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Open Chemistry

formerly Central European Journal of Chemistry

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Volume 13, Issue 1


Volume 13 (2015)

Supercritical carbon dioxide hops extracts with antimicrobial properties

Edward Rój
  • Corresponding author
  • New Chemical Syntheses Institute, Al. Tysiąclecia Państwa Polskiego 13A, 24-110 Puławy, Poland
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Vanja M. Tadić
  • Institute for Medicinal Plant Research “Dr Josif Pančić”, Tadeuša Košćuška 1, 11000 Belgrade, Serbia
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Dušan Mišić
  • University of Belgrade, Faculty of Veterinary Medicine, Bulevar oslobodjenja 18, 11000 Belgrade, Serbia
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Irena Žižović
  • University of Belgrade, Faculty of Technology and Metallurgy, Karnegijeva 4, 11000 Belgrade, Serbia
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Ivana Arsić / Agnieszka Dobrzyńska-Inger
  • New Chemical Syntheses Institute, Al. Tysiąclecia Państwa Polskiego 13A, 24-110 Puławy, Poland
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Dorota Kostrzewa
  • New Chemical Syntheses Institute, Al. Tysiąclecia Państwa Polskiego 13A, 24-110 Puławy, Poland
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
Published Online: 2015-09-11 | DOI: https://doi.org/10.1515/chem-2015-0131


Extracts obtained from hops (Humulus lupulus L., Cannabaceae) by supercritical fluid extraction (SFE), SFE followed by isomerization, as well as by conventional technique, were investigated for their chemical composition and antibacterial activity against selected foodborne pathogens and microorganisms capable to cause the food spoilage. The antibacterial activity of the extracts was compared with the antibacterial activity of xanthohumol, compound known for its broad pharmacological properties, isolated from the raw material remained after the SFE. Xanthohumol (XH, 96%) proved to posses the most prominent activity against all the tested strains, with the MIC values ranged between 2.5 and 20 μg mL-1. Supercritical hops extract and potassium isomerized supercritical hops extract showed strong antibacterial activity against the tested strains as well. Escherichia coli was not affected by the extracts, meaning that their oral admission would not cause the same problem as antibiotic application in intestinal flora. The chemical composition of the investigated hops extracts was analysed by GC-MS. Contents of α-acids, β-acids, iso-α-acids and xanthohumol in the samples were determined by HPLC.

Graphical Abstract

Keywords : supercritical hops extract; isomerized hops extract; supercritical CO2 extraction; xanthohumol; antibacterial activity


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About the article

Received: 2015-02-23

Accepted: 2015-07-27

Published Online: 2015-09-11

Citation Information: Open Chemistry, Volume 13, Issue 1, ISSN (Online) 2391-5420, DOI: https://doi.org/10.1515/chem-2015-0131.

Export Citation

© 2015 Edward Rój et al.. This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License. BY-NC-ND 3.0

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