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Volume 63, Issue 4


Characterization of recombinant antibodies for detection of TNT and its derivatives

Mladen Simonoviíc / Svetlana Milosević-Zlatanović
  • Faculty of Mathematics and Natural Sciences, University of Kragujevac, Radoja Domanovića 12, 34000, Kragujevac, Serbia
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/ Nenad Milosavić
  • Department of Chemistry, Institute of Chemistry, Technology and Metallurgy, Njegoševa 12, 11000, Belgrade, Serbia
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/ Miroslav Vrvić / Branislav Simonović
Published Online: 2009-05-27 | DOI: https://doi.org/10.2478/s11696-009-0043-5


Diverse recombinant immunoreagents specific for TNT-derivatives were tested in different assay forms in order to analyze their specificity and sensitivity. Performance of immunoassays was based on TNP-protein conjugates immobilization on a solid surface. In this work, the detection limit for TNT-analog TNP-Tris was 250 fmol or 87 pg mL−1 (87 ppt), which represents the most sensitive assay published until now, regarding the detection of recombinant antibodies.

Keywords: TNT; assay; antibody; TNP-Tris; recombinant; detection

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About the article

Published Online: 2009-05-27

Published in Print: 2009-08-01

Citation Information: Chemical Papers, Volume 63, Issue 4, Pages 391–398, ISSN (Online) 1336-9075, DOI: https://doi.org/10.2478/s11696-009-0043-5.

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© 2009 Institute of Chemistry, Slovak Academy of Sciences.

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