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Clinical Chemistry and Laboratory Medicine (CCLM)

Published in Association with the European Federation of Clinical Chemistry and Laboratory Medicine (EFLM)

Editor-in-Chief: Plebani, Mario

Ed. by Gillery, Philippe / Greaves, Ronda / Lackner, Karl J. / Lippi, Giuseppe / Melichar, Bohuslav / Payne, Deborah A. / Schlattmann, Peter


IMPACT FACTOR 2018: 3.638

CiteScore 2018: 2.44

SCImago Journal Rank (SJR) 2018: 1.191
Source Normalized Impact per Paper (SNIP) 2018: 1.205

Online
ISSN
1437-4331
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Volume 52, Issue 2

Issues

Digital immunofluorescence enables automated detection of antinuclear antibody endpoint titers avoiding serial dilution

Dirk Roggenbuck
  • Corresponding author
  • Faculty of Natural Sciences, Brandenburg Technical University Cottbus-Senftenberg, Senftenberg, Germany
  • GA Generic Assays GmbH, Dahlewitz/Berlin, Germany
  • Email
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Rico Hiemann
  • Faculty of Natural Sciences, Brandenburg Technical University Cottbus-Senftenberg, Senftenberg, Germany
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Peter Schierack
  • Faculty of Natural Sciences, Brandenburg Technical University Cottbus-Senftenberg, Senftenberg, Germany
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Dirk Reinhold
  • Institute of Molecular and Clinical Immunology, Otto-von-Guericke-University, Magdeburg, Germany
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Karsten Conrad
Published Online: 2013-08-27 | DOI: https://doi.org/10.1515/cclm-2013-0543

References

  • 1.

    Bonroy C, Verfaillie C, Smith V, Persijn L, De WE, De KF, et al. Automated indirect immunofluorescence antinuclear antibody analysis is a standardized alternative for visual microscope interpretation. Clin Chem Lab Med 2013;51:1771–9.CrossrefPubMedWeb of ScienceGoogle Scholar

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    Willitzki A, Hiemann R, Peters V, Sack U, Schierack P, Rödiger S, et al. New platform technology for comprehensive serological diagnostics of autoimmune diseases. Clin Develop Immunol 2012;2012:284740.Web of ScienceGoogle Scholar

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    Roggenbuck D, Hiemann R, Bogdanos D, Reinhold D, Conrad K. Standardization of automated interpretation of immunofluorescence tests. Clin Chim Acta 2013;421C:168–9.Web of ScienceGoogle Scholar

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    Hiemann R, Buttner T, Krieger T, Roggenbuck D, Sack U, Conrad K. Challenges of automated screening and differentiation of non-organ specific autoantibodies on HEp-2 cells. Autoimmun Rev 2009;9:17–22.CrossrefPubMedWeb of ScienceGoogle Scholar

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    Tozzoli R, Bonaguri C, Melegari A, Antico A, Bassetti D, Bizzaro N. Current state of diagnostic technologies in the autoimmunology laboratory. Clin Chem Lab Med 2012;51:1–10.Web of ScienceGoogle Scholar

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    Knutter I, Hiemann R, Brumma T, Buttner T, Grossmann K, Cusini M, et al. Automated interpretation of ANCA patterns – a new approach in the serology of ANCA-associated vasculitis. Arthritis Res Ther 2012;14:R271.CrossrefGoogle Scholar

  • 7.

    Roggenbuck D, Reinhold D, Hiemann R, Anderer U, Conrad K. Standardized detection of anti-ds DNA antibodies by indirect immunofluorescence – A new age for confirmatory tests in SLE diagnostics. Clin Chim Acta 2011;412:2011–2.Web of ScienceGoogle Scholar

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    Melegari A, Bonaguri C, Russo A, Luisita B, Trenti T, Lippi G. A comparative study on the reliability of an automated system for the evaluation of cell-based indirect immunofluorescence. Autoimmun Rev 2012;11:713–6.CrossrefWeb of SciencePubMedGoogle Scholar

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    Sack U, Conrad K, Csernok E, Frank I, Hiepe F, Krieger T, et al. Autoantibody detection using indirect immunofluorescence on HEp-2 cells. Ann N Y Acad Sci 2009;1173:166–73.CrossrefGoogle Scholar

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    Van Blerk M, Van Campenhout C, Bossuyt X, Duchateau J, Humbel R, Servais G, et al. Current practices in antinuclear antibody testing: results from the Belgian External Quality Assessment Scheme. Clin Chem Lab Med 2009;47:102–8.PubMedWeb of ScienceCrossrefGoogle Scholar

About the article

Corresponding author: Prof. Dr. Dirk Roggenbuck, MD, PhD, Faculty of Natural Sciences, Brandenburg Technical University Cottbus-Senftenberg, Großenhainer Strasse 57, 01968 Senftenberg, Germany, Phone: +49 33708 441716, Fax: +49 33708 441725, E-mail: ; and GA Generic Assays GmbH, Dahlewitz/Berlin, Germany


Received: 2013-07-18

Accepted: 2013-08-07

Published Online: 2013-08-27

Published in Print: 2014-02-01


Citation Information: Clinical Chemistry and Laboratory Medicine (CCLM), Volume 52, Issue 2, Pages e9–e11, ISSN (Online) 1437-4331, ISSN (Print) 1434-6621, DOI: https://doi.org/10.1515/cclm-2013-0543.

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