The pressure drop over a pathological vessel section can be used as an important diagnostic indicator. However, it cannot be measured non-invasively. Multiple approaches for pressure reconstruction based on velocity information are available. Regarding in-vivo data introducing uncertainty these approaches may not be robust and therefore validation is required. Within this study, three independent methods to calculate pressure losses from velocity fields were implemented and compared: A three dimensional and a one dimensional method based on the Pressure Poisson Equation (PPE) as well as an approach based on the work-energy equation for incompressible fluids (WERP). In order to evaluate the different approaches, phantoms from pure Computational Fluid Dynamics (CFD) simulations and in-vivo PC-MRI measurements were used. The comparison of all three methods reveals a good agreement with respect to the CFD pressure solutions for simple geometries. However, for more complex geometries all approaches lose accuracy. Hence, this study demonstrates the need for a careful selection of an appropriate pressure reconstruction algorithm.