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Clinical Chemistry and Laboratory Medicine (CCLM)

Published in Association with the European Federation of Clinical Chemistry and Laboratory Medicine (EFLM)

Editor-in-Chief: Plebani, Mario

Ed. by Gillery, Philippe / Lackner, Karl J. / Lippi, Giuseppe / Melichar, Bohuslav / Payne, Deborah A. / Schlattmann, Peter / Tate, Jillian R.

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Rank 5 out of 30 in category Medical Laboratory Technology in the 2014 Thomson Reuters Journal Citation Report/Science Edition

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A soluble form of the macrophage-related mannose receptor (MR/CD206) is present in human serum and elevated in critical illness

Sidsel Rødgaard-Hansen1 / Aisha Rafique1 / Peter A. Christensen1 / Maciej B. Maniecki1 / Thomas D. Sandahl2 / Ebba Nexø1 / 1

1Department of Clinical Biochemistry, Aarhus University Hospital, Aarhus, Denmark

2Department of Medicine V, Aarhus University Hospital, Aarhus, Denmark

Corresponding author: Dr. Holger Jon Møller, Department of Clinical Biochemistry, Aarhus University Hospital, Nørrebrogade 44, 8000 Aarhus C, Denmark, Phone: +45 3120 2201, Fax: +45 7846 3060, E-mail:

Citation Information: Clinical Chemistry and Laboratory Medicine. Volume 52, Issue 3, Pages 453–461, ISSN (Online) 1437-4331, ISSN (Print) 1434-6621, DOI: 10.1515/cclm-2013-0451, October 2013

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This article offers supplementary material which is provided at the end of the article.


Background: This study tests the hypothesis that the mannose receptor (MR/CD206), which is expressed primarily by macrophages and dendritic cells, can be found in a soluble form (sMR, sMR) in human serum. Furthermore, we wished to establish and validate an enzyme-linked immunosorbent assay (ELISA) for sMR and to perform initial studies exploring the potential of sMR as a biomarker.

Methods: Western blotting identified a single band of approximately 170 kDa in human serum, and MALDI MS/MS of the purified protein confirmed it to be sMR. An ELISA was established and validated with a measurement range of 1–256 µg/L.

Results: The 95% reference interval was 0.10–0.43 mg/L based on measurements of serum samples from healthy individuals (n=217). Samples from hospitalised patients (n=219) revealed that more than 50% of patients had concentrations above 0.43 mg/L. Very high concentrations (up to 6.2 mg/L) were observed in critically ill patients with sepsis and/or severe liver disease.

Conclusions: This study documents, for the first time, the presence of sMR in human serum and describes an optimised ELISA suitable for quantitative measurements. Levels of sMR are strongly elevated in several disease states, including sepsis and liver disease, and the protein therefore shows promise as a new biomarker.

Keywords: biomarker; CD206; hepatitis; liver disease; M2 macrophage; sCD206; sepsis; sMR; soluble mannose receptor

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Henning Grønbæk, Sidsel Rødgaard-Hansen, Niels Kristian Aagaard, Vicente Arroyo, Søren K. Moestrup, Elisabet Garcia, Elsa Solà, Marco Domenicali, Salvatore Piano, Hendrik Vilstrup, and Holger Jon Møller
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Morten N. Andersen, Niels F. Andersen, Sidsel Rødgaard-Hansen, Marianne Hokland, Niels Abildgaard, and Holger J. Møller
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Frontiers in Immunology, 2014, Volume 5

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