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Open Chemistry

formerly Central European Journal of Chemistry

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Volume 13, Issue 1


Volume 13 (2015)

Retention, separation selectivity and system efficiency of selected basic psychotropic drugs on different RPLC columns

Anna Petruczynik
  • Corresponding author
  • Department of Inorganic Chemistry Medical University of Lublin,Chodźki 4a, 20-093 Lublin, Poland
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Karol Wróblewski
  • Department of Inorganic Chemistry Medical University of Lublin,Chodźki 4a, 20-093 Lublin, Poland
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Kamil Dzioba
  • Corresponding author
  • Department of Inorganic Chemistry Medical University of Lublin,Chodźki 4a, 20-093 Lublin, Poland
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Monika Waksmundzka-Hajnos
  • Department of Inorganic Chemistry Medical University of Lublin,Chodźki 4a, 20-093 Lublin, Poland
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
Published Online: 2015-06-03 | DOI: https://doi.org/10.1515/chem-2015-0106


Retention parameters of psychotropic drug standards were determined on different columns, i.e., Octadecyl silica, Phenyl, Phenyl-Hexyl, Polar Reverse Phase, Pentafluorophenyl, and Cyanopropyl using aqueous eluent systems containing methanol or acetonitrile as organic modifiers, acetate buffer at pH 3.5 and addition of silanol blocker − diethylamine (DEA).

The retention, separation selectivity, and sequence of elution were different when using eluents containing various organic modifiers. The significant differences were observed in retention parameters with a change of the used stationary phase. The various properties of stationary phases resulted in differences in analyte retention, peaks shape, systems efficiency and separation selectivity. The best shape of peaks were on Cyanopropyl (CN) column and the highest efficiency for most investigated psychotropic drugs were obtained on Phenyl-Hexyl and Polar RP columns.

Graphical Abstract

Keywords : HPLC; psychotropic drugs; silanol blockers; different stationary phases; system efficiency


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About the article

Received: 2014-12-12

Accepted: 2015-04-01

Published Online: 2015-06-03

Citation Information: Open Chemistry, Volume 13, Issue 1, ISSN (Online) 2391-5420, DOI: https://doi.org/10.1515/chem-2015-0106.

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© 2015 Anna Petruczynik et al.. This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License. BY-NC-ND 3.0

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