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Clinical Chemistry and Laboratory Medicine (CCLM)

Published in Association with the European Federation of Clinical Chemistry and Laboratory Medicine (EFLM)

Editor-in-Chief: Plebani, Mario

Ed. by Gillery, Philippe / Greaves, Ronda / Lackner, Karl J. / Lippi, Giuseppe / Melichar, Bohuslav / Payne, Deborah A. / Schlattmann, Peter


IMPACT FACTOR 2018: 3.638

CiteScore 2018: 2.44

SCImago Journal Rank (SJR) 2018: 1.191
Source Normalized Impact per Paper (SNIP) 2018: 1.205

Online
ISSN
1437-4331
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Volume 56, Issue 6

Issues

Is the DNA of placental origin packaged in exosomes isolated from plasma and serum of pregnant women?

Gabriela Repiská
  • Corresponding author
  • Institute of Physiology, Faculty of Medicine, Comenius University in Bratislava, Sasinkova 2, 813 72 Bratislava, Slovakia
  • Email
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Barbora Konečná
  • Institute of Molecular Biomedicine, Faculty of Medicine, Comenius University in Bratislava, Bratislava, Slovakia
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Ganesh V. Shelke / Cecilia Lässer / Barbora Izrael Vlková
  • Institute of Molecular Biomedicine, Faculty of Medicine, Comenius University in Bratislava, Bratislava, Slovakia
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Gabriel Minárik
  • Department of Molecular Biology, Faculty of Natural Sciences, Comenius University in Bratislava, Bratislava, Slovakia
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
Published Online: 2018-01-08 | DOI: https://doi.org/10.1515/cclm-2017-0560

References

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    Lobb RJ, Becker M, Wen SW, Wong CS, Wiegmans AP, Leimgruber A, et al. Optimized exosome isolation protocol for cell culture supernatant and human plasma. J Extracell Vesicles 2015;4:27031.Web of SciencePubMedCrossrefGoogle Scholar

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    Repiska G, Sedlackova T, Szemes T, Celec P, Minarik G. Selection of the optimal manual method of cell free fetal DNA isolation from maternal plasma. Clin Chem Lab Med 2013;51:1185–9.PubMedWeb of ScienceGoogle Scholar

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    Wong FC, Sun K, Jiang P, Cheng YK, Chan KC, Leung TY, et al. Cell-free DNA in maternal plasma and serum: a comparison of quantity, quality and tissue origin using genomic and epigenomic approaches. Clin Biochem 2016;49:1379–86.CrossrefPubMedWeb of ScienceGoogle Scholar

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    Lui YY, Chik KW, Chiu RW, Ho CY, Lam CW, Lo YM. Predominant hematopoietic origin of cell-free DNA in plasma and serum after sex-mismatched bone marrow transplantation. Clin Chem 2002;48:421–7.PubMedGoogle Scholar

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    Manokhina I, Singh TK, Penaherrera MS, Robinson WP. Quantification of cell-free DNA in normal and complicated pregnancies: overcoming biological and technical issues. PLoS One 2014;9:e101500.CrossrefPubMedWeb of ScienceGoogle Scholar

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    Shelke GV, Jang SC, Yin Y, Lasser C, Lotvall J. Human mast cells release extracellular vesicle-associated DNA. Matters 2016;2:e201602000034.Google Scholar

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    Lazaro-Ibanez E, Sanz-Garcia A, Visakorpi T, Escobedo-Lucea C, Siljander P, Ayuso-Sacido A, et al. Different gDNA content in the subpopulations of prostate cancer extracellular vesicles: apoptotic bodies, microvesicles, and exosomes. Prostate 2014;74:1379–90.CrossrefPubMedWeb of ScienceGoogle Scholar

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    Mitchell MD, Peiris HN, Kobayashi M, Koh YQ, Duncombe G, Illanes SE, et al. Placental exosomes in normal and complicated pregnancy. Am J Obstet Gynecol 2015;213:S173–81.CrossrefPubMedWeb of ScienceGoogle Scholar

About the article

Corresponding author: Mgr. Gabriela Repiská, PhD, Institute of Physiology, Faculty of Medicine, Comenius University in Bratislava, Sasinkova 2, 813 72 Bratislava, Slovakia


Received: 2017-06-28

Accepted: 2017-12-06

Published Online: 2018-01-08

Published in Print: 2018-05-24


Authors contributions: All the authors have accepted responsibility for entire content of this submitted manuscript and approved submission.

Research funding: The study was supported by Grant Agency of Ministry of Education, Science, Research and Sport of the Slovak Republic (VEGA-1/0064/17) and by funding from the Comenius University in Bratislava, Slovakia (UK/102/2015).

Employed or leadership: None declared.

Honorarium: None declared.

Competing interest: The funding organization(s) played no role in the study design; in the collection, analysis, and interpretation of data; in the writing of the report; or in the decision to submit the report for publication.


Citation Information: Clinical Chemistry and Laboratory Medicine (CCLM), Volume 56, Issue 6, Pages e150–e153, ISSN (Online) 1437-4331, ISSN (Print) 1434-6621, DOI: https://doi.org/10.1515/cclm-2017-0560.

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