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Clinical Chemistry and Laboratory Medicine (CCLM)

Published in Association with the European Federation of Clinical Chemistry and Laboratory Medicine (EFLM)

Editor-in-Chief: Plebani, Mario

Ed. by Gillery, Philippe / Lackner, Karl J. / Lippi, Giuseppe / Melichar, Bohuslav / Payne, Deborah A. / Schlattmann, Peter / Tate, Jillian R.

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Volume 52, Issue 3 (Mar 2014)


A soluble form of the macrophage-related mannose receptor (MR/CD206) is present in human serum and elevated in critical illness

Sidsel Rødgaard-Hansen / Aisha Rafique / Peter A. Christensen / Maciej B. Maniecki / Thomas D. Sandahl / Ebba Nexø / Holger Jon Møller
Published Online: 2013-10-11 | DOI: https://doi.org/10.1515/cclm-2013-0451


Background: This study tests the hypothesis that the mannose receptor (MR/CD206), which is expressed primarily by macrophages and dendritic cells, can be found in a soluble form (sMR, sMR) in human serum. Furthermore, we wished to establish and validate an enzyme-linked immunosorbent assay (ELISA) for sMR and to perform initial studies exploring the potential of sMR as a biomarker.

Methods: Western blotting identified a single band of approximately 170 kDa in human serum, and MALDI MS/MS of the purified protein confirmed it to be sMR. An ELISA was established and validated with a measurement range of 1–256 µg/L.

Results: The 95% reference interval was 0.10–0.43 mg/L based on measurements of serum samples from healthy individuals (n=217). Samples from hospitalised patients (n=219) revealed that more than 50% of patients had concentrations above 0.43 mg/L. Very high concentrations (up to 6.2 mg/L) were observed in critically ill patients with sepsis and/or severe liver disease.

Conclusions: This study documents, for the first time, the presence of sMR in human serum and describes an optimised ELISA suitable for quantitative measurements. Levels of sMR are strongly elevated in several disease states, including sepsis and liver disease, and the protein therefore shows promise as a new biomarker.

This article offers supplementary material which is provided at the end of the article.

Keywords: biomarker; CD206; hepatitis; liver disease; M2 macrophage; sCD206; sepsis; sMR; soluble mannose receptor


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About the article

Corresponding author: Dr. Holger Jon Møller, Department of Clinical Biochemistry, Aarhus University Hospital, Nørrebrogade 44, 8000 Aarhus C, Denmark, Phone: +45 3120 2201, Fax: +45 7846 3060, E-mail:

Received: 2013-06-14

Accepted: 2013-09-04

Published Online: 2013-10-11

Published in Print: 2014-03-01

Citation Information: Clinical Chemistry and Laboratory Medicine, ISSN (Online) 1437-4331, ISSN (Print) 1434-6621, DOI: https://doi.org/10.1515/cclm-2013-0451.

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