Jump to ContentJump to Main Navigation
Show Summary Details
More options …

Clinical Chemistry and Laboratory Medicine (CCLM)

Published in Association with the European Federation of Clinical Chemistry and Laboratory Medicine (EFLM)

Editor-in-Chief: Plebani, Mario

Ed. by Gillery, Philippe / Greaves, Ronda / Lackner, Karl J. / Lippi, Giuseppe / Melichar, Bohuslav / Payne, Deborah A. / Schlattmann, Peter


IMPACT FACTOR 2018: 3.638

CiteScore 2018: 2.44

SCImago Journal Rank (SJR) 2018: 1.191
Source Normalized Impact per Paper (SNIP) 2018: 1.205

Online
ISSN
1437-4331
See all formats and pricing
More options …
Volume 53, Issue 9

Issues

Influence of ABO type on global coagulation assay results: effect of coagulation factor VIII

Qute Choi
  • Department of Laboratory Medicine, Seoul National University College of Medicine, Seoul, Republic of Korea
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Ji-Eun Kim
  • Department of Laboratory Medicine, Seoul National University College of Medicine, Seoul, Republic of Korea
  • Cancer Research Institute, Seoul National University College of Medicine, Seoul, Republic of Korea
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Seon Young Kim
  • Department of Laboratory Medicine, Seoul National University College of Medicine, Seoul, Republic of Korea
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Kyou Sup Han
  • Department of Laboratory Medicine, Seoul National University College of Medicine, Seoul, Republic of Korea
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Hyun Kyung Kim
  • Corresponding author
  • Department of Laboratory Medicine, Seoul National University College of Medicine, Seoul, Republic of Korea
  • Cancer Research Institute, Seoul National University College of Medicine, Seoul, Republic of Korea
  • Email
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
Published Online: 2014-12-10 | DOI: https://doi.org/10.1515/cclm-2014-0909

Abstract

Background: As ABO blood type influences the plasma level of coagulation factor VIII (FVIII), it likely also affects activated partial thromboplastin time (aPTT) and thrombin generation assay (TGA) values. Here, we aimed to investigate the effect of ABO type on the normal values of three global coagulation assays: prothrombin time (PT), aPTT, and TGA.

Methods: PT, aPTT, TGA [1 or 5 pmol/L tissue factor (TF)], coagulation factors, anticoagulation factors, and ABO type were measured in 200 healthy adults.

Results: aPTT was significantly prolonged in those with type O compared with those with type non-O, whereas PT was not significantly different between those with type O and type non-O. The time to peak induced by 5 pmol/L TF was significantly prolonged, and the peak thrombin level was decreased in those with type O compared with those with type non-O. FVIII was a major contributor to the ABO-specific reference range of aPTT, 5 pmol/L TF-induced time to peak, and peak thrombin level.

Conclusions: The reference ranges of aPTT and TGA (time to peak and peak thrombin level) differed by ABO type. FVIII level is considered a major contributor to ABO type-specific differences with respect to aPTT and TGA.

Keywords: ABO type; activated partial thromboplastin time; factor VIII; normal reference value; thrombin generation assay

References

  • 1.

    Favaloro EJ, Soltani S, McDonald J, Grezchnik E, Easton L, Favaloro JW. Reassessment of ABO blood group, sex, and age on laboratory parameters used to diagnose von Willebrand disorder – Potential influence on the diagnosis vs the potential association with risk of thrombosis. Am J Clin Pathol 2005;124:910–7.PubMedCrossrefGoogle Scholar

  • 2.

    Gill JC, Endres-Brooks J, Bauer PJ, Marks WJ, Jr., Montgomery RR. The effect of ABO blood group on the diagnosis of von Willebrand disease. Blood 1987;69:1691–5.PubMedGoogle Scholar

  • 3.

    Jenkins PV, O’Donnell JS. ABO blood group determines plasma von Willebrand factor levels: a biologic function after all? Transfusion 2006;46:1836–44.CrossrefGoogle Scholar

  • 4.

    O’Donnell J, Laffan MA. The relationship between ABO histo-blood group, factor VIII and von Willebrand factor. Transfus Med 2001;11:343–51.CrossrefGoogle Scholar

  • 5.

    Preston AE, Barr A. The plasma concentration of factor VIII in the normal population. II. The effects of age, sex and blood group. Br J Haematol 1964;10:238–45.Google Scholar

  • 6.

    Favaloro EJ, Soltani S, McDonald J, Grezchnik E, Easton L. Cross-laboratory audit of normal reference ranges and assessment of ABO blood group, gender and age on detected levels of plasma coagulation factors. Blood Coagul Fibrinolysis 2005;16:597–605.CrossrefPubMedGoogle Scholar

  • 7.

    Qi L, Cornelis MC, Kraft P, Jensen M, van Dam RM, Sun Q, et al. Genetic variants in ABO blood group region, plasma soluble E-selectin levels and risk of type 2 diabetes. Hum Mol Genet 2010;19:1856–62.PubMedGoogle Scholar

  • 8.

    Barbalic M, Dupuis J, Dehghan A, Bis JC, Hoogeveen RC, Schnabel RB, et al. Large-scale genomic studies reveal central role of ABO in sP-selectin and sICAM-1 levels. Hum Mol Genet 2010;19:1863–72.PubMedGoogle Scholar

  • 9.

    Lee HR, Park JS, Shin S, Roh EY, Yoon JH, Han KS, et al. Increased numbers of total nucleated and CD34+ cells in blood group O cord blood: an analysis of neonatal innate factors in the Korean population. Transfusion 2012;52:76–81.Web of ScienceCrossrefGoogle Scholar

  • 10.

    Clark P, Wu O. ABO blood groups and thrombosis: a causal association, but is there value in screening? Future Cardiol 2011;7:191–201.Google Scholar

  • 11.

    Fisanovich TI, Tolstova LG. [Relationship between the ABO blood-group system and the incidence of thrombosis of the blood vessels]. Klin Med (Mosk) 1978;56:86–9.Google Scholar

  • 12.

    Morelli VM, De Visser MC, Vos HL, Bertina RM, Rosendaal FR. ABO blood group genotypes and the risk of venous thrombosis: effect of factor V Leiden. J Thromb Haemost 2005;3:183–5.CrossrefPubMedGoogle Scholar

  • 13.

    Favaloro EJ, Soltani S, McDonald J, Grezchnik E, Easton L. Activated protein C resistance: the influence of ABO-blood group, gender and age. Thromb Res 2006;117:665–70.PubMedCrossrefGoogle Scholar

  • 14.

    Favaloro EJ, Soltani S, McDonald J, Grezchnik E, Easton L. Laboratory identification of familial thrombophilia: do the pitfalls exceed the benefits? A reassessment of ABO-blood group, gender, age, and other laboratory parameters on the potential influence on a diagnosis of protein C, protein S, and antithrombin deficiency and the potential high risk of a false positive diagnosis. Lab Hematol 2005;11:174–84.PubMedGoogle Scholar

  • 15.

    Kitchens CS. To bleed or not to bleed? Is that the question for the PTT? J Thromb Haemost 2005;3:2607–11.Web of SciencePubMedCrossrefGoogle Scholar

  • 16.

    Al Dieri R, Peyvandi F, Santagostino E, Giansily M, Mannucci PM, Schved JF, et al. The thrombogram in rare inherited coagulation disorders: its relation to clinical bleeding. Thromb Haemost 2002;88:576–82.PubMedGoogle Scholar

  • 17.

    Choi Q, Kim JE, Hyun J, Han KS, Kim HK. Contributions of procoagulants and anticoagulants to the international normalized ratio and thrombin generation assay in patients treated with warfarin: potential role of protein Z as a powerful determinant of coagulation assays. Thromb Res 2013;132:e70–5.Web of SciencePubMedCrossrefGoogle Scholar

  • 18.

    Coll E, Robles-Carrillo L, Reyes E, Francis JL, Amirkhosravi A. Assessment of protein C anticoagulant pathway by thrombin generation assay in the presence of endothelial cells. J Thromb Haemost 2013;11:1916–9.Web of SciencePubMedGoogle Scholar

  • 19.

    Seifner A, Beck G, Bayer P, Eichmeir S, Lackner F, Rogelsperger O, et al. Assessment of immunoglobulin concentrates on thrombogenic activity by thrombin generation assay, prekallikrein activator assay, and size-exclusion chromatography. Transfusion 2014;54:376–83.Web of SciencePubMedGoogle Scholar

  • 20.

    Vila V, Aznar JA, Moret A, Marco A, Navarro S, Vila C, et al. Assessment of the thrombin generation assay in haemophilia: comparative study between fresh and frozen platelet-rich plasma. Haemophilia 2013;19:318–21.PubMedWeb of ScienceCrossrefGoogle Scholar

  • 21.

    Jackson CM, Esnouf MP, Lindahl TL. A critical evaluation of the prothrombin time for monitoring oral anticoagulant therapy. Pathophysiol Haemost Thromb 2003;33:43–51.CrossrefPubMedGoogle Scholar

  • 22.

    Dargaud Y, Beguin S, Lienhart A, Al Dieri R, Trzeciak C, Bordet JC, et al. Evaluation of thrombin generating capacity in plasma from patients with haemophilia A and B. Thromb Haemost 2005;93:475–80.Google Scholar

  • 23.

    Hron G, Kollars M, Binder BR, Eichinger S, Kyrle PA. Identification of patients at low risk for recurrent venous thromboembolism by measuring thrombin generation. J Am Med Assoc 2006;296:397–402.CrossrefGoogle Scholar

  • 24.

    Zekavat OR, Haghpanah S, Dehghani J, Afrasiabi A, Peyvandi F, Karimi M. Comparison of thrombin generation assay with conventional coagulation tests in evaluation of bleeding risk in patients with rare bleeding disorders. Clin Appl Thromb Hemost 2013;20:637–44.PubMedCrossrefWeb of ScienceGoogle Scholar

  • 25.

    Kim SY, Kim JE, Kim HK, Kim I, Yoon SS, Park S. Influence of coagulation and anticoagulant factors on global coagulation assays in healthy adults. Am J Clin Pathol 2013;139:370–9.PubMedCrossrefGoogle Scholar

  • 26.

    Hemker HC, Giesen P, Al Dieri R, Regnault V, de Smedt E, Wagenvoord R, et al. Calibrated automated thrombin generation measurement in clotting plasma. Pathophysiol Haemost Thromb 2003;33:4–15.CrossrefGoogle Scholar

  • 27.

    Tang W, Schwienbacher C, Lopez LM, Ben-Shlomo Y, Oudot-Mellakh T, Johnson AD, et al. Genetic associations for activated partial thromboplastin time and prothrombin time, their gene expression profiles, and risk of coronary artery disease. Am J Hum Genet 2012;91:152–62.CrossrefPubMedWeb of ScienceGoogle Scholar

  • 28.

    Vlot AJ, Mauser-Bunschoten EP, Zarkova AG, Haan E, Kruitwagen CL, Sixma JJ, et al. The half-life of infused factor VIII is shorter in hemophiliac patients with blood group O than in those with blood group A. Thromb Haemost 2000;83:65–9.PubMedGoogle Scholar

  • 29.

    Bowen DJ. An influence of ABO blood group on the rate of proteolysis of von Willebrand factor by ADAMTS13. J Thromb Haemost 2003;1:33–40.CrossrefGoogle Scholar

  • 30.

    Tripodi A, Caldwell SH, Hoffman M, Trotter JF, Sanyal AJ. Review article: the prothrombin time test as a measure of bleeding risk and prognosis in liver disease. Aliment Pharm Therap 2007;26:141–8.CrossrefWeb of ScienceGoogle Scholar

  • 31.

    Christensen TD, Jensen C, Larsen TB, Christiansen K, Sorensen B. Thrombin generation and coagulation factor activities: evaluation and comparison with the international normalized ratio. Blood Coagul Fibrinolysis 2009;20:358–65.PubMedWeb of ScienceCrossrefGoogle Scholar

  • 32.

    Gatt A, van Veen JJ, Bowyer A, Woolley AM, Cooper P, Kitchen S, et al. Wide variation in thrombin generation in patients with atrial fibrillation and therapeutic international normalized ratio is not due to inflammation. Br J Haematol 2008;142:946–52.Google Scholar

  • 33.

    Zhao Y, Zhang J, Zhang J, Wu J. Diabetes mellitus is associated with shortened activated partial thromboplastin time and increased fibrinogen values. PLos One 2011;6:e16470.Web of ScienceCrossrefGoogle Scholar

  • 34.

    Middeldorp S. Thrombosis in women: what are the knowledge gaps in 2013? J Thromb Haemost 2013;11(Suppl 1):180–91.PubMedWeb of ScienceCrossrefGoogle Scholar

  • 35.

    Fourel V, Gabastou JM, Desroys du Roure F, Ehrhardt N, Robert A. Influence of age, sex and ABO blood group on activated partial thromboplastin time. Haemostasis 1993;23:321–6.Google Scholar

  • 36.

    Babic N, Dervisevic A, Huskic J, Music M. Coagulation factor VIII activity in diabetic patients. Med Glas 2011;8:134–9.Google Scholar

  • 37.

    Soares AL, Kazmi RS, Borges MA, Rosario PW, Fernandes AP, Sousa MO, et al. Elevated plasma factor VIII and von Willebrand factor in women with type 2 diabetes: inflammatory reaction, endothelial perturbation or else? Blood Coagul Fibrinolysis 2011;22:600–5.CrossrefPubMedWeb of ScienceGoogle Scholar

About the article

Corresponding author: Hyun Kyung Kim, MD, PhD, Department of Laboratory Medicine, Seoul National University College of Medicine, 101 Daehak-ro, Jongno-gu, Seoul 110-744, Republic of Korea, Phone: +82 2 2072 0853, Fax: +82 2 747 0359, E-mail:

aQute Choi and Ji-Eun Kim contributed equally to this work.


Received: 2014-09-14

Accepted: 2014-10-20

Published Online: 2014-12-10

Published in Print: 2015-08-01


Citation Information: Clinical Chemistry and Laboratory Medicine (CCLM), Volume 53, Issue 9, Pages 1425–1432, ISSN (Online) 1437-4331, ISSN (Print) 1434-6621, DOI: https://doi.org/10.1515/cclm-2014-0909.

Export Citation

©2015 by De Gruyter.Get Permission

Citing Articles

Here you can find all Crossref-listed publications in which this article is cited. If you would like to receive automatic email messages as soon as this article is cited in other publications, simply activate the “Citation Alert” on the top of this page.

[1]
Catherine Ravanat, Arnaud Dupuis, Nadine Marpaux, Christian Naegelen, Guillaume Mourey, Herve Isola, Michel Laforêt, Pascal Morel, and Christian Gachet
Vox Sanguinis, 2018
[2]
T. Sinegre, C. Duron, T. Lecompte, B. Pereira, S. Massoulier, G. Lamblin, A. Abergel, and A. Lebreton
Journal of Thrombosis and Haemostasis, 2018
[3]
Xia Liu, Xiaogang Chen, Jiezuan Yang, and Renyong Guo
Oncology Letters, 2017, Volume 14, Number 3, Page 3787
[4]
Jan F. Vojacek
Clinical and Applied Thrombosis/Hemostasis, 2016, Page 107602961667373

Comments (0)

Please log in or register to comment.
Log in