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Open Chemistry

formerly Central European Journal of Chemistry

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Volume 11, Issue 10


Volume 13 (2015)

Antioxidant and chemical properties of Inula helenium root extracts

Iuliana Spiridon
  • Department of Natural Polymers, “Petru Poni” Institute of Macromolecular Chemistry, Romanian Academy, Iasi, 700487, Romania
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/ Constantin Nechita / Marius Niculaua / Mihaela Silion
  • Department of Natural Polymers, “Petru Poni” Institute of Macromolecular Chemistry, Romanian Academy, Iasi, 700487, Romania
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/ Alice Armatu / Carmen-Alice Teacă
  • Department of Natural Polymers, “Petru Poni” Institute of Macromolecular Chemistry, Romanian Academy, Iasi, 700487, Romania
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/ Ruxanda Bodîrlău
  • Department of Natural Polymers, “Petru Poni” Institute of Macromolecular Chemistry, Romanian Academy, Iasi, 700487, Romania
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Published Online: 2013-07-19 | DOI: https://doi.org/10.2478/s11532-013-0295-3


The objective of the paper was to investigate the chemical composition of Inula helenium roots extracts and to evaluate the antioxidant potential conferred by the chemical constituents. GC/MS and HPLC/MS techniques were used to characterize two extracts separated from Inula helenium roots by extraction with chloroform and ethyl acetate, respectively. Volatile compounds have been identified by GC from their mass spectra and retention time values, while HPLC identification of phenolic compounds was realized by comparing their retention times, UV and MS spectra with those of standards or literature data. Measurements of antioxidant activity of Inula helenium root extracts showed a variation between them, which can be correlated with the flavonoid and total phenolic contents. Both Inula helenium root extracts contain phenolic acids (caffeic, chlorogenic, dicaffeoyl quinic, hydroxibenzoic), terpenes (alantolactone) and different flavonoids (epicatechin, catechin gallate, ferulic acid-4-O-glucoside, dihydroquercetin pentosyl rutinoside, kaempherol-7-O-dipentoside, quercetin-3-O-β-glucopyranoside). In addition, the study provides preliminary data on the anti-inflammatory activity of Inula helenium root extracts, this being evaluated using the fresh egg albumin as phlogistic agent, and aspirin as reference compound. Root extracts of I. helenium did not exert any significant anti-inflammatory effect on egg albumin-induced rat paw edema.

Keywords: Inula helenium; Phenolics; Flavonoids; Antioxidant activity; Anti-inflammatory activity

  • [1] A. Stojakowska, J. Malarz, S. Zubek, K. Turnau, W. Kisiel, Biochem. System. Ecol. 38, 232 (2010) http://dx.doi.org/10.1016/j.bse.2009.12.011CrossrefGoogle Scholar

  • [2] J. Zhao, Y. Li, Q. Liu, K. Gao, Food Chem. 120, 512 (2010) http://dx.doi.org/10.1016/j.foodchem.2009.10.045CrossrefGoogle Scholar

  • [3] T. Konishi, Y. Shimada, T. Nagao, H. Okabe, T. Konoshima, Biol. Pharm. Bull. 25, 1370 (2002) http://dx.doi.org/10.1248/bpb.25.1370CrossrefGoogle Scholar

  • [4] Z. Stojanović-Radić, Lj. Čomić, N. Radulović, P. Blagojević, M. Denić, A. Miltojević, J. Rajković, T. Mihajilov-Krstev, Eur. J. Clin. Microbiol. Infect. Dis. 31, 015 (2012) Google Scholar

  • [5] N. J. Lawrence, A.T. McGown, J. Nduka, J.A. Hadfield, R.G. Pritchard, Bioorg. Med. Chem. Lett. 11, 429 (2001) http://dx.doi.org/10.1016/S0960-894X(00)00686-7CrossrefGoogle Scholar

  • [6] C.-N. Chen, H.-H. Huang, C.-L. Wu, P. C. Coney Lin, J.T.A. Hsu, H.-P. Hsieh, S.- E. Chuang, G.-M. Lai, Cancer Lett. 246, 237 (2007) http://dx.doi.org/10.1016/j.canlet.2006.03.004CrossrefGoogle Scholar

  • [7] B. Domon, C. Costello, Glycoconjugate J. 5, 397 (2005) http://dx.doi.org/10.1007/BF01049915CrossrefGoogle Scholar

  • [8] Y. F. Li, H.B. Qu, Y.Y. Cheng, J.Pharm. Biomed. Anal. 47, 407 (2008) http://dx.doi.org/10.1016/j.jpba.2007.12.037CrossrefGoogle Scholar

  • [9] Z. Y. Zhu, H. Zhang, L. Zhao, X. Dong, X. Li, Y.F. Chai, G.Q. Zhang, Rapid Commun. Mass Spectrom. 21, 1855 (2007) http://dx.doi.org/10.1002/rcm.3023CrossrefGoogle Scholar

  • [10] M. Kivilompolo, V. Obùrka, T. Hyötyläinen, Anal. Bioanal. Chem. 388, 881 (2007) http://dx.doi.org/10.1007/s00216-007-1298-8CrossrefGoogle Scholar

  • [11] Y. Zhang, G. Xu, R. Jin, L. Xu, Y. Li, J. China Pharm. Univ. 24, 248 (1993) Google Scholar

  • [12] J. Xia, S. Ji, China Pharm. J. 40, 1895 (2005) Google Scholar

  • [13] Y. Huo, H. Shi, W. Li, M. Wang, X. Li, J. Pharm. Biomed. Anal. 51, 942 (2010) http://dx.doi.org/10.1016/j.jpba.2009.09.032CrossrefGoogle Scholar

  • [14] F. Bohlmann, P.K. Mahanta, J. Jakupovic, R.C. Rastogi, A.A. Natu, Phytochemistry 17, 1165 (1978) http://dx.doi.org/10.1016/S0031-9422(00)94308-5CrossrefGoogle Scholar

  • [15] A. Stojakowska, K. Michalska, J. Malarz, Phytochem. Anal. 17, 157 (2006) http://dx.doi.org/10.1002/pca.900CrossrefGoogle Scholar

  • [16] Y. M. Zhao, M.L. Zhang, Q.W. Shi, H. Kiyota, Chem. Biodivers. 3, 371 (2006) http://dx.doi.org/10.1002/cbdv.200690041CrossrefGoogle Scholar

  • [17] W. Blaschek, R. Hänsel, K. Keller, J. Reichling, H. Rimpler, G. Schneider, Hagers Handbuch der Pharmazeutischen Praxis, Vol. 2: Drogen A-K (Springer, Heidelberg, 1998) http://dx.doi.org/10.1007/978-3-642-58928-7CrossrefGoogle Scholar

  • [18] I. Spiridon, R. Bodîrlău, C.A. Teacă, Cent. Eur. J. Biol. 6(3), 388 (2011) http://dx.doi.org/10.2478/s11535-011-0028-6CrossrefGoogle Scholar

  • [19] R. J. Robbins, J. Agric. Food Chem. 51, 2866 (2003) http://dx.doi.org/10.1021/jf026182tCrossrefGoogle Scholar

  • [20] T. Katsube, H. Tabata, Y. Ohta, Y. Yamasaki, E. Anuurad, K. Shiwaku, Y. Yamane, J. Agric. Food Chem. 52, 2391 (2004) http://dx.doi.org/10.1021/jf035372gCrossrefGoogle Scholar

  • [21] W. Li, Y. Gao, J. Zhao, Q. Wang, J. Agric. Food Chem. 55, 8478 (2007) http://dx.doi.org/10.1021/jf071696jCrossrefGoogle Scholar

  • [22] K. Mruthunjaya, V. I. Hukkeri, Pharmacognosy Magazine 4(23), 42 (2008) Google Scholar

  • [23] R. Govindarajan, S. Rastogi, M. Vijayakumar, A. Shirwaikar, A.K.S. Rawat, S. Mehrotra, P. Pushpangadan, Biol. Pharm. Bull. 26, 1424 (2003) http://dx.doi.org/10.1248/bpb.26.1424CrossrefGoogle Scholar

  • [24] K. N. Muko, F.C. Ohiri, Fitoterapia 71, 65 (2000) http://dx.doi.org/10.1016/S0367-326X(99)00123-9CrossrefGoogle Scholar

  • [25] M. Pinelo, M. Rubilar, M. Jerez, J. Sineiro, M.J. Núñez, J. Agric. Food Chem. 53, 2111 (2005) http://dx.doi.org/10.1021/jf0488110CrossrefGoogle Scholar

  • [26] I. Spiridon, S. Colceru, N. Anghel, C.A. Teacă, R. Bodîrlău, A. Armatu, Nat. Prod. Res. 25(27), 1657 (2011) http://dx.doi.org/10.1080/14786419.2010.521502CrossrefGoogle Scholar

  • [27] R. M. Alonso-Salces, K. Ndjoko, E.F. Queiroz, J.R. Ioset, K. Hostettmann, L.A. Berrueta, B. Gallo, F. Vicente, J. Chromatogr. A 1046, 89 (2004) Google Scholar

  • [28] H. S. Lee, B.W. Widmer, In: L.M.L. Nollet (Ed.), Handbook of Food Analysis (Marcel Dekker, New York, 1996) 821 Google Scholar

  • [29] D. Huang, B. Ou, R.L. Prior, J. Agric. Food Chem. 53, 1841 (2005) http://dx.doi.org/10.1021/jf030723cCrossrefGoogle Scholar

  • [30] R. L. Prior, X. Wu, K. Schaich, J. Agric. Food Chem. 53, 4290 (2005) http://dx.doi.org/10.1021/jf0502698CrossrefGoogle Scholar

  • [31] A. Wojdyło, J. Oszmianski, R. Czemerys, Food Chem. 105, 940 (2007) http://dx.doi.org/10.1016/j.foodchem.2007.04.038CrossrefGoogle Scholar

  • [32] A. Stojakowska, B. Kedzia, W. Kisiel, Fitoterapia 76, 687 (2005) http://dx.doi.org/10.1016/j.fitote.2005.05.003CrossrefGoogle Scholar

  • [33] R. Jaiswal, J. Kiprotich, N. Kuhnert, Phytochemistry 72, 781 (2011) http://dx.doi.org/10.1016/j.phytochem.2011.02.027CrossrefGoogle Scholar

  • [34] H. J. You, S.B. Seo, C.S. Seo, Antibacterial and anti-inflammatory compositions with Inula helenium L. extract and water soluble chitosan, US patent 6521268 (2003) Google Scholar

  • [35] C. A. Rice-Evans, N.J. Miller, G. Paganga, Free Rad. Biol. Med. 20, 933 (1996) http://dx.doi.org/10.1016/0891-5849(95)02227-9CrossrefGoogle Scholar

  • [36] J. W. Han, B. G. Lee, Y. K. Kim, J.W. Yoon, H. K. Jin, S. Hong, H. Y. Lee, K. R. Lee, H.W. Lee, Br. J. Pharmacol. 133, 503 (2001) http://dx.doi.org/10.1038/sj.bjp.0704099CrossrefGoogle Scholar

  • [37] E. Middleton Jr., C. Kandaswami, T.C. Theoharides, Pharmacol. Rev. 52, 673 (2000) Google Scholar

  • [38] P. G. Pietta, J. Nat. Prod. 63, 1035 (2000) http://dx.doi.org/10.1021/np9904509CrossrefGoogle Scholar

  • [39] M. Kassim, M. Achoui, M. Mansor, K.M. Yusoff, Fitoterapia 81, 1196 (2010) http://dx.doi.org/10.1016/j.fitote.2010.07.024CrossrefGoogle Scholar

  • [40] C. Rice-Evans, Curr. Med. Chem. 8(7), 797 (2001) http://dx.doi.org/10.2174/0929867013373011CrossrefGoogle Scholar

  • [41] J. S. Flora, Oxid. Med. Cell. Longev. 2(4), 191 (2009) http://dx.doi.org/10.4161/oxim.2.4.9112CrossrefGoogle Scholar

  • [42] M. Di Rosa, D.A. Willoughby, J. Pharm. Pharmacol. 23(4), 297 (1971) http://dx.doi.org/10.1111/j.2042-7158.1971.tb08661.xCrossrefGoogle Scholar

About the article

Published Online: 2013-07-19

Published in Print: 2013-10-01

Citation Information: Open Chemistry, Volume 11, Issue 10, Pages 1699–1709, ISSN (Online) 2391-5420, DOI: https://doi.org/10.2478/s11532-013-0295-3.

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