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Open Chemistry

formerly Central European Journal of Chemistry

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Volume 13, Issue 1


Volume 13 (2015)

Application of QuEChERS - High Performance Liquid Chromatography with Postcolumn Fluorescence Derivatization (HPLC-FLD) method to analyze Eprinomectin B1a residues from a pour-on conditioning in bovine edible tissues

Ana Csuma / Romeo T. Cristina
  • Banat’s University of Agriculture and Veterinary Medicine “King Michael I of Romania” from Timisoara (BUAMVT), 119, Calea Aradului, 300645, Timisoara, Romania. Department of Pharmacology and Pharmacy
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Eugenia Dumitrescu
  • Banat’s University of Agriculture and Veterinary Medicine “King Michael I of Romania” from Timisoara (BUAMVT), 119, Calea Aradului, 300645, Timisoara, Romania. Department of Pharmacology and Pharmacy
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Florin Muselin / Ersilia C. Alexa / Monica Butnariu / Iosif Gergen
Published Online: 2015-02-12 | DOI: https://doi.org/10.1515/chem-2015-0097


A QuEChERS in house method for determining the marker residue of eprinomectin (eprinomectin B1a) by HPLC-FLD in bovine tissues and milk provided from treated animals was developed and applied. Briefly: all samples were extracted with acetonitrile using a dispersive SPE purification stage. The ascertained detection limits were 1 μg kg-1 and the quantification limits 2 μg kg-1. Recoveries on tissue samples fortified in the range of 10 μg kg-1 to 200 μg kg-1 were from 80.0% to 87.2%, with variation coefficients between 2.7% to 10.6%. The confirmation of residues in the purified extracts was made by LC-MS/MS after separation on an XTerra MS C18 (10 cm × 2.1 mm, 3.5 μm) column with a mobile phase of acetonitrile / formic acid 0.1% (97:3, v/v) at a flow rate of 0.2 mL min-1 and MRM monitoring of three characteristic ions (m/z 896.1, m/z 467.9 and m/z 329.9), resulting from the fragmentation of molecular ions [M-H]+ (m/z 914.6) of eprinomectin and the comparison of the abundance ratio of fragmented ions was obtained in the booth, sample and standard at comparative concentrations. In conclusion, this method has proven its advantage and versatility as a routine drug residues analysis method.

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This article offers supplementary material which is provided at the end of the article.

Keywords : Eprinomectin B1a; QuEChERS HPLC-FLD; Bovine edible tissues; Residues


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About the article

Received: 2014-10-29

Accepted: 2015-01-16

Published Online: 2015-02-12

Citation Information: Open Chemistry, Volume 13, Issue 1, ISSN (Online) 2391-5420, DOI: https://doi.org/10.1515/chem-2015-0097.

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© 2015 Ana Csuma et al.. This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License. BY-NC-ND 3.0

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