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Clinical Chemistry and Laboratory Medicine (CCLM)

Published in Association with the European Federation of Clinical Chemistry and Laboratory Medicine (EFLM)

Editor-in-Chief: Plebani, Mario

Ed. by Gillery, Philippe / Greaves, Ronda / Lackner, Karl J. / Lippi, Giuseppe / Melichar, Bohuslav / Payne, Deborah A. / Schlattmann, Peter


IMPACT FACTOR 2018: 3.638

CiteScore 2018: 2.44

SCImago Journal Rank (SJR) 2018: 1.191
Source Normalized Impact per Paper (SNIP) 2018: 1.205

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1437-4331
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Volume 53, Issue 1

Issues

The 13C-glucose breath test is a valid non-invasive screening tool to identify metabolic syndrome in adolescents

Alejandra Salas-Fernández
  • Mass Spectrometry Laboratory, Research Unit in Medical Nutrition, Pediatric Hospital, National Medical Center 21st Century, Mexican Institute of Social Security, Mexico City, Mexico
  • Other articles by this author:
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/ Jorge Maldonado-Hernández
  • Corresponding author
  • Mass Spectrometry Laboratory, Research Unit in Medical Nutrition, Pediatric Hospital, National Medical Center 21st Century, Mexican Institute of Social Security, Mexico City, Mexico
  • Email
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Azucena Martínez-Basila
  • Mass Spectrometry Laboratory, Research Unit in Medical Nutrition, Pediatric Hospital, National Medical Center 21st Century, Mexican Institute of Social Security, Mexico City, Mexico
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Gabriel Martínez-Razo
  • Mass Spectrometry Laboratory, Research Unit in Medical Nutrition, Pediatric Hospital, National Medical Center 21st Century, Mexican Institute of Social Security, Mexico City, Mexico
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Filiberto Jasso-Saavedra
  • Mass Spectrometry Laboratory, Research Unit in Medical Nutrition, Pediatric Hospital, National Medical Center 21st Century, Mexican Institute of Social Security, Mexico City, Mexico
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
Published Online: 2014-08-07 | DOI: https://doi.org/10.1515/cclm-2014-0412

Abstract

Background: Metabolic syndrome (MS) is an important risk factor in pediatric population for the early onset of type 2 diabetes mellitus and cardiovascular disease. New non-invasive tools are required to identify MS in at risk populations; the aim of this study was to determine an optimal cut-off point for the 13C-glucose breath test (13C-GBT) for the diagnosis of MS in adolescents.

Methods: A total of 136 adolescents between 10 and 16 years old were recruited. MS was defined as: waist circumference >90th percentile and at least two of the following; high density lipoprotein-cholesterol (HDL-C) <50 mg/dL, triglycerides >110 mg/dL, diastolic and/or systolic blood pressure >90th percentile adjusted by age, gender and height, and/or fasting glucose >100 mg/dL. After the ingestion of a glucose load of 1.75 g/kg of body weight (up to 75 g) and an oral dose of 1.5 mg of universally labeled 13C-glucose/kg dissolved in water, breath samples were taken at baseline, 30, 60, 90, 120, 150 and 180 min. Exhaled 13CO2 in breath samples was measured by isotope ratio mass spectrometry.

Results: 13C-GBT data, expressed as adjusted cumulative percentage of oxidized dose (A% OD) at 180 min, was significantly higher in the healthy subjects group (17.72%±4.9%) in comparison with subjects with ≥3, 2 or 1 components of the MS (9.95%±4.73%, 14.3%±4.47% and 14.62%±4.62%, respectively). The optimal cut-off point for the A% OD was 16.09, with a sensitivity of 81.5% and a specificity of 66.7%.

Conclusions: Our results demonstrate that the 13C-glucose breath test could be a valid screening method to identify MS in adolescents.

Keywords: adolescents; 13C-glucose breath test; metabolic syndrome; validity

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About the article

Corresponding author: Jorge Maldonado-Hernández, Mass Spectrometry Laboratory, Research Unit in Medical Nutrition, Pediatric Hospital, National Medical Center 21st Century, Mexican Institute of Social Security, Mexico City, Av. Cuauhtémoc No. 330, Col. Doctores, 06720 Mexico City, Mexico, Phone/Fax: +52 55 56276944, E-mail:


Received: 2014-04-14

Accepted: 2014-06-25

Published Online: 2014-08-07

Published in Print: 2015-01-01


Citation Information: Clinical Chemistry and Laboratory Medicine (CCLM), Volume 53, Issue 1, Pages 133–138, ISSN (Online) 1437-4331, ISSN (Print) 1434-6621, DOI: https://doi.org/10.1515/cclm-2014-0412.

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