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Clinical Chemistry and Laboratory Medicine (CCLM)

Published in Association with the European Federation of Clinical Chemistry and Laboratory Medicine (EFLM)

Editor-in-Chief: Plebani, Mario

Ed. by Gillery, Philippe / Lackner, Karl J. / Lippi, Giuseppe / Melichar, Bohuslav / Payne, Deborah A. / Schlattmann, Peter

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Volume 54, Issue 6


Measurement of free light chains with assays based on monoclonal antibodies

Henk te Velthuis
  • Sanquin Reagents and Sanquin Research, Sanquin Blood Supply Foundation and Landsteiner Laboratory, Academic Medical Center, Amsterdam, The Netherlands
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Mark Drayson / John P. Campbell
Published Online: 2016-03-24 | DOI: https://doi.org/10.1515/cclm-2015-0963


Recently, serum free light chain (FLC) assays incorporating anti-kappa (κ) and anti-lambda (λ) FLC monoclonal antibodies have become available: N Latex FLC assay (Siemens) and Seralite® (Abingdon Health). The purpose of this review is to provide an overview of these two new monoclonal antibody-based methods. In doing so, the review will outline the performance characteristics of each method, including a summary of: assay principles, antibody specificity, analytical performance and assay performance in disease. Additionally, the review will describe the potential user benefits of adopting these new generation FLC assays, which are designed to overcome the established limitations of existing polyclonal antibody based FLC assays.

Keywords: free light chains; myeloma; point of care; turbidimetry


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About the article

Corresponding author: John P. Campbell, Institute of Immunology and Immunotherapy, University of Birmingham, Birmingham B15 2TT, UK

Received: 2015-10-02

Accepted: 2016-02-18

Published Online: 2016-03-24

Published in Print: 2016-06-01

Citation Information: Clinical Chemistry and Laboratory Medicine (CCLM), Volume 54, Issue 6, Pages 1005–1014, ISSN (Online) 1437-4331, ISSN (Print) 1434-6621, DOI: https://doi.org/10.1515/cclm-2015-0963.

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