiying Zhou, Yong Wang, Yili Shen, and Jun He
International Journal of Laboratory Hematology, 2019, Volume 41, Number 5, Page 622
Sabrina Buoro, Michela Seghezzi, Maria del Carmen Baigorria Vaca, Barbara Manenti, Valentina Moioli, Giulia Previtali, Caterina Simon, Diego Cugola, Antonio Brucato, Cosimo Ottomano, and Giuseppe Lippi
Journal of Clinical Pathology, 2019, Volume 72, Number 7, Page 493
Paolo Angeli, Mauro Bernardi, Càndid Villanueva, Claire Francoz, Rajeshwar P. Mookerjee, Jonel Trebicka, Aleksander Krag, Wim Laleman, and Pere Gines
Journal of Hepatology, 2018
María I. Millán-Lou, Juan M. García-Lechuz, María A. Ruiz-Andrés, Concepción López, María J. Aldea, María J. Revillo, and Antonio Rezusta
Frontiers in Medicine, 2018, Volume 5
A. Larruzea, V. Aguadero, R. Orellana, and E. Berlanga
International Journal of Laboratory Hematology, 2018
Vicente Aguadero, Ruth Cano-Corres, Eugenio Berlanga, and Montserrat Torra
Cytometry Part B: Clinical Cytometry, 2017
Weiyi Xu, Qian Yu, Lixia Xie, Baode Chen, and Ling Zhang
Medicine, 2017, Volume 96, Number 27, Page e7433
S. Buoro, T. Mecca, G. Azzarà, M. Seghezzi, E. Candiago, A. Gianatti, A. Crippa, and A. La Gioia
International Journal of Laboratory Hematology, 2016, Volume 38, Number 1, Page 90
Sabrina Buoro, Sara Apassiti Esposito, MariaGrazia Alessio, Alberto Crippa, Cosimo Ottomano, and Giuseppe Lippi
Journal of Clinical Laboratory Analysis, 2016, Volume 30, Number 5, Page 381
D. Labaere, N. Boeckx, I. Geerts, M. Moens, and M. Van den Driessche
International Journal of Laboratory Hematology, 2015, Volume 37, Number 5, Page 715

Comments (0)

Please log in or register to comment.
Log in