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Open Chemistry

formerly Central European Journal of Chemistry


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Volume 4, Issue 4

Issues

Volume 13 (2015)

Sequencing peptides by electrospray ion-trap mass spectrometry: A useful tool in synthesis of Axinastatin 3

Chenxi Jia
  • Chemical Engineering Research Center, School of Chemical Engineering and Technology, Tianjin University, Tianjin, 300072, People’s Republic of China
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/ Wei Qi
  • Chemical Engineering Research Center, School of Chemical Engineering and Technology, Tianjin University, Tianjin, 300072, People’s Republic of China
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/ Zhimin He
  • Chemical Engineering Research Center, School of Chemical Engineering and Technology, Tianjin University, Tianjin, 300072, People’s Republic of China
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/ Bin Qiao
  • Chemical Engineering Research Center, School of Chemical Engineering and Technology, Tianjin University, Tianjin, 300072, People’s Republic of China
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Published Online: 2006-12-01 | DOI: https://doi.org/10.2478/s11532-006-0028-y

Abstract

Axinastatin 3 as a potential anticancer agent was synthesized by chemical methods. In an electrospray ion-trap mass spectrometer, using one stage of tandem mass spectrometry (MS/MS), the linear peptide intermediate was sequenced via the complementarities of y and b ions. Then, using multistep MS/MS (to MS6), the cyclic peptide was sequenced through sequentially removing one amino acid residue in each stage of MS/MS. The difference of the fragmentation mechanisms and the sequencing approaches between them is discussed.

Keywords: Tandem mass spectrometry; cyclic peptides; fragmentation pathways; collisionally activated decomposition

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About the article

Published Online: 2006-12-01

Published in Print: 2006-12-01


Citation Information: Open Chemistry, Volume 4, Issue 4, Pages 620–631, ISSN (Online) 2391-5420, DOI: https://doi.org/10.2478/s11532-006-0028-y.

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© 2006 Versita Warsaw. This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License. BY-NC-ND 3.0

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