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Clinical Chemistry and Laboratory Medicine (CCLM)

Published in Association with the European Federation of Clinical Chemistry and Laboratory Medicine (EFLM)

Editor-in-Chief: Plebani, Mario

Ed. by Gillery, Philippe / Greaves, Ronda / Lackner, Karl J. / Lippi, Giuseppe / Melichar, Bohuslav / Payne, Deborah A. / Schlattmann, Peter

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Volume 57, Issue 7


Increased prevalence of anti-DFS70 antibodies in young females: experience from a large international multi-center study on blood donors

Roger Albesa / Ulrich Sachs
  • Institute for Clinical Immunology and Transfusion Medicine, Justus Liebig University, Giessen, Germany
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/ Maria Infantino
  • Immunology and Allergology Laboratory Unit, S. Giovanni di Dio Hospital, Laboratory Medicine, Florence, Italy
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/ Mariangela Manfredi
  • Immunology and Allergology Laboratory Unit, S. Giovanni di Dio Hospital, Laboratory Medicine, Florence, Italy
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/ Maurizio Benucci / Yvonne Baus / Silke Lutterbeck / Luis AndradeORCID iD: https://orcid.org/0000-0001-8742-9931 / Kieran Morris / Alice Friedenberg / Silvia Casas / Xavier Bossuyt
  • KU Leuven Department of Microbiology and Immunology, Leuven, Belgium
  • University Hospitals Leuven, Department of Laboratory Medicine, Leuven, Belgium
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/ Michael Mahler
  • Corresponding author
  • Inova Diagnostics, INC, 9900 Old Grove Road, San Diego, CA 32131-1638, USA, Phone/Fax: +858 586 9900/858 586 9911
  • Email
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Published Online: 2019-02-11 | DOI: https://doi.org/10.1515/cclm-2018-1233



Isolated antibodies to DFS70 have been described in healthy individuals and are rarely found in patients with antinuclear antibody-associated autoimmune rheumatic diseases (AARD). However, no data is available on geographic differences in the prevalence of anti-DFS70 antibodies. We aimed to study the prevalence of anti-DFS70 antibodies in blood donor samples from several countries representing various ethnical backgrounds and geographic regions in the world.


Sera from apparently healthy blood donors (n≥300 per site) were collected in seven countries (USA, Italy, Spain, Germany, UK, Belgium and Brazil). All samples (n=2628) were tested for anti-DFS70 antibodies by QUANTA Flash DFS70 (Inova Diagnostics, Inc., San Diego, CA, USA).


The prevalence of anti-DFS70 antibodies varied from 4/321 (1.2%, Italy) to 42/497 (8.5%, USA). Consequently, the prevalence of the antibodies was significantly higher in USA compared to all other countries (p<0.05). In addition, the prevalence in the combined cohort (all sites) was higher in young blood donors (<35 years; 5.0% vs. 2.7%; p=0.0017) and among females (4.5% vs. 3.0%; p=0.0446). However, when cohorts from different countries were corrected for age and gender, no significant difference between the countries were found.


This is the first study to analyze the prevalence of anti-DFS70 antibodies in different geographic areas using a standardized assay. Our findings show that the antibodies are most prevalent in young females.

Keywords: antinuclear antibodies; autoantibodies; autoimmune disease; DFS; LEDGF


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About the article

Received: 2018-11-08

Accepted: 2018-12-27

Published Online: 2019-02-11

Published in Print: 2019-06-26

Author contributions: All the authors have accepted responsibility for the entire content of this submitted manuscript and approved submission.

Research funding: X. Bossuyt received consultant fees from Inova Diagnostics.

Employment or leadership: R. Albesa, M. Mahler, Y. Baus, S. Lutterbeck and S. Casas are employed at Inova Diagnostics selling autoantibody assays.

Honorarium: None declared.

Competing interests: The funding organization(s) played no role in the study design; in the collection, analysis, and interpretation of data; in the writing of the report; or in the decision to submit the report for publication.

Citation Information: Clinical Chemistry and Laboratory Medicine (CCLM), Volume 57, Issue 7, Pages 999–1005, ISSN (Online) 1437-4331, ISSN (Print) 1434-6621, DOI: https://doi.org/10.1515/cclm-2018-1233.

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