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Clinical Chemistry and Laboratory Medicine (CCLM)

Published in Association with the European Federation of Clinical Chemistry and Laboratory Medicine (EFLM)

Editor-in-Chief: Plebani, Mario

Ed. by Gillery, Philippe / Greaves, Ronda / Lackner, Karl J. / Lippi, Giuseppe / Melichar, Bohuslav / Payne, Deborah A. / Schlattmann, Peter


IMPACT FACTOR 2018: 3.638

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Volume 53, Issue 10

Issues

First trimester concentrations of the TTR-RBP4-retinol complex components as early markers of insulin-treated gestational diabetes mellitus

Arrigo Fruscalzo
  • Corresponding author
  • Clinic of Obstetrics and Gynecology, St. Franziskus-Hospital, Münster, Germany
  • Clinic of Obstetrics and Gynecology, University of Münster, Münster, Germany
  • Email
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Ambrogio P. Londero
  • Clinic of Obstetrics and Gynecology, DISM, AOU “SM della Misericordia” of Udine, Udine, Italy
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Lorenza Driul
  • Clinic of Obstetrics and Gynecology, DISM, AOU “SM della Misericordia” of Udine, Udine, Italy
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Andrea Henze / Laura Tonutti
  • Department for Endocrinology and Metabolism, AOU “SM della Misericordia” of Udine, Udine, Italy
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  • De Gruyter OnlineGoogle Scholar
/ Maria Ceraudo
  • Institute of Clinical Pathology, Department of Laboratory Medicine, University of Udine, Udine, Italy
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Giuseppe Zanotti / Rodolfo Berni / Florian J. Schweigert / Jens Raila
Published Online: 2015-03-14 | DOI: https://doi.org/10.1515/cclm-2014-0929

Abstract

Background: The objective of the study was to investigate the relationship between first trimester maternal serum levels of the TTR-RBP4-ROH complex components and the later insurgence of an altered glucose metabolism during pregnancy.

Methods: Retrospective case control study including 96 patients between the 12th and 14th week of gestation, 32 that developed gestational diabetes mellitus (GDM), respectively, 21 non-insulin-treated (dGDM) and 11 insulin-treated (iGDM), 20 large for gestational age fetuses (LGA) without GDM and 44 patients with normal outcome as control. Serum concentrations of RBP4 and TTR were assessed by ELISA; serum concentration of ROH by reverse-phase high performance liquid chromatography (rpHPLC). The molecular heterogeneity of TTR and RBP4 was analyzed after immunoprecipitation by matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF-MS).

Results: iGDM patients were characterized by reduced TTR, RBP4 and ROH compared to controls (respectively, iGDM vs. controls, mean±SD: TTR 3.96±0.89 μmol/L vs. 4.68±1.21 μmol/L, RBP4 1.13±0.25 μmol/L vs. 1.33±0.38 μmol/L and ROH 1.33±0.17 μmol/L vs. 1.62±0.29 μmol/L, p<0.05). TTR containing Gly10 in place of Cys10 was lower in the iGDM group (p<0.05) compared to controls. In the final logistic regression model ROH significantly predicted the diagnosis of iGDM (OR 0.93, 95% CI 0.87–0.98, p<0.05).

Conclusions: First trimester maternal serum ROH, RBP4 and TTR represent potential biomarkers associated with the development of iGDM.

Keywords: first trimester; gestational diabetes mellitus (GDM); insulin; large for gestational age fetus (LGA); protein microheterogeneity; retinol (ROH); serum retinol binding protein (RBP4); transthyretin (TTR)

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About the article

Corresponding author: Arrigo Fruscalzo, St. Franziskus-Hospital, Hohenzollernring 72, 48145 Münster, Germany, Phone: +49 02 51 9353917, Fax: +49 02 51 9354072, E-mail:


Received: 2014-09-19

Accepted: 2015-02-10

Published Online: 2015-03-14

Published in Print: 2015-09-01


Citation Information: Clinical Chemistry and Laboratory Medicine (CCLM), Volume 53, Issue 10, Pages 1643–1651, ISSN (Online) 1437-4331, ISSN (Print) 1434-6621, DOI: https://doi.org/10.1515/cclm-2014-0929.

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