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Clinical Chemistry and Laboratory Medicine (CCLM)

Published in Association with the European Federation of Clinical Chemistry and Laboratory Medicine (EFLM)

Editor-in-Chief: Plebani, Mario

Ed. by Gillery, Philippe / Greaves, Ronda / Lackner, Karl J. / Lippi, Giuseppe / Melichar, Bohuslav / Payne, Deborah A. / Schlattmann, Peter


IMPACT FACTOR 2018: 3.638

CiteScore 2018: 2.44

SCImago Journal Rank (SJR) 2018: 1.191
Source Normalized Impact per Paper (SNIP) 2018: 1.205

Online
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1437-4331
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Volume 53, Issue 12

Issues

Diagnostic performances of clinical laboratory tests using Triton X-100 to reduce the biohazard associated with routine testing of Ebola virus-infected patients

Massimo Tempestilli
  • Clinical Biochemistry and Pharmacology Laboratory, National Institute for Infectious Diseases, “L. Spallanzani” IRCCS, Rome, Italy
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/ Luigia Pucci
  • Clinical Biochemistry and Pharmacology Laboratory, National Institute for Infectious Diseases, “L. Spallanzani” IRCCS, Rome, Italy
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/ Stefania Notari
  • Clinical Biochemistry and Pharmacology Laboratory, National Institute for Infectious Diseases, “L. Spallanzani” IRCCS, Rome, Italy
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/ Antonino Di Caro
  • Laboratory of Microbiology and Infectious Disease Biorepository, National Institute for Infectious Diseases, “L. Spallanzani” IRCCS, Rome, Italy
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/ Concetta Castilletti
  • Laboratory of Virology, National Institute for Infectious Diseases, “L. Spallanzani” IRCCS, Rome, Italy
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/ Maria Rosaria Rivelli / Chiara Agrati
  • Laboratory of Virology, National Institute for Infectious Diseases, “L. Spallanzani” IRCCS, Rome, Italy
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/ Leopoldo Paolo Pucillo
  • Corresponding author
  • Clinical Biochemistry and Pharmacology Laboratory, National Institute for Infectious Diseases, “L. Spallanzani” IRCCS, Rome, Italy
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Published Online: 2015-06-06 | DOI: https://doi.org/10.1515/cclm-2015-0119

Abstract

Background: Ebola virus, an enveloped virus, is the cause of the largest and most complex Ebola virus disease (EVD) outbreak in West Africa. Blood or body fluids of an infected person may represent a biohazard to laboratory workers. Laboratory tests of virus containing specimens should be conducted in referral centres at biosafety level 4, but based on the severity of clinical symptoms, basic laboratories might be required to execute urgent tests for patients suspected of EVD. The aim of this work was to compare the analytical performances of laboratory tests when Triton X-100, a chemical agent able to inactivate other enveloped viruses, was added to specimens.

Methods: Results of clinical chemistry, coagulation and haematology parameters on samples before and after the addition of 0.1% (final concentration) of Triton X-100 and 1 h of incubation at room temperature were compared.

Results: Overall, results showed very good agreement by all statistical analyses. Triton X-100 at 0.1% did not significantly affect the results for the majority of the analytes tested.

Conclusions: Triton X-100 at 0.1% can be used to reduce the biohazard in performing laboratory tests on samples from patients with EVD without affecting clinical decisions.

This article offers supplementary material which is provided at the end of the article.

Keywords: clinical chemistry; coagulation; Ebola; haematology; Triton

References

About the article

Corresponding author: Leopoldo Paolo Pucillo, Clinical Biochemistry and Pharmacology Laboratory, “L.Spallanzani” National Institute for Infectious Diseases IRCCS, Via Portuense 292, 00149 Rome, Italy, Phone: +39 06 55170250, Fax: +39 06 5599340, E-mail:


Received: 2015-02-03

Accepted: 2015-05-07

Published Online: 2015-06-06

Published in Print: 2015-11-01


Citation Information: Clinical Chemistry and Laboratory Medicine (CCLM), Volume 53, Issue 12, Pages 1967–1973, ISSN (Online) 1437-4331, ISSN (Print) 1434-6621, DOI: https://doi.org/10.1515/cclm-2015-0119.

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