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Open Chemistry

formerly Central European Journal of Chemistry

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Volume 1, Issue 1


Volume 13 (2015)

13C NMR and mass spectrometry of soil organic matter

Galya Ivanova
  • Institute of Organic Chemistry with Centre of Phytochemistry, Bulgarian Academy of Sciences, 1113, Sofia, Bulgaria
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/ Edward Randall
Published Online: 2003-03-01 | DOI: https://doi.org/10.2478/BF02479254


Liquid state, high resolution 13C NMR spectroscopy and mass spectrometry were used to study the composition and structure of soil organic matter (SOM) using soil extracts from two long-term experiments at the Rothamsted Experimental Station. Both one- and two-dimensional NMR techniques were applied. 13C NMR sub-spectra of the CHn (n=0...3) groups, obtained by the Distortionless Enhancement by Polarisation Transfer (DEPT) technique, were used for the elucidation of the qualitative and quantitative composition of humic and fulvic acids in the soils. The chemical structure of SOM was further analysed at the molecular level through Fast Atom Bombardment Mass Spectrometry (FABMS) and Gas Chromatography-Mass Spectrometry (GC/MS). Humic and fulvic extract results were not only compared to each other, but also to the solid state 13C NMR results for the complete soil sample.

Keywords: soil organic matter; 13C NMR; DEPT; FABMS; GC/MS; Rothamsted soils

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About the article

Published Online: 2003-03-01

Published in Print: 2003-03-01

Citation Information: Open Chemistry, Volume 1, Issue 1, Pages 10–27, ISSN (Online) 2391-5420, DOI: https://doi.org/10.2478/BF02479254.

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