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Clinical Chemistry and Laboratory Medicine (CCLM)

Published in Association with the European Federation of Clinical Chemistry and Laboratory Medicine (EFLM)

Editor-in-Chief: Plebani, Mario

Ed. by Gillery, Philippe / Greaves, Ronda / Lackner, Karl J. / Lippi, Giuseppe / Melichar, Bohuslav / Payne, Deborah A. / Schlattmann, Peter


IMPACT FACTOR 2018: 3.638

CiteScore 2018: 2.44

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1437-4331
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Volume 54, Issue 3

Issues

Fetal exposure to ethanol: relationship between ethyl glucuronide in maternal hair during pregnancy and ethyl glucuronide in neonatal meconium

Xavier Joya
  • Unitat de Recerca Infància i Entorn (URIE), Institut Hospital del Mar d’Investigacions Mèdiques (IMIM), Barcelona, Spain
  • Red de Salud Materno-Infantil y del Desarrollo (SAMID), Programa RETICS, Instituto Carlos III, Madrid, Spain
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/ Emilia Marchei
  • Department of Therapeutic Research and Medicines Evaluation, Istituto Superiore di Sanitá, Rome, Italy
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/ Judith Salat-Batlle
  • Unitat de Recerca Infància i Entorn (URIE), Institut Hospital del Mar d’Investigacions Mèdiques (IMIM), Barcelona, Spain
  • Red de Salud Materno-Infantil y del Desarrollo (SAMID), Programa RETICS, Instituto Carlos III, Madrid, Spain
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Oscar García-Algar
  • Unitat de Recerca Infància i Entorn (URIE), Institut Hospital del Mar d’Investigacions Mèdiques (IMIM), Barcelona, Spain
  • Red de Salud Materno-Infantil y del Desarrollo (SAMID), Programa RETICS, Instituto Carlos III, Madrid, Spain
  • Departament de Pediatria, Obstetrícia i Ginecologia i Medicina Preventiva, Universitat Autònoma de Barcelona (UAB), Bellaterra, Barcelona, Spain
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/ Valeria Calvaresi
  • Department of Therapeutic Research and Medicines Evaluation, Istituto Superiore di Sanitá, Rome, Italy
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/ Roberta Pacifici
  • Department of Therapeutic Research and Medicines Evaluation, Istituto Superiore di Sanitá, Rome, Italy
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/ Simona Pichini
  • Corresponding author
  • Department of Therapeutic Research and Medicines Evaluation, Istituto Superiore di Sanitá, Rome, Italy
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  • De Gruyter OnlineGoogle Scholar
Published Online: 2015-08-13 | DOI: https://doi.org/10.1515/cclm-2015-0516

Abstract

Background: In recent years, fatty acid ethyl esters (FAEEs) and ethyl glucuronide (EtG) in meconium emerged as reliable, direct biological markers for establishing gestational ethanol exposure. We investigated whether EtG in maternal hair measured during the three trimesters of pregnancy correlated with EtG and FAEEs in neonatal meconium.

Methods: In a prospective sample of 80 mother-infant dyads from Barcelona (Spain), we measured EtG and FAEE in maternal hair segments and meconium samples using a validated UHPLC-MS/MS method.

Results: Fifty-eight (72.5%) women had EtG concentrations in the hair shafts >7 pg/mg in one or more pregnancy trimesters, and EtG and FAEEs in meconium samples were documented in 50 and 24 of their neonates, respectively. The best significant correlations (p<0.0001) were found between EtG concentration in the proximal 0–3 and 3–6 hair shaft segments corresponding to the last two pregnancy trimesters and EtG in neonatal meconium (ρ=0.609 and ρ=0.577, respectively). Using the combination of EtG in meconium ≥30 ng/g and a median of EtG >11 pg/mg in maternal hair during the second and third trimesters of pregnancy, prenatal ethanol exposure could be predicted with a sensitivity of 85.7% and specificity of 73.7%.

Conclusions: This study provides evidence of proven fetal exposure to ethanol during the second and third trimesters of pregnancy by linking detection of ethanol biomarkers (EtG) in maternal hair segments and EtG in neonatal meconium.

Keywords: alcohol biomarkers; fetal alcohol syndrome; hair testing; meconium; pregnancy

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About the article

Corresponding author: Simona Pichini, PhD, Department of Therapeutic Research and Medicines Evaluation, Istituto Superiore di Sanitá, Viale Regina Elena 299, 00161 Rome, Italy, Phone: +39 064 9906545, Fax: +39 064 9902016, E-mail:


Received: 2015-06-04

Accepted: 2015-07-18

Published Online: 2015-08-13

Published in Print: 2016-03-01


Citation Information: Clinical Chemistry and Laboratory Medicine (CCLM), Volume 54, Issue 3, Pages 427–435, ISSN (Online) 1437-4331, ISSN (Print) 1434-6621, DOI: https://doi.org/10.1515/cclm-2015-0516.

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