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Open Chemistry

formerly Central European Journal of Chemistry

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Volume 13, Issue 1


Volume 13 (2015)

Qualitative and quantitative analysis of polyphenolic compounds in Ilex Sp.

Anna Zwyrzykowska
  • Corresponding author
  • Department of Environment Hygiene and Animal Welfare, The Faculty of Biology and Animal Science
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Robert Kupczyński
  • Department of Environment Hygiene and Animal Welfare, The Faculty of Biology and Animal Science
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Bogdan Jarosz / Antoni Szumny / Alicja Z. Kucharska
  • Departament of Fruit, Vegetable and Cereals Technology, The Faculty of Food Science and Technology, Wrocław University of Environmental and Life Sciences
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
Published Online: 2015-11-30 | DOI: https://doi.org/10.1515/chem-2015-0142


Natural compounds are an important source of desired biological activity which help to improve nutritional status, enhance productivity and bring many health benefits. The leaves of the Ilex paraguariensis (Aquifoliaceae) are used for preparing a beverage known as yerba mate and represent a proven source of natural polyphenols which are known to foster biological activity with the emphasis on antioxidant properties. In present work we focused on the polyphenolic content of air-dried leaves of Ilex aquifolium L., Ilex aquifolium ‘Argentea Mariginata’, Ilex meserveae ‘Blue Angel’, and a commercially available mate as the reference product. Liquid chromatography combined with mass spectrometry (HPLC and LC-MS) and thin layer chromatography (TLC), were used to establish polyphenolic substances content in aqueous methanolic extracts obtained from the biological matter. Up to 20 polyphenolic compounds were identified in the extracts, including rutin, quinic acid and its caffeoyl esters, i.e. chlorogenic acid and its isomers as well as dicaffeoyl derivatives. We took chlorogenic acid and rutin as reference compounds to quantify their levels in the extracts. It was determined that in all tested plants, high levels of these antioxidants were present. This led us to the conclusion that their leaves might serve as valuable food additives.

Keywords : Ilex sp.; extraction; polyphenols; HPLC MS


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About the article

Received: 2015-02-15

Accepted: 2015-10-01

Published Online: 2015-11-30

Citation Information: Open Chemistry, Volume 13, Issue 1, ISSN (Online) 2391-5420, DOI: https://doi.org/10.1515/chem-2015-0142.

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© 2015 Anna Zwyrzykowska et al.. This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License. BY-NC-ND 3.0

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