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Open Chemistry

formerly Central European Journal of Chemistry

1 Issue per year


IMPACT FACTOR 2016 (Open Chemistry): 1.027
IMPACT FACTOR 2016 (Central European Journal of Chemistry): 1.460

CiteScore 2016: 0.61

SCImago Journal Rank (SJR) 2016: 0.288
Source Normalized Impact per Paper (SNIP) 2016: 0.735

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ISSN
2391-5420
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Volume 8, Issue 5 (Oct 2010)

Issues

Screen-printed electrodes modified with HRP-zirconium alcoxide film for the development of a biosensor for acetaminophen detection

Veronica Sima
  • Analytical Chemistry Department, Faculty of Pharmacy, “IULIU HAŢIEGANU” University of Medicine and Pharmacy, Cluj-Napoca, 400349, Romania
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/ Cecilia Cristea
  • Analytical Chemistry Department, Faculty of Pharmacy, “IULIU HAŢIEGANU” University of Medicine and Pharmacy, Cluj-Napoca, 400349, Romania
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/ Ede Bodoki
  • Analytical Chemistry Department, Faculty of Pharmacy, “IULIU HAŢIEGANU” University of Medicine and Pharmacy, Cluj-Napoca, 400349, Romania
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/ Gabriela Duţu
  • Analytical Chemistry Department, Faculty of Pharmacy, “IULIU HAŢIEGANU” University of Medicine and Pharmacy, Cluj-Napoca, 400349, Romania
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/ Robert Sāndulescu
  • Analytical Chemistry Department, Faculty of Pharmacy, “IULIU HAŢIEGANU” University of Medicine and Pharmacy, Cluj-Napoca, 400349, Romania
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Published Online: 2010-09-05 | DOI: https://doi.org/10.2478/s11532-010-0070-7

Abstract

The development of a biosensor based on the immobilization of horseradish peroxidase (HRP) within a zirconium alkoxide-polyetilenimine film onto screen-printed electrodes (SPE) for acetaminophen detection and acetaminophen quantification in pharmaceutical products is described. The biosensor operation mode is based on monitoring the amperometric signal produced by the electrochemical reduction of the enzymatically generated electroactive oxidized species of acetaminophen in the presence of hydrogen peroxide. The enzyme immobilization is performed by retention in a polyethylenimine-zirconium alcoxide porous gel film, a technique that offers good entrapping and a protective environment for the biocomponent due to the hydration properties of the immobilization layer. SPEs have the advantage of being easily mass-produced at low costs with superior characteristics in comparison with classical electrode materials. In this configuration, zirconium alkoxide demonstrates its electrocatalytic activity. The biosensor allows the quantification of acetaminophen with a limit of detection of 6.21×10−8 M and a linear range between 4.35×10−7 M and 4.98×10−6 M. Finally, the biosensor is applied to the quantitative analysis of acetaminophen in Perdolan® tablets.

Keywords: Electrochemical biosensor; Horseradish peroxidase; Zirconium alkoxide; SPE; Acetaminophen

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About the article

Published Online: 2010-09-05

Published in Print: 2010-10-01


Citation Information: Open Chemistry, ISSN (Online) 2391-5420, DOI: https://doi.org/10.2478/s11532-010-0070-7.

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© 2010 Versita Warsaw. This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License. BY-NC-ND 3.0

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