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Open Chemistry

formerly Central European Journal of Chemistry


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Volume 8, Issue 6

Issues

Volume 13 (2015)

Determination of galacturonic acid content in pectin from fruit juices by liquid chromatographydiode array detection-electrospray ionization tandem mass spectrometry

Manuela Moreira
  • REQUIMTE — Chemistry and Biochemistry Department, Faculty of Science, University of Porto, 4169-007, Porto, Portugal
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/ Luís Guido
  • REQUIMTE — Chemistry and Biochemistry Department, Faculty of Science, University of Porto, 4169-007, Porto, Portugal
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/ José Cruz / Aquiles Barros
  • REQUIMTE — Chemistry and Biochemistry Department, Faculty of Science, University of Porto, 4169-007, Porto, Portugal
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Published Online: 2010-10-08 | DOI: https://doi.org/10.2478/s11532-010-0101-4

Abstract

A reversed-phase high-performance liquid chromatographic (HPLC) method is applied for the determination of galacturonic acid (GA) of pectins in different commercial fruit juices. The separation was carried out on a C18 column using precolumn derivatization with p-aminobenzoic acid (p-ABA) and UV detection at 304 nm. The identification of GA was confirmed by high-performance liquid chromatography/electrospray ionization tandem mass spectrometry (HPLC-ESI-MS/MS) in positive ion mode. The concentration of GA in the samples analyzed ranged from 12.9 ± 0.5 to 49.4 ± 0.5 mgGA L−1. Amongst the samples analyzed, mango juice was found to be richest in GA content, and therefore a good source of pectins. Detection and quantification limits of the described methodology were 1.2 and 3.9 mg L−1, respectively. Quantitative GA recoveries in the beverages had a range between 90 and 98%. The results showed that the HPLC method proposed was precise and suitable for the identification and quantification of GA in commercial fruit juices.

Keywords: Pectin; Galacturonic acid; p-aminobenzoic acid; Fruit juices; HPLC-ESI-MS/MS

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About the article

Published Online: 2010-10-08

Published in Print: 2010-12-01


Citation Information: Open Chemistry, Volume 8, Issue 6, Pages 1236–1243, ISSN (Online) 2391-5420, DOI: https://doi.org/10.2478/s11532-010-0101-4.

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© 2010 Versita Warsaw. This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License. BY-NC-ND 3.0

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