Jump to ContentJump to Main Navigation
Show Summary Details
More options …

Open Chemistry

formerly Central European Journal of Chemistry

1 Issue per year

IMPACT FACTOR 2016 (Open Chemistry): 1.027
IMPACT FACTOR 2016 (Central European Journal of Chemistry): 1.460

CiteScore 2016: 0.61

SCImago Journal Rank (SJR) 2016: 0.288
Source Normalized Impact per Paper (SNIP) 2016: 0.735

Open Access
Online
ISSN
2391-5420
See all formats and pricing
More options …
Volume 13, Issue 1

Issues

Volume 13 (2015)

Influence of extraction methods on the recovery of astragaloside IV from the roots of Astragalus mongholicus in Soxhlet- and Twisselmann-type apparatus

Wirginia Kukuła-Koch
  • Corresponding author
  • Chair and Department of Pharmacognosy with Medical Plant Unit, Medical University of Lublin, 1, Chodźki Str., 20-093 Lublin, Poland
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Tomasz Baj
  • Chair and Department of Pharmacognosy with Medical Plant Unit, Medical University of Lublin, 1, Chodźki Str., 20-093 Lublin, Poland
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Elwira Sieniawska
  • Chair and Department of Pharmacognosy with Medical Plant Unit, Medical University of Lublin, 1, Chodźki Str., 20-093 Lublin, Poland
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Wojciech Koch
  • Chair and Department of Food and Nutrition, Medical University of Lublin, 4a, Chodźki Str., 20-093 Lublin, Poland
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Jarosław Widelski
  • Chair and Department of Pharmacognosy with Medical Plant Unit, Medical University of Lublin, 1, Chodźki Str., 20-093 Lublin, Poland
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Katarzyna Luchowska
  • Department of Industrial and Medicinal Plants, University of Life Sciences, 15, Akademicka Str., 20-950 Lublin, Poland
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Barbara Kołodziej
  • Department of Industrial and Medicinal Plants, University of Life Sciences, 15, Akademicka Str., 20-950 Lublin, Poland
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Kazimierz Głowniak
  • Chair and Department of Pharmacognosy with Medical Plant Unit, Medical University of Lublin, 1, Chodźki Str., 20-093 Lublin, Poland
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
Published Online: 2015-06-02 | DOI: https://doi.org/10.1515/chem-2015-0108

Abstract

Astragalus mongholicus, commonly used in Traditional Chinese Medicine (TCM), contains significant amounts of triterpene saponins – astragalosides, which possess a wide variety of pharmacological actions, including the treatment of cardiovascular and respiratory diseases. The present study was performed to develop a fast and effective extraction method of saponins from crude plant material using Twisselmann-type apparatus. Moreover, a detailed comparison between Soxhlet and Twisselmann apparatus was performed to explain the advantages and disadvantages of their applications. The content of astragaloside IV – the major saponin of A. mongholicus extracts and chosen as an extraction efficiency marker ‒ was determined by means of RP-HPLCELSD chromatography in an herein optimized method. Twisselmann extraction shortened its duration from 4 to 2 hours in comparison with the Soxhlet method ‒ a generally accepted method preparation for this extract. The effectiveness of both apparatus was alike (3.24 ± 0.44 mg and 3.48 ± 0.26 mg of astragaloside IV/ 4g of powdered root for 2 hours in Twisselmann and 4 hours in Soxhlet apparatus). Compared with a pharmacopoeial method, the application of Twisselmann apparatus enabled a decrease in analysis time of 50%, which is of high significance, especially for large, scaled-up industrial scale.

Graphical Abstract

This article offers supplementary material which is provided at the end of the article.

Keywords : Astragalus mongholicus; Twisselmann extraction; HPLC-ELSD detection; saponins; astragaloside IV

References

  • [1] Anon. Astragalus membranaceus. Altern. Med. Rev., 2007, 8, 72-77. Google Scholar

  • [2] Pharmacopoeia of the People’s Republic of China, Vol. 1, People’s Medical Publishing House, Shenzen, 2001. Google Scholar

  • [3] Yesilada E., Bedir E., Calis I., Takahashi Y., Ohmoto Y., Effects of triterpene saponins from Astragalus species on in vitro cytokine release, J. Ethnopharmacol., 2005, 96, 71-77. CrossrefGoogle Scholar

  • [4] Lin L.Z., He X.G., Lindenmaier M., Nolan G., Yang J., Cleary M., et al., Liquid Chromatography-Electrospray Ionization Mass Spectrometry Study of the Flavonoids of the Roots of Astragalus mongholicus and A. membranaceus, J. Chrom. A, 2000, 876, 1-2, 87-95. Google Scholar

  • [5] Ren S., Zhang H., Mu., Sun M., Liu P., Pharmacological effects of Astragaloside IV: a literature review, J. Tradit. Chin. Med., 2013, 15, 33, 3, 413-416. Google Scholar

  • [6] Hu J.Y., Han J., Chu Z.G., Song H.P., Zhang D.X., Zhang Q., et al., Astragaloside IV attenuates hypoxia-induced cardiomyocyte damage in rats by upregulating superoxide dismutase-1 levels, Clin. Exp. Pharmacol. Physiol., 2009, 36, 4, 351-357. Web of ScienceCrossrefGoogle Scholar

  • [7] Ji K.T., Tang J.F., Chai J.D. Effect of Astragaloside against the oxidative damage on endothelial cells. Zhong Guo Zhong Xi Yi Jie He Za Zhi 2011, 31, 6, 807-810. Google Scholar

  • [8] Li H.B., Gei Y.K., Zhang L., Astragaloside IV improved barrier dysfunction induced by acute high glucose in human umbiIical vein endothehal cells. Life Sci. 2006; 79, 12, 1186-1193. Google Scholar

  • [9] Liu X., Min W., Protective effects of Astragaloside against ultraviolet a-induced photoaging in human fibroblasts, Zhong Xi Yi Jie He Xue Bao, 2011, 9, 3, 328-332. Google Scholar

  • [10] Meng L.Q., Tang J.W., Wang Y., Zhao J.R., Shang M.Y., Zhang M., et al., Astragaloside IV synergizes with ferulic acid to inhibit renal tubulointerstitial fibrosis in rats with obstructive nephropathy, Br. J. Pharmacol., 2011, 162, 8, 1805-1818. Web of ScienceGoogle Scholar

  • [11] Liu H., Wei W., Sun W.Y., Li X., Protective effects of Astragaloside IV on porcine-serum-induced hepatic fibrosis in rats and in vitro effects on hepatic stellate cells, J. Ethnopharmacol., 2009, 122, 3, 502-508. Web of ScienceGoogle Scholar

  • [12] Wang S., Li J., Huang H., Gao W., Zhuang C., Li B., et al., Anti-hepatitis B virus activities of Astragaloside IV isolated from radix Astragali, Biol. Pharm. Bull., 2009, 32, 1, 132-135. CrossrefWeb of ScienceGoogle Scholar

  • [13] European Pharmacopoeia 8.0 edition, 2013. PubMedGoogle Scholar

  • [14] Monschein M., Ardjomand-Woelkart K., Rieder J., Wolf I., Heydel B., Kunert O., et. al., Accelerated sample preparation and formation of astragaloside IV in Astragali radix, Pharm Biol. (In press), DOI: 10.3109/13880209.2013.839712. CrossrefGoogle Scholar

  • [15] Jalsrai A., Grecksch G., Becker A., Evaluation of the effects of Astragalus mongholicus Bunge saponin extract on central nervous system functions, J. Ethnopharmacol., 2010, 131, 3, 544-549. Web of ScienceGoogle Scholar

  • [16] Kloeck G., Noke A., Christiansen T., Soxhlet or Twisselmann, Filtr. Separat., 2013, 33, 32-33. Google Scholar

About the article

Received: 2014-12-03

Accepted: 2015-04-01

Published Online: 2015-06-02


Citation Information: Open Chemistry, Volume 13, Issue 1, ISSN (Online) 2391-5420, DOI: https://doi.org/10.1515/chem-2015-0108.

Export Citation

© 2015 Wirginia Kukuła-Koch et al.. This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License. BY-NC-ND 3.0

Supplementary Article Materials

Comments (0)

Please log in or register to comment.
Log in