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Clinical Chemistry and Laboratory Medicine (CCLM)

Published in Association with the European Federation of Clinical Chemistry and Laboratory Medicine (EFLM)

Editor-in-Chief: Plebani, Mario

Ed. by Gillery, Philippe / Lackner, Karl J. / Lippi, Giuseppe / Melichar, Bohuslav / Payne, Deborah A. / Schlattmann, Peter / Tate, Jillian R.

12 Issues per year


IMPACT FACTOR 2017: 3.556

CiteScore 2017: 2.34

SCImago Journal Rank (SJR) 2017: 1.114
Source Normalized Impact per Paper (SNIP) 2017: 1.188

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1437-4331
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Volume 56, Issue 1

Issues

Determination of serum calcium levels by 42Ca isotope dilution inductively coupled plasma mass spectrometry

Bingqing Han
  • National Center for Clinical Laboratories, Beijing Hospital, National Center of Gerontology, Beijing Engineering Research Center of Laboratory Medicine, No. 1 Dahua Road, Dongcheng District, Beijing 100730, P.R. China
  • Chinese Academy of Medical Sciences and Peking Union Medical College, No. 9 Dongdan Santiao Road, Beijing 100005, P.R. China
  • Other articles by this author:
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/ Menglei Ge
  • National Center for Clinical Laboratories, Beijing Hospital, National Center of Gerontology, Beijing Engineering Research Center of Laboratory Medicine, No. 1 Dahua Road, Dongcheng District, Beijing 100730, P.R. China
  • Department of Clinical Laboratory, Children’s Hospital, Capital Institute of Pediatrics, No.2 Yabao Road, Chaoyang District, Beijing 10020, P.R. China
  • Other articles by this author:
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/ Haijian Zhao
  • National Center for Clinical Laboratories, Beijing Hospital, National Center of Gerontology, Beijing Engineering Research Center of Laboratory Medicine, No. 1 Dahua Road, Dongcheng District, Beijing 100730, P.R. China
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/ Ying Yan
  • National Center for Clinical Laboratories, Beijing Hospital, National Center of Gerontology, Beijing Engineering Research Center of Laboratory Medicine, No. 1 Dahua Road, Dongcheng District, Beijing 100730, P.R. China
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Jie Zeng
  • National Center for Clinical Laboratories, Beijing Hospital, National Center of Gerontology, Beijing Engineering Research Center of Laboratory Medicine, No. 1 Dahua Road, Dongcheng District, Beijing 100730, P.R. China
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Tianjiao Zhang
  • National Center for Clinical Laboratories, Beijing Hospital, National Center of Gerontology, Beijing Engineering Research Center of Laboratory Medicine, No. 1 Dahua Road, Dongcheng District, Beijing 100730, P.R. China
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/ Weiyan Zhou
  • National Center for Clinical Laboratories, Beijing Hospital, National Center of Gerontology, Beijing Engineering Research Center of Laboratory Medicine, No. 1 Dahua Road, Dongcheng District, Beijing 100730, P.R. China
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  • De Gruyter OnlineGoogle Scholar
/ Jiangtao Zhang
  • National Center for Clinical Laboratories, Beijing Hospital, National Center of Gerontology, Beijing Engineering Research Center of Laboratory Medicine, No. 1 Dahua Road, Dongcheng District, Beijing 100730, P.R. China
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/ Jing Wang
  • National Center for Clinical Laboratories, Beijing Hospital, National Center of Gerontology, Beijing Engineering Research Center of Laboratory Medicine, No. 1 Dahua Road, Dongcheng District, Beijing 100730, P.R. China
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/ Chuanbao Zhang
  • Corresponding author
  • National Center for Clinical Laboratories, Beijing Hospital, National Center of Gerontology, Beijing Engineering Research Center of Laboratory Medicine, 1# Dahua Road, Dongcheng District, Beijing, P.R. China
  • Chinese Academy of Medical Sciences and Peking Union Medical College, No. 9 Dongdan Santiao Road, Beijing 100005, P.R. China, Phone: +8601058115059
  • Email
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Published Online: 2017-07-12 | DOI: https://doi.org/10.1515/cclm-2017-0175

Abstract

Background: Serum calcium level is an important clinical index that reflects pathophysiological states. However, detection accuracy in laboratory tests is not ideal; as such, a high accuracy method is needed.

Methods:

We developed a reference method for measuring serum calcium levels by isotope dilution inductively coupled plasma mass spectrometry (ID ICP-MS), using 42Ca as the enriched isotope. Serum was digested with 69% ultrapure nitric acid and diluted to a suitable concentration. The 44Ca/42Ca ratio was detected in H2 mode; spike concentration was calibrated by reverse IDMS using standard reference material (SRM) 3109a, and sample concentration was measured by a bracketing procedure. We compared the performance of ID ICP-MS with those of three other reference methods in China using the same serum and aqueous samples.

Results:

The relative expanded uncertainty of the sample concentration was 0.414% (k=2). The range of repeatability (within-run imprecision), intermediate imprecision (between-run imprecision), and intra-laboratory imprecision were 0.12%–0.19%, 0.07%–0.09%, and 0.16%–0.17%, respectively, for two of the serum samples. SRM909bI, SRM909bII, SRM909c, and GBW09152 were found to be within the certified value interval, with mean relative bias values of 0.29%, −0.02%, 0.10%, and −0.19%, respectively. The range of recovery was 99.87%–100.37%. Results obtained by ID ICP-MS showed a better accuracy than and were highly correlated with those of other reference methods.

Conclusions:

ID ICP-MS is a simple and accurate candidate reference method for serum calcium measurement and can be used to establish and improve serum calcium reference system in China.

Keywords: high accuracy; isotope dilution inductively coupled plasma mass spectrometry (ID ICP-MS); method comparison; serum calcium

Correction note

Correction added after online publication August 23, 2017: Mistakenly this article was previously published online ahead of print containing a wrong name for 1 author: Yin Yan. The correct name is: Ying Yan

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About the article

Bingqing Han and Menglei Ge are co-first authors.


Received: 2017-02-28

Accepted: 2017-05-24

Published Online: 2017-07-12

Published in Print: 2017-11-27


Author contributions: All the authors have accepted responsibility for the entire content of this submitted manuscript and approved submission.

Research funding: National High Technology Research and Development Program of China: 2011AA01A102, 2011AA02A116.

Employment or leadership: None declared.

Honorarium: None declared.

Competing interests: The funding organization(s) played no role in the study design; in the collection, analysis, and interpretation of data; in the writing of the report; or in the decision to submit the report for publication.


Citation Information: Clinical Chemistry and Laboratory Medicine (CCLM), Volume 56, Issue 1, Pages 51–58, ISSN (Online) 1437-4331, ISSN (Print) 1434-6621, DOI: https://doi.org/10.1515/cclm-2017-0175.

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