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Current Directions in Biomedical Engineering

Joint Journal of the German Society for Biomedical Engineering in VDE and the Austrian and Swiss Societies for Biomedical Engineering

Editor-in-Chief: Dössel, Olaf

Editorial Board: Augat, Peter / Buzug, Thorsten M. / Haueisen, Jens / Jockenhoevel, Stefan / Knaup-Gregori, Petra / Kraft, Marc / Lenarz, Thomas / Leonhardt, Steffen / Malberg, Hagen / Penzel, Thomas / Plank, Gernot / Radermacher, Klaus M. / Schkommodau, Erik / Stieglitz, Thomas / Urban, Gerald A.


CiteScore 2018: 0.47

Source Normalized Impact per Paper (SNIP) 2018: 0.377

Open Access
Online
ISSN
2364-5504
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Labbag® - a versatile bag-based cultivation system for expansion, differentiation and cryopreservation of human stem cells

Michael M. Gepp / Rowena Duckstein / Fabian Kayatz
  • Fraunhofer Institute for Process Engineering and Packaging IVV, Branch Lab for Processing Machinery and Packaging Technology, 01189 Dresden, Germany
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/ Norbert Rodler / Zuzana Scheuerer / Julia C. Neubauer / Kristina Lachmann / Cornelia Stramm / Andrea Liebmann
  • Fraunhofer Institute for Process Engineering and Packaging IVV, Branch Lab for Processing Machinery and Packaging Technology, 01189 Dresden, Germany
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/ Michael Thomas / Heiko Zimmermann
  • Corresponding author
  • Fraunhofer Institute for Biomedical Engineering IBMT, Joseph-von-Fraunhofer-Weg 1, 66280 Sulzbach, Germany
  • Molecular and Cellular Biotechnology/Nanotechnology, Saarland University, 66123 Saarbrücken, Germany
  • Facultad de Ciencias del Mar, Universidad Católica del Norte, Coquimbo, Chile
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Published Online: 2017-09-07 | DOI: https://doi.org/10.1515/cdbme-2017-0077

Abstract

Novel approaches in regenerative medicine and tissue engineering are highly promoted by human induced pluripotent stem cells. Typical workflows include the expansion, differentiation and/or cryopreservation of these cells. Often, the differentiation, cytotoxicity tests or disease modelling rely on multicellular aggregates. Here, we present the novel bag-based system “Labbag®” to address workflows required for these applications. As a unique feature, the Labbag® provides a chemical spot pattern to allow the formation of several hundreds of hanging droplets within a few seconds with standard laboratory equipment. In this study, we describe the general concept of the Labbag® and show aggregate formation of stem cells as first stem cell workflow successfully transferred.

Keywords: Hanging droplets; aggregates; spheroids; organoids; human pluripotent stem cells; cell based models

About the article

Published Online: 2017-09-07


Citation Information: Current Directions in Biomedical Engineering, Volume 3, Issue 2, Pages 371–374, ISSN (Online) 2364-5504, DOI: https://doi.org/10.1515/cdbme-2017-0077.

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©2017 Michael M. Gepp et al., published by De Gruyter. This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License. BY-NC-ND 4.0

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