Jump to ContentJump to Main Navigation
Show Summary Details
More options …

Open Chemistry

formerly Central European Journal of Chemistry

1 Issue per year


IMPACT FACTOR 2016 (Open Chemistry): 1.027
IMPACT FACTOR 2016 (Central European Journal of Chemistry): 1.460

CiteScore 2016: 0.61

SCImago Journal Rank (SJR) 2016: 0.288
Source Normalized Impact per Paper (SNIP) 2016: 0.735

Open Access
Online
ISSN
2391-5420
See all formats and pricing
More options …
Volume 13, Issue 1 (Feb 2015)

Issues

Application of QuEChERS - High Performance Liquid Chromatography with Postcolumn Fluorescence Derivatization (HPLC-FLD) method to analyze Eprinomectin B1a residues from a pour-on conditioning in bovine edible tissues

Ana Csuma / Romeo T. Cristina
  • Banat’s University of Agriculture and Veterinary Medicine “King Michael I of Romania” from Timisoara (BUAMVT), 119, Calea Aradului, 300645, Timisoara, Romania. Department of Pharmacology and Pharmacy
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Eugenia Dumitrescu
  • Banat’s University of Agriculture and Veterinary Medicine “King Michael I of Romania” from Timisoara (BUAMVT), 119, Calea Aradului, 300645, Timisoara, Romania. Department of Pharmacology and Pharmacy
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Florin Muselin / Ersilia C. Alexa / Monica Butnariu / Iosif Gergen
Published Online: 2015-02-12 | DOI: https://doi.org/10.1515/chem-2015-0097

Abstract

A QuEChERS in house method for determining the marker residue of eprinomectin (eprinomectin B1a) by HPLC-FLD in bovine tissues and milk provided from treated animals was developed and applied. Briefly: all samples were extracted with acetonitrile using a dispersive SPE purification stage. The ascertained detection limits were 1 μg kg-1 and the quantification limits 2 μg kg-1. Recoveries on tissue samples fortified in the range of 10 μg kg-1 to 200 μg kg-1 were from 80.0% to 87.2%, with variation coefficients between 2.7% to 10.6%. The confirmation of residues in the purified extracts was made by LC-MS/MS after separation on an XTerra MS C18 (10 cm × 2.1 mm, 3.5 μm) column with a mobile phase of acetonitrile / formic acid 0.1% (97:3, v/v) at a flow rate of 0.2 mL min-1 and MRM monitoring of three characteristic ions (m/z 896.1, m/z 467.9 and m/z 329.9), resulting from the fragmentation of molecular ions [M-H]+ (m/z 914.6) of eprinomectin and the comparison of the abundance ratio of fragmented ions was obtained in the booth, sample and standard at comparative concentrations. In conclusion, this method has proven its advantage and versatility as a routine drug residues analysis method.

Graphical Abstract

This article offers supplementary material which is provided at the end of the article.

Keywords : Eprinomectin B1a; QuEChERS HPLC-FLD; Bovine edible tissues; Residues

References

  • [1] Inchem WHO Food Additives Series 41: eprinomectin., The 50th meeting of the Joint FAO/WHO Expert Committee on Food Additives (JECFA) Geneva, 1998, http://www.inchem.org/ documents/jecfa/jecmono/v041je02.htm Google Scholar

  • [2] Wells R.G., Eprinomectin, In: Residues of Some Veterinary Drugs in Animals and Food: Monographs prepared by the Fiftieth Meeting Joint FAO/WHO Expert Committee on Food Additives (JECFA) Meeting, Rome, 17-26 February 1998, 41, 31, 1999, http://books.google.ro/books?id=jMCHWPfDYf4C&printsec=fr ontcover&hl=ro&source=gbs_ge_summary_r&cad=0#v=onepa ge&q&f=false Google Scholar

  • [3] Macrocyclic Lactones (Veterinary - Systemic), The United States Pharmacopeia Convention Inc., 2006, http://www.windhound. com/eng/macrocyclicLactones.pdf Google Scholar

  • [4] EMEA/MRL/114/96 Final, Eprinomectin – Summary Report, http://www.ema.europa.eu/docs/en_GB/document_library/ Maximum_Residue_Limits_-_Report/2009/11/WC500014177. pdf Google Scholar

  • [5] Commission Regulation (EU) No 37/2010 on pharmacologically active substances and their classification regarding maximum residue limits in foodstuffs of animal origin, http://ec.europa. eu/health/files/mrl/mrl_20101212_consol.pdf Google Scholar

  • [6] Shoop W., Soll M., Ivermectin, abamectin and eprinomectin, In: J. Vercruysse and R.S. Rew, (Eds.), Macrocyclic lactones in antiparasitic therapy, CABI Publishing, Oxon, U.K., 2002. Google Scholar

  • [7] Shoop W.L., DeMontigny P., Fink D.W., Williams J.B., Egerton J.R., Mrozik H., et al., Int. J. Parasitol., 1996, 26, 1227-1235. CrossrefGoogle Scholar

  • [8] Sheridan R., Desjardins L., J. AOAC Int., 2006, 89, 1088-1094. Google Scholar

  • [9] Riviere J.E., Papich M.G., Veterinary Pharmacology and Therapeutics, Ninth Edition, Wiley & Blackwell, Ames, Iowa, USA, 2013. Google Scholar

  • [10] Floate K.D., Can. J. Vet. Res., 2006, 70, 1-10. Google Scholar

  • [11] Merck & Co., Ivomec® Eprinex™ (eprinomectin) Pour-On for Beef and Dairy Cattle, Environmental Assessment, 1996, http://www.fda. gov/downloads/AnimalVeterinary/DevelopmentApprovalProcess/ EnvironmentalAssessments/UCM072375.pdf Google Scholar

  • [12] McKellar Q.A., Gokbulut C., Curr. Pharm. Biotechnol., 2012, 13, 888-991. CrossrefGoogle Scholar

  • [13] Kiki-Mvouaka S., Menez C., Borin C., Lyazrhi F., Foucaud- Vignault M., Dupuy J., Drug. Metab. Dispos., 2010, 38, 573-580. CrossrefGoogle Scholar

  • [14] Alvinerie M., Sutra J.F., Galtier P., Mage C., Res. Vet. Sci., 1999, 67, 229-232. Google Scholar

  • [15] Baoliang P., Yuwan W., Zhende P., Lifschitz A.L., Ming W., Vet. Res. Commun., 2006, 30, 263-270. Google Scholar

  • [16] Bassissi M.F., Alvinerie M., Lespine A., Comp. Biochem. Physiol. C Toxicol. Pharmacol., 2004, 138, 437-444. Google Scholar

  • [17] Bengone-Ndong T., Ba M.A., Kane Y., Sané I., Sutra J.F., Alvinerie M., Parasitol. Res., 2006, 98, 501-506. Google Scholar

  • [18] Wen H., Pan B., Wang Y., Wang F., Yang Z., Wang M., Vet. Parasitol., 2010, 174, 72-76. Google Scholar

  • [19] Sutra J.F., Chartier C., Galtier P., Alvinerie M., Analyst, 1998, 123, 1525-1527. Google Scholar

  • [20] Danaher M., O’Keeffe M., Glennon J., Howells L., Analyst, 2001, 126, 576-580. Google Scholar

  • [21] Ali M.S., Sun T., McLeroy G.E., Phillippo E.T., J AOAC Int., 2000, 83, 31-38. Google Scholar

  • [22] Ali M.S., Sun T., McLeroy G.E., Phillippo E.T., J. AOAC Int., 2000, 83, 39-52. Google Scholar

  • [23] Rudik I., Cummings M.R., Poppenga R.H., J. Vet. Diagn. Invest., 2002, 14, 295-302. CrossrefGoogle Scholar

  • [24] Kinsella B., Lehotay S.J., Mastovska K., Lightfield A.R., Furey A., Danaher M., Chim. Acta, 2009, 637, 196-207. Google Scholar

  • [25] Directive 2004/9/EC of the European parliament and of the Council of 11 February 2004 on the inspection and verification of good laboratory practice (GLP), http://ec.europa.eu/enterprise/ sectors/chemicals/documents/specific-chemicals/laboratorypractice/ index_en.htm Google Scholar

  • [26] Directive 2004/10/EC of the European parliament and of the Council of 11 February 2004 on the harmonisation of laws, regulations and administrative provisions relating to the application of the principles of good laboratory practice and the verification of their applications for tests on chemical substances, http://ec.europa.eu/enterprise/sectors/chemicals/documents/ specific-chemicals/laboratory-practice/index_en.htm Google Scholar

  • [27] Commission Decision 2002/657/EC implementing Council Directive 96/23/EC concerning the performance of analytical methods and the interpretation of results. Google Scholar

  • [28] Jiang H., Ding S., Xu F., Zhao S., He J., Liu J., et al., Chromatographia, 2007, 66, 411-414. Google Scholar

  • [29] Miller J.C., Miller J.N., Statistics for analytical chemistry, Sixth Edition, Gosport, U.K., Ashford Colour Press Ltd., 2010. Google Scholar

  • [30] Durden D.A., J. Chromatogr. B, 2007, 850, 134-146. Google Scholar

  • [31] Ordin nr. 187 din 31 octombrie 2007, pentru aprobarea Normei sanitare veterinare privind Codul produselor medicinale veterinare (In Romanian), 2007, http://www.ansvsa.ro/ documente/Ordin_187_2007_Codul_produselor_medicinale_ veterinare_262ro.pdf Google Scholar

About the article

Received: 2014-10-29

Accepted: 2015-01-16

Published Online: 2015-02-12


Citation Information: Open Chemistry, ISSN (Online) 2391-5420, DOI: https://doi.org/10.1515/chem-2015-0097.

Export Citation

© 2015 Ana Csuma et al.. This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License. BY-NC-ND 3.0

Supplementary Article Materials

Citing Articles

Here you can find all Crossref-listed publications in which this article is cited. If you would like to receive automatic email messages as soon as this article is cited in other publications, simply activate the “Citation Alert” on the top of this page.

[1]
Luisa C. Domingos, Matheus Vilardo L. Moreira, Kelly M. Keller, Fernando Antônio B. Viana, Marília M. Melo, and Benito Soto-Blanco
Journal of Pharmacological and Toxicological Methods, 2017, Volume 83, Page 87

Comments (0)

Please log in or register to comment.
Log in