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Open Chemistry

formerly Central European Journal of Chemistry


IMPACT FACTOR 2018: 1.512
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2391-5420
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Volume 10, Issue 1

Issues

Volume 13 (2015)

Detection of NADH and ethanol at a graphite electrode modified with titania sol-gel/Meldola’s Blue/MWCNT/Nafion nanocomposite film

Jolanta Kochana / Juliusz Adamski
Published Online: 2011-11-24 | DOI: https://doi.org/10.2478/s11532-011-0132-5

Abstract

For electrocatalytic determination of NADH, a graphite electrode modified with titania sol-gel/Meldola’s Blue/MWCNT/Nafion nanocomposite was proposed. The composition of the matrix film was optimised in terms of the content of carbon nanotubes and Nafion. Incorporation of a redox mediator, Meldola’s Blue, into the nanocomposite film enabled electrocatalytic determination of NADH at a low potential, −50 mV. For determination of ethanol, alcohol dehydrogenase (ADH) was immobilized into the matrix layer. Experimental conditions affecting the biosensor response were examined, including enzyme loading, temperature of measurement and pH of background electrolyte. Assessments of the analytical characteristics of the biosensor were performed with respect to sensitivity, limit of detection, operational stability, repeatability and reproducibility. The proposed biosensor showed electrocatalytic activity toward oxidation of ethanol with sensitivity of 2.24 µA L mmol−1, linear range from 0.05 to 1.1 mmol L−1, and limit of detection of 25 µmol L−1. The apparent Michaelis-Menten constant was 1.24 mmol L−1, indicating a high biological affinity of ADH/titania sol-gel/Meldola’s Blue/MWCNT/Nafion electrode for ethanol. The developed biosensor was tested in determinations of ethanol content in alcoholic beverages.

Keywords: MWCNT; Titania sol-gel; Nafion; NADH; ADH

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About the article

Published Online: 2011-11-24

Published in Print: 2012-02-01


Citation Information: Open Chemistry, Volume 10, Issue 1, Pages 224–231, ISSN (Online) 2391-5420, DOI: https://doi.org/10.2478/s11532-011-0132-5.

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© 2011 Versita Warsaw. This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License. BY-NC-ND 3.0

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