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Current Directions in Biomedical Engineering

Joint Journal of the German Society for Biomedical Engineering in VDE and the Austrian and Swiss Societies for Biomedical Engineering

Editor-in-Chief: Dössel, Olaf

Editorial Board: Augat, Peter / Buzug, Thorsten M. / Haueisen, Jens / Jockenhoevel, Stefan / Knaup-Gregori, Petra / Kraft, Marc / Lenarz, Thomas / Leonhardt, Steffen / Malberg, Hagen / Penzel, Thomas / Plank, Gernot / Radermacher, Klaus M. / Schkommodau, Erik / Stieglitz, Thomas / Urban, Gerald A.


CiteScore 2018: 0.47

Source Normalized Impact per Paper (SNIP) 2018: 0.377

Open Access
Online
ISSN
2364-5504
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Bioprinting of three dimensional tumor models: a preliminary study using a low cost 3D printer

Christian Polley
  • Corresponding author
  • University of Rostock, Fluid Technology and Microfluidics, Justus-von-Liebig Weg 6, 18059 Rostock, Germany
  • Rostock University Medical Center Rostock, Institute for Experimental Surgery, Schillingallee 69a, 18057 Rostock, Germany
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  • De Gruyter OnlineGoogle Scholar
/ Robert Mau / Clemens Lieberwirth / Jan Stenzel
  • Rostock University Medical Center Rostock, Core Facility Multimodal Imaging, Schillingallee 69a, 18057 Rostock, Germany
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/ Brigitte Vollmar
  • Rostock University Medical Center Rostock, Institute for Experimental Surgery, Schillingallee 69a, 18057 Rostock, Germany
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/ Hermann Seitz
Published Online: 2017-09-07 | DOI: https://doi.org/10.1515/cdbme-2017-0028

Abstract

The deep understanding of cancer and tumor genesis, as well as the development of new therapy strategies still remains one of the emerging challenges in modern medicine. To meet these challenges it seems to be absolutely necessary to overcome the drawbacks of the established 2D in vitro models. Especially the missing microenvironment of the tumor, which means the absence of stroma and immune cells, results in a missing cell-cell and cell-stroma interaction as well as disrupted functional communication pathways. Modern 3D culture systems and 3D printing or rather bioprinting technologies attempt to solve this issue and aim to closely mimic natural tumor microenvironment. In this preliminary work we are going to present the first steps of establishing an artificial 3D tumor model utilising a low cost 3D printer. Therefore the printer had been modified with an open-source syringe pump to become a functional bioprinter using viscosity modulated alginate hydrogel. In the first attempts L929 mouse fibroblasts, which are an integral component of natural stroma, had been incorporated into the hydrogel matrix and printed into scaffolds. Subsequent to the printing process the scaffolds got ionically crosslinked with a 5% w/v aqueous solution of CaCl2 to become mechanically stable. After three days of cultivation viability testing had been performed by utilising FDG staining and PET CT to obtain a volumetric viability measurement. The viability imaging showed vital cells homogeneously distributed in the scaffold and therefore stands as an evidence for a working low cost bioprinting process and a successful first step for the development of an artificial 3D tumor model.

Keywords: 3D printing; bioprinting; hydrogel; tumor; cancer

About the article

Published Online: 2017-09-07


Citation Information: Current Directions in Biomedical Engineering, Volume 3, Issue 2, Pages 135–138, ISSN (Online) 2364-5504, DOI: https://doi.org/10.1515/cdbme-2017-0028.

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©2017 Christian Polley et al., published by De Gruyter, Berlin/Boston. This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License. BY-NC-ND 4.0

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