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Clinical Chemistry and Laboratory Medicine (CCLM)

Published in Association with the European Federation of Clinical Chemistry and Laboratory Medicine (EFLM)

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Volume 53, Issue 7

Issues

Reference interval for immature platelet fraction on Sysmex XN hematology analyzer: a comparison study with Sysmex XE-2100

Young Jin Ko
  • Department of Laboratory Medicine, Asan Medical Center, Ulsan University College of Medicine, Seoul, Republic of Korea
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/ Mina Hur
  • Corresponding author
  • Department of Laboratory Medicine, Konkuk University School of Medicine, Seoul, Republic of Korea
  • Email
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/ Hanah Kim
  • Department of Laboratory Medicine, Konkuk University School of Medicine, Seoul, Republic of Korea
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/ Sang Gyeu Choi
  • Department of Laboratory Medicine, Konkuk University School of Medicine, Seoul, Republic of Korea
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/ Hee-Won Moon
  • Department of Laboratory Medicine, Konkuk University School of Medicine, Seoul, Republic of Korea
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/ Yeo-Min Yun
  • Department of Laboratory Medicine, Konkuk University School of Medicine, Seoul, Republic of Korea
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Published Online: 2014-12-02 | DOI: https://doi.org/10.1515/cclm-2014-0839

Abstract

Background: Recently introduced hematology analyzer, the Sysmex XN modular system (Sysmex, Kobe, Japan), has newly adopted a florescent channel to detect platelets and immature platelet fraction (IPF). This study aimed to establish new reference intervals for %-IPF and absolute number of IPF (A-IPF) on Sysmex XN. Platelet counts, %-IPF, and A-IPF were also compared between Sysmex XN and XE-2100 systems (Sysmex).

Methods: Except outliers, blood samples from 2104 healthy individuals and 140 umbilical cord blood were analyzed using both Sysmex XN and XE-2100. The results of two systems were compared using Bland-Altman plot. The reference intervals for %-IPF and A-IPF were defined using non-parametric percentile methods according to the Clinical and Laboratory Standard Institute guideline (C28-A3).

Results: The platelet counts, %-IPF, and A-IPF showed non-parametric distributions. The mean difference between Sysmex XN and XE-2100 in healthy individuals revealed a positive bias in platelets (+8.0×109/L), %-IPF (+1.2%), and A-IPF (+3.0×109/L). The reference intervals for %-IPF and A-IPF on Sysmex XN were: 1.0%–7.3% and 2.49–15.64×109/L in healthy individuals; and 1.0%–4.4% and 2.94–12.82×109/L in umbilical cord blood.

Conclusions: This large-scale study demonstrates a clear difference of platelet counts and IPF between Sysmex XN and XE-2100. The new reference intervals for IPF on Sysmex XN would provide fundamental data for clinical practice and future research.

Keywords: immature platelet fraction; reference interval; Sysmex XN

References

  • 1.

    Ault KA, Knowles C. In vivo biotinylation demonstrates that reticulated platelets are the youngest platelets in circulation. Exp Hematol 1995;23:996–1001.PubMedGoogle Scholar

  • 2.

    Matic GB, Chapman ES, Zaiss M, Rothe G, Schmitz G. Whole blood analysis of reticulated platelets: improvements of detection and assay stability. Cytometry 1998;34:229–34.CrossrefPubMedGoogle Scholar

  • 3.

    Robinson MS, Mackie IJ, Khair K, Liesner R, Goodall AH, Savidge GF, et al. Flow cytometric analysis of reticulated platelets: evidence for a large proportion of non-specific labelling of dense granules by fluorescent dyes. Br J Haematol 1998;100:351–7.Google Scholar

  • 4.

    Pons I, Monteagudo M, Lucchetti G, Muñoz L, Perea G, Colomina I, et al. Correlation between immature platelet fraction and reticulated platelets. Usefulness in the etiology diagnosis of thrombocytopenia. Eur J Haematol 2010;85:158–63.PubMedWeb of ScienceGoogle Scholar

  • 5.

    Michur H, Maślanka K, Szczepiński A, Mariańska B. Reticulated platelets as a marker of platelet recovery after allogeneic stem cell transplantation. Int J Lab Hematol 2008;30:519–25.Web of SciencePubMedGoogle Scholar

  • 6.

    Abe Y, Wada H, Tomatsu H, Sakaguchi A, Nishioka J, Yabu Y, et al. A simple technique to determine thrombopoiesis level using immature platelet fraction (IPF). Thromb Res 2006;118:463–9.PubMedCrossrefGoogle Scholar

  • 7.

    Briggs C, Harrison P, Machin SJ. Continuing developments with the automated platelet count. Int J Lab Hematol 2007;29: 77–91.PubMedWeb of ScienceCrossrefGoogle Scholar

  • 8.

    Briggs C, Kunka S, Hart D, Oguni S, Machin SJ. Assessment of an immature platelet fraction (IPF) in peripheral thrombocytopenia. Br J Haematol 2004;126:93–9.Google Scholar

  • 9.

    Hoffmann JJ, van den Broek NM, Curvers J. Reference intervals of reticulated platelets and other platelet parameters and their associations. Arch Pathol Lab Med 2013;137:1635–40.Web of ScienceGoogle Scholar

  • 10.

    Jung H, Jeon HK, Kim HJ, Kim SH. Immature platelet fraction: establishment of a reference interval and diagnostic measure for thrombocytopenia. Korean J Lab Med 2010;30:451–9.Web of ScienceCrossrefPubMedGoogle Scholar

  • 11.

    Meintker L, Haimerl M, Ringwald J, Krause SW. Measurement of immature platelets with Abbott CD-Sapphire and Sysmex XE-5000 in haematology and oncology patients. Clin Chem Lab Med 2013;51:2125–31.Web of ScienceGoogle Scholar

  • 12.

    Barsam SJ, Psaila B, Forestier M, Page LK, Sloane PA, Geyer JT, et al. Platelet production and platelet destruction: assessing mechanisms of treatment effect in immune thrombocytopenia. Blood 2011;117:5723–32.PubMedWeb of ScienceCrossrefGoogle Scholar

  • 13.

    Ko YJ, Kim H, Hur M, Choi SG, Moon HW, Yun YM, et al. Establishment of reference interval for immature platelet fraction. Int J Lab Hematol 2013;35:528–33.PubMedCrossrefGoogle Scholar

  • 14.

    Briggs C, Longair I, Kumar P, Singh D, Machin SJ. Performance evaluation of the Sysmex haematology XN modular system. J Clin Pathol 2012;65:1024–30.PubMedWeb of ScienceCrossrefGoogle Scholar

  • 15.

    Seo JY, Lee ST, Kim SH. Performance evaluation of the new hematology analyzer Sysmex XN-series. Int J Lab Hematol [Epub ahead of print 12 May 2014]. doi: 10.1111/ijlh.12254.CrossrefGoogle Scholar

  • 16.

    Kim H, Hur M, Choi SG, Moon HW, Yun YM, Hwang HS, et al. Performance evaluation of Sysmex XN hematology analyzer in umbilical cord blood: a comparison study with Sysmex XE-2100. Clin Chem Lab Med 2014;52:1771–9.Google Scholar

  • 17.

    van der Linden N, Klinkenberg LJ, Meex SJ, Beckers EA, de Wit NC, Prinzen L. Immature platelet fraction measured on the Sysmex XN hemocytometer predicts thrombopoietic recovery after autologous stem cell transplantation. Eur J Haematol 2014;93:150–6.Web of ScienceCrossrefGoogle Scholar

  • 18.

    Reed AH, Henry RJ, Mason WB. Influence of statistical method used on the resulting estimate of normal range. Clin Chem 1971;17:275–84.PubMedGoogle Scholar

  • 19.

    Tukey JW. Exploratory data analysis. Reading, MA: Addison-Wesley, 1977:688.Google Scholar

  • 20.

    Horowitz GL, Altaie S, Boyd JC, Ceriotti F, Garg U, Horn P, et al. Defining, establishing, and verifying reference intervals in the clinical laboratory; approved guideline – 3rd ed. Clinical and Laboratory Standard Institute document C28-A3. CLSI: Wayne, PA, 2008.Google Scholar

  • 21.

    Bat T, Leitman SF, Calvo KR, Chauvet D, Dunbar CE. Measurement of the absolute immature platelet number reflects marrow production and is not impacted by platelet transfusion. Transfusion 2013;53:1201–4.PubMedWeb of ScienceCrossrefGoogle Scholar

  • 22.

    Estcourt LJ, Stanworth SJ, Harrison P, Powter G, McClure M, Murphy MF, et al. Prospective observational cohort study of the association between thromboelastometry, coagulation and platelet parameters and bleeding in patients with haematological malignancies – The ATHENA study. Br J Haematol 2014;166:581–91.Web of ScienceGoogle Scholar

  • 23.

    Greene LA, Chen S, Seery C, Imahiyerobo AM, Bussel JB. Beyond the platelet count: immature platelet fraction and thromboelastometry correlate with bleeding in patients with immune thrombocytopenia. Br J Haematol 2014;166: 592–600.Google Scholar

  • 24.

    Siest G, Henny J, Gräsbeck R, Wilding P, Petitclerc C, Queraltó JM, et al. The theory of reference values: an unfinished symphony. Clin Chem Lab Med 2013;51:47–64.PubMedWeb of ScienceGoogle Scholar

  • 25.

    Sola-Visner M, Saxonhouse MA, Brown RE. Neonatal thrombocytopenia: what we do and don’t know. Early Hum Dev 2008;84:499–506.CrossrefWeb of ScienceGoogle Scholar

  • 26.

    Saxonhouse MA, Rimsza LM, Stevens G, Jouei N, Christensen RD, Sola MC. Effects of hypoxia on megakaryocyte progenitors obtained from the umbilical cord blood of term and preterm neonates. Biol Neonate 2006;89:104–8.CrossrefPubMedGoogle Scholar

  • 27.

    Yuko S, Takeda T, Hirota A, Hisaeda Y, Amakata S, Nakao A, et al. Examination of the percentage of immature platelet fraction in term and preterm infants at birth. J Clin Neonatol 2013;2:173–8.PubMedCrossrefGoogle Scholar

  • 28.

    Seo A, Yuan D, Daniels S, Yuan S, Gallagher M, Wong EC. Reference intervals for immature platelet fraction and immature platelet count. Int J Lab Hematol [Epub ahead of print 2014 Apr 17]. doi: 10.1111/ijlh.12237.CrossrefGoogle Scholar

  • 29.

    Schoorl M, Schoorl M, Oomes J, van Pelt J. New fluorescent method (PLT-F) on Sysmex XN2000 hematology analyzer achieved higher accuracy in low platelet counting. Am J Clin Pathol 2013;140:495–9.CrossrefWeb of ScienceGoogle Scholar

  • 30.

    Tanaka Y, Tanaka Y, Gondo K, Maruki Y, Kondo T, Asai S, et al. Performance evaluation of platelet counting by novel fluorescent dye staining in the XN-Series automated hematology analyzers. J Clin Lab Anal 2014;28:341–8.Web of ScienceCrossrefPubMedGoogle Scholar

About the article

Corresponding author: Mina Hur, Department of Laboratory Medicine, Konkuk University School of Medicine, Konkuk University Hospital, 120-1, Neungdong-ro, Hwayang-dong, Gwangjin-gu, Seoul 143-729, Republic of Korea, Phone: +82 2 2030 5581, Fax: +82 2 2636 6764, E-mail:


Received: 2014-08-20

Accepted: 2014-10-29

Published Online: 2014-12-02

Published in Print: 2015-06-01


Citation Information: Clinical Chemistry and Laboratory Medicine (CCLM), Volume 53, Issue 7, Pages 1091–1097, ISSN (Online) 1437-4331, ISSN (Print) 1434-6621, DOI: https://doi.org/10.1515/cclm-2014-0839.

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