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Clinical Chemistry and Laboratory Medicine (CCLM)

Published in Association with the European Federation of Clinical Chemistry and Laboratory Medicine (EFLM)

Editor-in-Chief: Plebani, Mario

Ed. by Gillery, Philippe / Greaves, Ronda / Lackner, Karl J. / Lippi, Giuseppe / Melichar, Bohuslav / Payne, Deborah A. / Schlattmann, Peter


IMPACT FACTOR 2018: 3.638

CiteScore 2018: 2.44

SCImago Journal Rank (SJR) 2018: 1.191
Source Normalized Impact per Paper (SNIP) 2018: 1.205

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1437-4331
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Volume 57, Issue 7

Issues

Evaluating the diagnostic and prognostic value of serum long non-coding RNA CTC-497E21.4 in gastric cancer

Wei Zong
  • Department of Laboratory Medicine, Affiliated Hospital of Nantong University, Nantong, P.R. China
  • Other articles by this author:
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/ Wei Feng
  • Department of Laboratory Medicine, Affiliated Hospital of Nantong University, Nantong, P.R. China
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  • De Gruyter OnlineGoogle Scholar
/ Yun Jiang
  • Department of Laboratory Medicine, Affiliated Hospital of Nantong University, Nantong, P.R. China
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/ Shaoqing Ju
  • Department of Laboratory Medicine, Affiliated Hospital of Nantong University, Nantong, P.R. China
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/ Ming Cui
  • Corresponding author
  • Department of Laboratory Medicine, Affiliated Hospital of Nantong University, No 20, Xisi Road, Nantong 226001, P.R. China
  • Email
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/ Rongrong Jing
  • Corresponding author
  • Department of Laboratory Medicine, Affiliated Hospital of Nantong University, No 20, Xisi Road, Nantong 226001, P.R. China
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Published Online: 2019-01-07 | DOI: https://doi.org/10.1515/cclm-2018-0929

Abstract

Background

Long non-coding RNAs (lncRNAs) have been reported to play a key role in gastric cancer (GC) tumorigenesis. However, the clinical application value of serum lncRNAs in GC has remained largely unknown. We investigated the role of a novel lncRNA named CTC-497E21.4 in the diagnosis and the prognosis of GC.

Methods

We focused on evaluation of lncRNA CTC-497E21.4 by real-time fluorescent quantitative polymerase chain reaction (RTFQ-PCR). The study involved following aspects: (1) confirmation of the higher lncRNA CTC-497E21.4 expression in different types of GC specimens than corresponding controls; (2) evaluation of monitoring tumor dynamics by the serum lncRNA CTC-497E21.4 assay; (3) evaluation of the prognostic value of lncRNA CTC-497E21.4 assay in GC.

Results

(1) The method of RTFQ-PCR detection of lncRNA CTC-497E21.4 was evaluated to have high sensitivity and specificity. (2) The expression levels of lncRNA CTC-497E21.4 were higher in GC patients compared with corresponding controls (p<0.001), and the combination of serum lncRNA CTC-497E21.4, CEA and CA19-9 could improve diagnostic sensitivity (96.36%). (3) The serum lncRNA CTC-497E21.4 expression levels were lower in postoperative samples than preoperative samples (p=0.0021) and survival curves downloaded from TCGA showed high lncRNA CTC-497E21.4 levels were associated with poor OS of GC (p=0.0351).

Conclusions

lncRNA CTC-497E21.4 may be a potential biomarker for the diagnosis and the prognosis of GC.

Keywords: biomarker; gastric cancer; lncRNA CTC-497E21.4; long non-coding RNA; serum

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About the article

Received: 2018-08-27

Accepted: 2018-11-26

Published Online: 2019-01-07

Published in Print: 2019-06-26


Author contributions: All the authors have accepted responsibility for the entire content of this submitted manuscript and approved submission.

Research funding: This study was supported by the National Nature Science Foundation (Grant numbers 81201349, 81000775); Young medical key talents in Jiangsu province (Grant numbers QNRC 2016686, 2016687); Frontier and key technical innovation projects of Nantong (Grant number MS22015049); Nantong Science and Technology Plan Project (MS12017008-3).

Employment or leadership: None declared.

Honorarium: None declared.

Competing interests: The funding organization(s) played no role in the study design; in the collection, analysis, and interpretation of data; in the writing of the report; or in the decision to submit the report for publication.


Citation Information: Clinical Chemistry and Laboratory Medicine (CCLM), Volume 57, Issue 7, Pages 1063–1072, ISSN (Online) 1437-4331, ISSN (Print) 1434-6621, DOI: https://doi.org/10.1515/cclm-2018-0929.

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