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Current Directions in Biomedical Engineering

Joint Journal of the German Society for Biomedical Engineering in VDE and the Austrian and Swiss Societies for Biomedical Engineering

Editor-in-Chief: Dössel, Olaf

Editorial Board: Augat, Peter / Buzug, Thorsten M. / Haueisen, Jens / Jockenhoevel, Stefan / Knaup-Gregori, Petra / Kraft, Marc / Lenarz, Thomas / Leonhardt, Steffen / Malberg, Hagen / Penzel, Thomas / Plank, Gernot / Radermacher, Klaus M. / Schkommodau, Erik / Stieglitz, Thomas / Urban, Gerald A.


CiteScore 2018: 0.47

Source Normalized Impact per Paper (SNIP) 2018: 0.377

Open Access
Online
ISSN
2364-5504
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Experimental and numerical investigations of fluid flow for optimized in vitro stem cell loading in xenografts

Robert Ott
  • Corresponding author
  • Institute for ImplantTechnology and Biomaterials e.V., Friedrich-Barnewitz-Str.4, 18119 Rostock, Germany
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/ Carolin Wüstenhagen
  • Institute for ImplantTechnology and Biomaterials e.V., Friedrich-Barnewitz-Str.4, 18119 Rostock, Germany
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/ Heiner Martin
  • Institute for Biomedical Engineering, Rostock University Medical Center, Friedrich-Barnewitz-Str. 4, 18119 Rostock, Germany
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/ Michael Stiehm
  • Institute for ImplantTechnology and Biomaterials e.V., Friedrich-Barnewitz-Str.4, 18119 Rostock, Germany
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/ Wolfram Schmidt
  • Institute for Biomedical Engineering, Rostock University Medical Center, Friedrich-Barnewitz-Str. 4, 18119 Rostock, Germany
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/ Nadia Einnolf / Bernhard Frerich
  • Department of Oral, Maxillofacial, and Plastic Surgery, Rostock University Medical Center, Schillingallee 35, 18057 Rostock, Germany
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/ Niels Grabow
  • Institute for Biomedical Engineering, Rostock University Medical Center, Friedrich-Barnewitz-Str. 4, 18119 Rostock, Germany
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/ Klaus-Peter Schmitz
  • Institute for ImplantTechnology and Biomaterials e.V., Friedrich-Barnewitz-Str.4, 18119 Rostock, Germany
  • Institute for Biomedical Engineering, Rostock University Medical Center, Friedrich-Barnewitz-Str. 4, 18119 Rostock, Germany
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/ Stefan Siewert
  • Institute for ImplantTechnology and Biomaterials e.V., Friedrich-Barnewitz-Str.4, 18119 Rostock, Germany
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Published Online: 2017-09-07 | DOI: https://doi.org/10.1515/cdbme-2017-0169

Abstract

In dentofacial surgery, augmentation procedures employing xenografts have become a reliable treatment. Recent studies, however, have shown significant enhance-ments of the in vivo bone tissue augmentation using mesenchymal stem cells loaded into bone grafts. We conducted experimental and numerical investigations in flow perfusion systems to determine flow conditions which allow for homogenous stem cell distribution in BioOss Block (Geistlich Pharma AG, Switzerland) xenografts. Pressure gradient-velocity characteristics and flow distributions were investigated experimentally and numerically at steady state flow conditions with Reynolds numbers (Re) ranging from 0.01 ≤ Re ≤ 0.40. Distilled water at 20°C with a dynamic viscosity of 1.002 mPa.s and a density of 998 kg/m3 was used. The geometry utilized in three-dimensional computa-tional fluid dynamics (CFD) simulation was obtained by means of micro-computed tomography (μCT). Results of CFD analysis are in good accordance with experimental data. The comparison of the pressure gradient-velocity characteris-tics for experimental and numerical data yields a relative error of 3.6%. According to Darcy’s law for creeping fluid flow the experimentally determined permeability is 2.55.10-9 m2. Moreover, numerical flow distribution analysis shows an increasingly heterogenic streamline distribution for increasing Reynolds numbers. Experimentally validated CFD simulations introduced in this study provide a tool to assess optimal flow conditions for a homogenous stem cell distribution in perfusion flow systems.

Keywords: bone graft; xenograft; computational fluid dyna-mics; dentofacial surgery; stem cell; perfusion flow system

About the article

Published Online: 2017-09-07


Citation Information: Current Directions in Biomedical Engineering, Volume 3, Issue 2, Pages 799–802, ISSN (Online) 2364-5504, DOI: https://doi.org/10.1515/cdbme-2017-0169.

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©2017 Robert Ott et al., published by De Gruyter. This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License. BY-NC-ND 4.0

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