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Clinical Chemistry and Laboratory Medicine (CCLM)

Published in Association with the European Federation of Clinical Chemistry and Laboratory Medicine (EFLM)

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Ed. by Gillery, Philippe / Greaves, Ronda / Lackner, Karl J. / Lippi, Giuseppe / Melichar, Bohuslav / Payne, Deborah A. / Schlattmann, Peter


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Diagnostic performance of cerebrospinal fluid free light chains in Lyme neuroborreliosis – a pilot study

Ivar Tjernberg
  • Corresponding author
  • Department of Clinical and Experimental Medicine, Linköping University, Linköping, Sweden
  • Department of Clinical Chemistry and Transfusion Medicine, Region Kalmar County, Kalmar, Sweden
  • Email
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Marcus Johansson
  • Department of Clinical and Experimental Medicine, Linköping University, Linköping, Sweden
  • Department of Clinical Microbiology, Region Kalmar County, Kalmar, Sweden
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Anna J. Henningsson
  • Department of Clinical and Experimental Medicine, Linköping University, Linköping, Sweden
  • Clinical Microbiology, Region Jönköping County, Jönköping, Sweden
  • Clinical Microbiology, Region Östergötland, Linköping, Sweden
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
Published Online: 2019-06-13 | DOI: https://doi.org/10.1515/cclm-2019-0315

Abstract

Background

The aim of this study was to evaluate the diagnostic performance of cerebrospinal fluid (CSF) free light chains (FLCs) in the diagnosis of Lyme neuroborreliosis (LNB).

Methods

Serum and CSF levels of κ- and λ-FLC, albumin and total concentration of immunoglobulin M (IgM) were determined together with CSF chemokine CXCL13 in 23 patients with definite LNB, 35 inflammatory neurological disease control (INDC) and 18 non-inflammatory control (NIC) patients. Indices and intrathecal fractions (IFs) of FLC and IgM were calculated.

Results

Significant differences in FLC indices and IFs were found between the LNB group and both control groups, p ≤ 0.007. Sensitivity of intrathecal κ- and λ-FLC synthesis reached 78%–87% in LNB patients with a specificity of 94%–100% in NIC patients, whereas specificity in INDC patients was 69%. The corresponding frequencies of positive results for IF and index of IgM and CSF CXCL13 in these three diagnostic groups were 74%–96% in LNB patients, 0% in NIC patients and 3%–6% in INDC patients at the chosen cut-off levels.

Conclusions

The findings of this study show a moderate to high sensitivity of CSF κ- and λ-FLC in LNB patients with a high specificity in NIC patients. However, overlap in CSF κ- and λ-FLC levels between LNB and INDC patients calls for caution in the interpretation and limits the diagnostic usefulness in the LNB diagnosis. CSF CXCL13 appears to be the most valuable additional biomarker of LNB aside from routine parameters such as CSF pleocytosis and anti-Borrelia antibody index.

Keywords: cerebrospinal fluid; diagnosis; free light chain; Lyme neuroborreliosis

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About the article

Corresponding author: Ivar Tjernberg, MD, PhD, Department of Clinical and Experimental Medicine, Linköping University, Linköping, Sweden; and Department of Clinical Chemistry and Transfusion Medicine, Region Kalmar County, Kalmar, Sweden, Phone: +46 480 83150


Received: 2019-03-21

Accepted: 2019-05-18

Published Online: 2019-06-13


Author contributions: I. Tjernberg participated in the conception and design of the study, acquisition and statistical analysis of the data and in drafting of the manuscript. M. Johansson participated in acquisition of the data and in reviewing of the manuscript for intellectual content. A.J. Henningsson participated in reviewing of the manuscript for intellectual content. All the authors have accepted responsibility for the entire content of this submitted manuscript and approved submission.

Research funding: This study was conducted with support from the Medical Research Council of Southeast Sweden (FORSS).

Employment or leadership: None declared.

Honorarium: None declared.

Competing interests: The funding organization(s) played no role in the study design; in the collection, analysis, and interpretation of data; in the writing of the report; or in the decision to submit the report for publication.


Citation Information: Clinical Chemistry and Laboratory Medicine (CCLM), 20190315, ISSN (Online) 1437-4331, ISSN (Print) 1434-6621, DOI: https://doi.org/10.1515/cclm-2019-0315.

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