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Current Directions in Biomedical Engineering

Joint Journal of the German Society for Biomedical Engineering in VDE and the Austrian and Swiss Societies for Biomedical Engineering

Editor-in-Chief: Dössel, Olaf

Editorial Board: Augat, Peter / Buzug, Thorsten M. / Haueisen, Jens / Jockenhoevel, Stefan / Knaup-Gregori, Petra / Kraft, Marc / Lenarz, Thomas / Leonhardt, Steffen / Malberg, Hagen / Penzel, Thomas / Plank, Gernot / Radermacher, Klaus M. / Schkommodau, Erik / Stieglitz, Thomas / Urban, Gerald A.


CiteScore 2018: 0.47

Source Normalized Impact per Paper (SNIP) 2018: 0.377

Open Access
Online
ISSN
2364-5504
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Microfluidic system for enhanced cardiac tissue formation

Mathias Busek / Tomasz Kolanowski
  • TU Dresden, Faculty of Medicine Carl Gustav Carus, Institute of Pharmacology and Toxicology, Fetscherstr. 74, 01307 Dresden, Germany
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Stefan Grünzner
  • Fraunhofer IWS Dresden & TU Dresden Institute of manufacturing technology, George-Bähr-Straße 3c 01069 Dresden, Germany
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Christian Steinfelder / Kaomei Guan
  • TU Dresden, Faculty of Medicine Carl Gustav Carus, Institute of Pharmacology and Toxicology, Fetscherstr. 74, 01307 Dresden, Germany
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Frank Sonntag
Published Online: 2017-09-07 | DOI: https://doi.org/10.1515/cdbme-2017-0076

Abstract

Hereby a microfluidic system for cell cultivation is presented in which human pluripotent stem cell-derived cardiomyocytes were cultivated under perfusion. Besides micro-perfusion this system is also capable to produce well-defined oxygen contents, apply defined forces and has excellent imaging characteristics. Cardiomyocytes attach to the surface, start spontaneous beating and stay functional for up to 14 days under perfusion. The cell motion was subsequently analysed using an adapted video analysis script to calculate beating rate, beating direction and contraction or relaxation speed.

Keywords: cell culture; microfluidic system; induced pluripotent stem cells; cardiomyocytes; video analysis

About the article

1 These authors contribute equally to this work

2 These authors share senior authorship


Published Online: 2017-09-07


Citation Information: Current Directions in Biomedical Engineering, Volume 3, Issue 2, Pages 367–370, ISSN (Online) 2364-5504, DOI: https://doi.org/10.1515/cdbme-2017-0076.

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©2017 Mathias Busek et al., published by De Gruyter. This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License. BY-NC-ND 4.0

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