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Open Chemistry

formerly Central European Journal of Chemistry

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Volume 13, Issue 1


Volume 13 (2015)

Production of calcium preparations by technology of saltwater fish by product processing

Fabiola Bubel
  • Corresponding author
  • Department of Environmental Engineering and Industrial Waste Recycling, Poltegor-Institute, 51-616 Wroclaw, Poland
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Zbigniew Dobrzański
  • Department of Environment, Hygiene and Animal Welfare, Wroclaw University of Environmental and Life Science, 51-630 Wroclaw, Poland
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Piotr Jan Bykowski
  • Corresponding author
  • Faculty of Business Administration and Commodity Sciences, Gdynia Maritime University, 81-225 Gdynia, Poland
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Katarzyna Chojnacka
  • Institute of Inorganic Technology and Mineral Fertilizers, Wroclaw University of Technology, 50-372 Wroclaw, Poland
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Sebastian Opaliński
  • Corresponding author
  • Department of Environment, Hygiene and Animal Welfare, Wroclaw University of Environmental and Life Science, 51-630 Wroclaw, Poland
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Tadeusz Trziszka
  • Department of Animal Products Technology and Quality Management, Wroclaw University of Environmental and Life Science, 51-630 Wroclaw, Poland
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
Published Online: 2016-01-18 | DOI: https://doi.org/10.1515/chem-2015-0146


The paper presents the technology of processing Baltic cod (Gadus morhua callarias) and Atlantic salmon (Salmo salar) backbones – by-products from fish processing – for the production of calcium preparations. The raw material for the process consisted of backbones with remaining muscle tissue, devoid of heads and fins (30 kg of each type of backbones). The processing included the following stages: cutting, preliminary processing in an alkaline environment (soaking in 2 M NaOH), processing with 0.1% citric acid, aroma removal and material disinfection (5% H2O2), rinsing with tap water, drying, and grinding the end-product. Calcium preparations from cod (BCP) and salmon (ASP) in the amounts of 1.20 kg and 1.62 kg, respectively, characterized by high calcium content (27.79% in BCP, and 24.92% in ASP) and low protein and fat content (14.20% and 0.25% for BCP, and 10.78% and 0.12% for ASP, respectively) were obtained. The study demonstrated the effectiveness of this technology for production of calcium preparations from fish backbones.

Graphical Abstract

Keywords : Baltic cod; Atlantic salmon; backbones; calcium compounds


  • [1] Hryszko K. (Ed.), Rynek ryb stan i perspektywy, Wyd. IERiGŻ-PIB, Warszawa, 2013, (in Polish). Google Scholar

  • [2] Czapliński P., Funkcjonowanie przemysłu przetwórstwa rybnego w Polsce w okresie kryzysu gospodarczego, Prace Komisji Geografii Przemysłu Polskiego Towarzystwa Geograficznego, 2011, 17, 114-128, (in Polish). Google Scholar

  • [3] Szostak S., Kuzebski E., Budny T., Morska gospodarka rybna w 2005, Wyd. MIR., Gdynia, 2006, (in Polish). Google Scholar

  • [4] Szostak S., Rakowski M., Budny T., Morska gospodarka rybna w 2012, Wyd. MIR., Gdynia, 2013, (in Polish). Google Scholar

  • [5] Ghaly A.E., Ramakrishnan V.V., Brooks M.S., Budge S.M., Dave D., Fish Processing Wastes as a Potential Source of Proteins, Amino Acids and Oils: A Critical Review, J. Microb. Biochem. Technol., 2013, 5(4), 107-129. Google Scholar

  • [6] Liaset B., Julshamn K., Espe M., Chemical composition and nutritional evaluation of the produced fractions from enzymic hydrolysis of salmon frames with ProtamexTM, Process Biochem., 2003, 38(12), 1747-1759. CrossrefGoogle Scholar

  • [7] Rustad T., Storrø I., Slizyte R., Possibilities for the utilisation of marine by-products, Int. J. Food. Sci. Tech., 2011, 46(10), 2001-2014. CrossrefGoogle Scholar

  • [8] Thorkelsson G., Sigurgisladottir S., Geirsdottir M., Jóhannsson R., Guerard F., Chabeaud A., et al., In: In: Borresen T. (Ed.), Improving seafood products for the consumer, Woodhead Publishing Limited, Cambridge, UK, 2008. Google Scholar

  • [9] Bower C. K., Hietala K. A., Acidification methods for stabilization and storage salmon by-products, J. Aquat. Food Prod. Tech., 2008, 17(4), 459-478. CrossrefGoogle Scholar

  • [10] Olsen R.L., Toppe J., Karunasagar I., Challenges and realistic opportunities in the use of by-products from processing of fish and shellfish, Trends Food Sci. Tech., 2014, 36(2), 144-151. CrossrefGoogle Scholar

  • [11] FAO, The State of World Fisheries and Aquaculture 2012, Rome, Italy, 2012. Google Scholar

  • [12] Kim S.K., Mendis E., Bioactive compounds from marine processing byproducts – a review, Food Res. Int., 2006, 39, 383-393. CrossrefGoogle Scholar

  • [13] Kim S.K., Wijesekara I., Development and biological activities of marine-derived bioactive peptides: a review, J. Func. Foods, 2010, 2(1), 1-9. Google Scholar

  • [14] Fiori L., Manfrini M., Castello D., Supercritical CO2 fractionation of omega-3 lipids from fish by-products: Plant and process design, modeling, economic feasibility, Food Bioprod. Process., 2014, 92(2), 120-130. Web of ScienceGoogle Scholar

  • [15] Dolińska B., Zieliński M., Dobrzański Z., Chojnacka K., Opaliński S., Ryszka F., Influence of incubation conditions on hydrolysis efficiency and iodine enrichment in baker’s yeast, Biol. Trace Elem. Res., 2012, 147(1-3), 354-358. Web of ScienceGoogle Scholar

  • [16] Phiraphinyo P., Taepakpurenat S., Lakkanatinaporn P., Suntornsuk W., Suntornsuk L., Physical and chemical properties of fish and chicken bones as calcium source for mineral supplements, Songklanakarin J. Sci. Technol., 2006, 28(2), 327-335. Google Scholar

  • [17] Toppe J., Aksnes A., Hope B., Albrektsen S., Inclusion of fish bone and crab by-products in diets for Atlantic cod, Gadus morhua, Aquaculture, 2006, 253(1-4), 636-645. Google Scholar

  • [18] Tongchan P., Prutipanlai S., Niyomwas S., Thongraung C., Effect of calcium compound obtained from fish byproduct on calcium metabolism in rats, J. Food Ag-Ind., 2009, 2(04), 669-676. Google Scholar

  • [19] Ferraro V., Cruz I.B., Jorge R.F., Malcata F.X., Pintado M.E., Castro P.M.L., Valorisation of natural extracts from marine source focused on marine by-products: a review, Food Res. Int., 2010, 43(9), 2221–2233. Web of ScienceCrossrefGoogle Scholar

  • [20] Boutinguiza M., Pou J., Comesaña R., Lusquiños F., de Carlos A., León B., Biological hydroxyapatite obtained from fish bones, Mat. Sci. Eng. C- Mater., 2012, 32 (3), 478-486. CrossrefGoogle Scholar

  • [21] Gildberg A., Arnsen J.A., Carlehög M., Utilisation of cod backbone by biochemical fractionation, Process Biochem., 2002, 38(4), 475-480. CrossrefGoogle Scholar

  • [22] Barakat N. A. M., Khalil K. A., Sheikh F. A., Omran A. M., Gaihre B., Khil M. S., et al., Physiochemical characterizations of hydroxyapatite extracted from bovine bones by three different methods: extraction of biologically desirable HAp Mat. Sci. Eng. C- Mater., 2008, 28(8), 1381–1387. CrossrefGoogle Scholar

  • [23] Kim S.K., Jung W.K., In: Shahidi F. (Ed.), Maximising the value of marine by-products, Cambridge, Woodhead Publishing Limited, Cambridge, 2006. Google Scholar

  • [24] Jeon Y.J., Byun H.G., Kim S.K., Improvement of functional properties of cod frame protein hydrolysates using ultrafiltration membranes, Process Biochem., 1999, 35(5), 471-478. CrossrefGoogle Scholar

  • [25] Techochatchawal K., Therdthai N., Khotavivattana S., Development of calcium supplement from the bone of Nile Tilapia (Tilapia nilotica), As. J. Food Ag-Ind., 2009, 2(04), 539-546. Google Scholar

  • [26] Jiancong H., Shanggui D., Chao X., Guozhong T., Preparation and biological efficacy of haddock bone calcium tablets, Chin. J. Oceanol. Limnol., 2010, 28(2), 372-378. Web of ScienceGoogle Scholar

  • [27] Malde M.K, Graff I.E., Siljander-Rasi H., Venäläinen E., Julshamn K., Pedersen J.I., et al., Fish bones-a highly available calcium source for growing pigs, J. Anim. Physiol. Anim. Nutr., 2010, 94(5), 66-76. CrossrefWeb of ScienceGoogle Scholar

  • [28] Logesh A.R., Pravinkumar M., Raffi S.M., Kalaiselvam M., Calcium and phosphorus determination in bones of low value fishes, Sardinella longiceps (Valenciennes) and Trichiurus savala (Cuvier), from Parangipettai, Southeast Coast of India, Asian Pac. J. Trop. Dis., 2012, 2(1), 254-256. Google Scholar

  • [29] Skierka E., Sadowska M., Mańkowska J., Lipińska-Beyer A., Enzymatyczna hydroliza białek mięśniowych kręgosłupów dorsza bałtyckiego, Med. Weter., 2008, 64 (5), 677-680, (in Polish). Google Scholar

  • [30] Ramirez A., Salmon by-product proteins, FAO Fisheries Circular No. 1027, FAO, Rome, 2007. Google Scholar

  • [31] Opaliński S., Korczyński M., Kozieł J. A., Bubel F., Dobrzański Z., Fish processing by-products. Olfactometric assessment of chemical deodorization, Przem. Chem., 2013, 92(6), 1159-1162, (in Polish). Google Scholar

  • [32] Skowron K., Bauza-Kaszewska J., Dobrzanski Z., Paluszak Z., Skowron K. J., UV-C radiation as a factor reducing microbiological contamination of fish meal, The Scient. World J., 2014, http://dx.doi.org/10.1155/2014/928094. CrossrefGoogle Scholar

  • [33] Hemung B.O., Properties of Tilapia Bone Powder and Its Calcium Bioavailability Based on Transglutaminase Assay, Int. J. Biosci. Biochem. Bioinforma., 2013, 3(4), 306-309. Google Scholar

  • [34] Huo J.C., Deng S.G., Xie C., Tong G.Z., Preparation of biological efficacy of haddock bone calcium tablets Chin. J. Ocean Limnol., 2010, 28(2), 371-378. Google Scholar

  • [35] Xavier E.L., Pravinkumar M., Logesh A.R., Viswanathan C., Raffi S.M., Calcium and phosphorous levels in bones of three low value fishes Sardinella fimbriata, Sardinella albella and Sardinella gibbosa from Muttom, Southeast Coast of India, Int. J. Cur. Tr. Res., 2014, 3(2), 43-46. Google Scholar

  • [36] Changhu X., Zhaojie L., Cheng S., Zhongliang W., Studies on the preparation of active calcium from pollack frame, J. Ocean Univ. Qingdao, 1995, 25(2), 173-178. Google Scholar

  • [37] Malde M.K, Bügel S., Kristensen M., Malde K., Graff I.E., Pedersen J.I, Calcium from salmon and cod bone is well absorbed in young healthy men: a double-blinded randomised crossover design, Nutr. Metab., 2010, 7:61, http://nutritionandmetabolism.biomedcentral.com/articles/10.1186/1743-7075-7-61. CrossrefGoogle Scholar

  • [38] Commission Regulation (EC) N°1881/2006 of 19 December 2006 setting maximum levels for certain contaminants in foodstuffs with further changes EC no. 629/2008 (Official Journal of the European Union of 20.12.2006, L 364/5 and of 03.07.2008, L 173/6). Google Scholar

  • [39] Regulation of the Minister of Agriculture and Rural Development of 6 February 2012 on the level of undesirable substances in animal feed (Journal of Laws of 2012, item. 203) Google Scholar

  • [40] Dobrzański Z., Bubel F., Chojnacka K., Bykowski P.J., Bolanowski J., Trziszka T., Przem. Chem., Physicochemical properties of solid fish-processing wastes and their uses in nutraceutical and biomedical productions, 2012, 91(5), 733-737, (in Polish). Google Scholar

  • [41] Bubel, F., Bykowski P.J., Chojnacka K., Dobrzański Z., Opaliński S., Trziszka T., 13 marzec 2013, Wapniowy preparat organiczno-mineralny oraz sposób wytwarzania wapniowego preparatu organiczno-mineralnego, Urząd Patentowy RP, zgłoszenie patentowe P.403123, (in Polish). Google Scholar

About the article

Received: 2015-01-30

Accepted: 2015-10-21

Published Online: 2016-01-18

Published in Print: 2015-12-31

Citation Information: Open Chemistry, Volume 13, Issue 1, ISSN (Online) 2391-5420, DOI: https://doi.org/10.1515/chem-2015-0146.

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© 2015 Fabiola Bubel et al.. This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License. BY-NC-ND 3.0

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