Jump to ContentJump to Main Navigation
Show Summary Details
More options …

Open Chemistry

formerly Central European Journal of Chemistry

1 Issue per year

IMPACT FACTOR 2017: 1.425
5-year IMPACT FACTOR: 1.511

CiteScore 2017: 1.45

SCImago Journal Rank (SJR) 2017: 0.349
Source Normalized Impact per Paper (SNIP) 2017: 0.812

Open Access
See all formats and pricing
More options …
Volume 10, Issue 2


Volume 13 (2015)

Optimization study of the catalytic activity of DNAzymes based on telomeric G-quadruplexes

Joanna Kosman / Bernard Juskowiak
Published Online: 2012-01-29 | DOI: https://doi.org/10.2478/s11532-011-0152-1


Optimization studies of the procedure for peroxidase activity measurements with DNAzymes based on telomeric sequences and colorimetric indicator reactions are reported. Effect of metal cation, nature and concentration of surfactant, as well as thermal treatment of G-quadruplex sample are investigated. Nature of metal cation exhibited modest influence on the system performance. Great improvement of enzymatic activity of the telomeric quadruplexes in the presence of Brij 58 surfactant was observed. Further improvement of catalytic activity of the system based on human telomeric sequence was attained by applying a thermal treatment (heating/rapid cooling) procedure to prepare G-quadruplex/hemin complexes.

Keywords: DNAzyme; G-quadruplex; Peroxidase activity; Brij58; Telomerase assay

  • [1] I. Willner, B. Schlyahovsky, M. Zayats, B. Willner, Chem. Soc. Rev. 37, 1153 (2008) http://dx.doi.org/10.1039/b718428jCrossrefGoogle Scholar

  • [2] Y. Xiao, V. Pavlov, T. Niazov, A. Dishon, M. Kotler, I. Wilnner, J. Am. Chem. Soc. 126, 7430 (2004) http://dx.doi.org/10.1021/ja031875rCrossrefGoogle Scholar

  • [3] V. Pavlov, Y. Xiao, R. Gill, A. Dishon, M. Kotler, I. Willner, Anal. Chem. 76, 2152 (2004) http://dx.doi.org/10.1021/ac035219lCrossrefGoogle Scholar

  • [4] K. Dhaene, E.V. Marck, R. Parwaresch, Virchows Arch. 437, 1 (2000) http://dx.doi.org/10.1007/s004280000189CrossrefGoogle Scholar

  • [5] R. Freeman, E. Sharon, C. Teller, A. Henning, Y. Tzfati, I. Willner, ChemBioChem 11, 2362 (2010) http://dx.doi.org/10.1002/cbic.201000512CrossrefGoogle Scholar

  • [6] R. Re, N. Pellegrini, A. Proteggente, A. Pannala, M. Yang, C. Rice-Evans, Free Radic. Biol. Med. 26, 1231 (1999) http://dx.doi.org/10.1016/S0891-5849(98)00315-3CrossrefGoogle Scholar

  • [7] P. Travascio, Y. Liu, D. Sen, Chem. Biol. 5, 505 (1998) http://dx.doi.org/10.1016/S1074-5521(98)90006-0CrossrefGoogle Scholar

  • [8] A. WŁodarczyk, P. Grzybowski, A. Patkowski, A. Dobek, J. Phys. Chem. B 109, 3594 (2005) http://dx.doi.org/10.1021/jp045274dCrossrefGoogle Scholar

  • [9] S. Nagatoishi, Y. Tanaka, K. Tsumoto, Biochem. Biophys. Res. Commun. 352, 812 (2007) http://dx.doi.org/10.1016/j.bbrc.2006.11.088CrossrefGoogle Scholar

  • [10] A.T. Phan, FEBS J. 277, 1107 (2010) http://dx.doi.org/10.1111/j.1742-4658.2009.07464.xCrossrefGoogle Scholar

  • [11] Y. Li, D. Sen, Biochem. 36, 5589 (2001) Google Scholar

  • [12] S. Nakayama, H.O. Sintim, J. Am. Chem Soc. 131, 10320 (2009) http://dx.doi.org/10.1021/ja902951bCrossrefGoogle Scholar

  • [13] X. Cheng, X. Liu, T. Bing, Z. Cao, D. Shangguan, Biochem. 48, 7817 (2009) http://dx.doi.org/10.1021/bi9006786CrossrefGoogle Scholar

  • [14] D. Kong, W. Yang, J. Wu, C. Li, H. Shen, Analyst 135, 321 (2010) http://dx.doi.org/10.1039/b920293eCrossrefGoogle Scholar

About the article

Published Online: 2012-01-29

Published in Print: 2012-04-01

Citation Information: Open Chemistry, Volume 10, Issue 2, Pages 368–372, ISSN (Online) 2391-5420, DOI: https://doi.org/10.2478/s11532-011-0152-1.

Export Citation

© 2012 Versita Warsaw. This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License. BY-NC-ND 3.0

Citing Articles

Here you can find all Crossref-listed publications in which this article is cited. If you would like to receive automatic email messages as soon as this article is cited in other publications, simply activate the “Citation Alert” on the top of this page.

Hidenobu Yaku, Takashi Murashima, Daisuke Miyoshi, and Naoki Sugimoto
Molecules, 2012, Volume 17, Number 9, Page 10586
Rui Rijo Carvalho, Sidharam P. Pujari, Digvijay Gahtory, Elwin X. Vrouwe, Bauke Albada, and Han Zuilhof
Langmuir, 2017
Joanna Kosman, Yu-Tang Wu, Agata Gluszynska, and Bernard Juskowiak
Analytical and Bioanalytical Chemistry, 2014, Volume 406, Number 28, Page 7049
Benjamin T. Roembke, Jingxin Wang, Shizuka Nakayama, Jie Zhou, and Herman O. Sintim
RSC Advances, 2013, Volume 3, Number 18, Page 6305

Comments (0)

Please log in or register to comment.
Log in